Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73904 |
Resumo: | A novel electrochemical biosensor based on a molecularly imprinted polymer (MIP) was developed for the impedimetric determination of Tau protein, a biomarker of Alzheimers disease (AD). Indeed, a recent correlation between AD symptoms and the presence of Tau proteins in their aggregated form made hyperphosphorylated Tau protein (Tangles) a promising biomarker for Alzheimers diagnosis. The MIP was directly assembled on a screen-printed carbon electrode (C-SPE) and prepared by electropolymerization of 3-aminophenol (AMP) in the presence of the protein template (p-Tau-441) using cyclic voltammetry. The p-Tau-441 protein bound to the polymeric backbone was digested by the action of the proteolytic activity of proteinase K in urea and then washed away to create vacant sites. The performances of the corresponding imprinted and non-imprinted electrodes were evaluated by electrochemical impedance spectroscopy. The detection limit of the MIP-based sensors was 0.02 pM in PBS buffer pH 5.6. Good selectivity and good results in serum samples were obtained with the developed platform. The biosensor described in this work is a potential tool for screening Tau protein on-site and an attractive complement to clinically established methodologies methods as it is easy to fabricate, has a short response time and is inexpensive. |
id |
RCAP_43d80e4f049cce8e488bfadd3330d0e8 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/73904 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detectionElectrochemical biosensorMIPsTau proteinSerumAlzheimer diseaseCiências Naturais::Ciências BiológicasScience & TechnologyA novel electrochemical biosensor based on a molecularly imprinted polymer (MIP) was developed for the impedimetric determination of Tau protein, a biomarker of Alzheimers disease (AD). Indeed, a recent correlation between AD symptoms and the presence of Tau proteins in their aggregated form made hyperphosphorylated Tau protein (Tangles) a promising biomarker for Alzheimers diagnosis. The MIP was directly assembled on a screen-printed carbon electrode (C-SPE) and prepared by electropolymerization of 3-aminophenol (AMP) in the presence of the protein template (p-Tau-441) using cyclic voltammetry. The p-Tau-441 protein bound to the polymeric backbone was digested by the action of the proteolytic activity of proteinase K in urea and then washed away to create vacant sites. The performances of the corresponding imprinted and non-imprinted electrodes were evaluated by electrochemical impedance spectroscopy. The detection limit of the MIP-based sensors was 0.02 pM in PBS buffer pH 5.6. Good selectivity and good results in serum samples were obtained with the developed platform. The biosensor described in this work is a potential tool for screening Tau protein on-site and an attractive complement to clinically established methodologies methods as it is easy to fabricate, has a short response time and is inexpensive.0624_2IQBIONEURO_6_E, 2QBioneuro, Impulso de una red de I + i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas EP-INTERREG V A España Portugal (POCTEP).info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoBen Hassine, AmiraRaouafi, NoureddineMoreira, Felismina2021-08-252021-08-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73904engBen Hassine, Amira; Raouafi, Noureddine; Moreira, Felismina, Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection. Chemosensors, 9(9), 238, 20212227-904010.3390/chemosensors9090238https://www.mdpi.com/2227-9040/9/9/238info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:18Zoai:repositorium.sdum.uminho.pt:1822/73904Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:24.235320Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
title |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
spellingShingle |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection Ben Hassine, Amira Electrochemical biosensor MIPs Tau protein Serum Alzheimer disease Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
title_full |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
title_fullStr |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
title_full_unstemmed |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
title_sort |
Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection |
author |
Ben Hassine, Amira |
author_facet |
Ben Hassine, Amira Raouafi, Noureddine Moreira, Felismina |
author_role |
author |
author2 |
Raouafi, Noureddine Moreira, Felismina |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ben Hassine, Amira Raouafi, Noureddine Moreira, Felismina |
dc.subject.por.fl_str_mv |
Electrochemical biosensor MIPs Tau protein Serum Alzheimer disease Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Electrochemical biosensor MIPs Tau protein Serum Alzheimer disease Ciências Naturais::Ciências Biológicas Science & Technology |
description |
A novel electrochemical biosensor based on a molecularly imprinted polymer (MIP) was developed for the impedimetric determination of Tau protein, a biomarker of Alzheimers disease (AD). Indeed, a recent correlation between AD symptoms and the presence of Tau proteins in their aggregated form made hyperphosphorylated Tau protein (Tangles) a promising biomarker for Alzheimers diagnosis. The MIP was directly assembled on a screen-printed carbon electrode (C-SPE) and prepared by electropolymerization of 3-aminophenol (AMP) in the presence of the protein template (p-Tau-441) using cyclic voltammetry. The p-Tau-441 protein bound to the polymeric backbone was digested by the action of the proteolytic activity of proteinase K in urea and then washed away to create vacant sites. The performances of the corresponding imprinted and non-imprinted electrodes were evaluated by electrochemical impedance spectroscopy. The detection limit of the MIP-based sensors was 0.02 pM in PBS buffer pH 5.6. Good selectivity and good results in serum samples were obtained with the developed platform. The biosensor described in this work is a potential tool for screening Tau protein on-site and an attractive complement to clinically established methodologies methods as it is easy to fabricate, has a short response time and is inexpensive. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-25 2021-08-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73904 |
url |
http://hdl.handle.net/1822/73904 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ben Hassine, Amira; Raouafi, Noureddine; Moreira, Felismina, Novel electrochemical molecularly imprinted polymer-based biosensor for Tau protein detection. Chemosensors, 9(9), 238, 2021 2227-9040 10.3390/chemosensors9090238 https://www.mdpi.com/2227-9040/9/9/238 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132312923799552 |