Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/36775 |
Resumo: | Nanoparticles (NP) bioactivity is under deep scrutiny. In this work, the antioxidant response to TiO2-NP in wheat (Triticum aestivum) was determined. For that, enzymatic and the non-enzymatic antioxidants were evaluated in plants exposed to the P25 anatase:rutile material composed of TiO2-NP and under environmentally realistic doses (0; 5; 50; 150 mg/L for 20 days). Shoot but not root growth was reduced. In leaves, thiol metabolism and ascorbate accumulation were the preferred route whereas in roots the pre-existing antioxidant capacity was preferentially utilized. Both leaves and roots showed increased glutathione reductase and dehydroascorbate reductase activities and decreased ascorbate peroxidase activity. Roots, nevertheless, presented higher enzymatic basal levels than leaves. On the other hand, when examining non-enzymatic antioxidants, the ratio of reduced-to-oxidized glutathione (GSH/GSSG) increased in leaves and decreased in roots. Exposed leaves also presented higher total ascorbate accumulation compared to roots. TiO2-NP exposure down regulated, with more prominence in roots, antioxidant enzyme genes encoding catalase, ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase. In leaves, superoxide dismutase gene expression was increased. All data pinpoint to TiO2-NP toxicity above 5 mg/L, with aerial parts being more susceptible, which draws concerns on the safety doses for the use of these NPs in agricultural practices. |
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Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependentAntioxidant batteryAntioxidant thiolsCrops-nanoparticle interactionNanoparticles toxicityOxidative stressNanoparticles (NP) bioactivity is under deep scrutiny. In this work, the antioxidant response to TiO2-NP in wheat (Triticum aestivum) was determined. For that, enzymatic and the non-enzymatic antioxidants were evaluated in plants exposed to the P25 anatase:rutile material composed of TiO2-NP and under environmentally realistic doses (0; 5; 50; 150 mg/L for 20 days). Shoot but not root growth was reduced. In leaves, thiol metabolism and ascorbate accumulation were the preferred route whereas in roots the pre-existing antioxidant capacity was preferentially utilized. Both leaves and roots showed increased glutathione reductase and dehydroascorbate reductase activities and decreased ascorbate peroxidase activity. Roots, nevertheless, presented higher enzymatic basal levels than leaves. On the other hand, when examining non-enzymatic antioxidants, the ratio of reduced-to-oxidized glutathione (GSH/GSSG) increased in leaves and decreased in roots. Exposed leaves also presented higher total ascorbate accumulation compared to roots. TiO2-NP exposure down regulated, with more prominence in roots, antioxidant enzyme genes encoding catalase, ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase. In leaves, superoxide dismutase gene expression was increased. All data pinpoint to TiO2-NP toxicity above 5 mg/L, with aerial parts being more susceptible, which draws concerns on the safety doses for the use of these NPs in agricultural practices.Elsevier2023-03-31T14:05:09Z2019-12-15T00:00:00Z2019-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36775eng0304-389410.1016/j.jhazmat.2019.120889Silva, SóniaFerreira de Oliveira, José Miguel P.Dias, Maria CelesteSilva, Artur M. S.Santos, Conceiçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:10:53Zoai:ria.ua.pt:10773/36775Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:28.640531Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
title |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
spellingShingle |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent Silva, Sónia Antioxidant battery Antioxidant thiols Crops-nanoparticle interaction Nanoparticles toxicity Oxidative stress |
title_short |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
title_full |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
title_fullStr |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
title_full_unstemmed |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
title_sort |
Antioxidant mechanisms to counteract TiO2-nanoparticles toxicity in wheat leaves and roots are organ dependent |
author |
Silva, Sónia |
author_facet |
Silva, Sónia Ferreira de Oliveira, José Miguel P. Dias, Maria Celeste Silva, Artur M. S. Santos, Conceição |
author_role |
author |
author2 |
Ferreira de Oliveira, José Miguel P. Dias, Maria Celeste Silva, Artur M. S. Santos, Conceição |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Sónia Ferreira de Oliveira, José Miguel P. Dias, Maria Celeste Silva, Artur M. S. Santos, Conceição |
dc.subject.por.fl_str_mv |
Antioxidant battery Antioxidant thiols Crops-nanoparticle interaction Nanoparticles toxicity Oxidative stress |
topic |
Antioxidant battery Antioxidant thiols Crops-nanoparticle interaction Nanoparticles toxicity Oxidative stress |
description |
Nanoparticles (NP) bioactivity is under deep scrutiny. In this work, the antioxidant response to TiO2-NP in wheat (Triticum aestivum) was determined. For that, enzymatic and the non-enzymatic antioxidants were evaluated in plants exposed to the P25 anatase:rutile material composed of TiO2-NP and under environmentally realistic doses (0; 5; 50; 150 mg/L for 20 days). Shoot but not root growth was reduced. In leaves, thiol metabolism and ascorbate accumulation were the preferred route whereas in roots the pre-existing antioxidant capacity was preferentially utilized. Both leaves and roots showed increased glutathione reductase and dehydroascorbate reductase activities and decreased ascorbate peroxidase activity. Roots, nevertheless, presented higher enzymatic basal levels than leaves. On the other hand, when examining non-enzymatic antioxidants, the ratio of reduced-to-oxidized glutathione (GSH/GSSG) increased in leaves and decreased in roots. Exposed leaves also presented higher total ascorbate accumulation compared to roots. TiO2-NP exposure down regulated, with more prominence in roots, antioxidant enzyme genes encoding catalase, ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase. In leaves, superoxide dismutase gene expression was increased. All data pinpoint to TiO2-NP toxicity above 5 mg/L, with aerial parts being more susceptible, which draws concerns on the safety doses for the use of these NPs in agricultural practices. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-15T00:00:00Z 2019-12-15 2023-03-31T14:05:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/36775 |
url |
http://hdl.handle.net/10773/36775 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0304-3894 10.1016/j.jhazmat.2019.120889 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137729851686912 |