Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75827 |
Resumo: | Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host. |
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Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the hostBiofilmsVirulenceIronSiderophoresMutagenesisStaphylococcus epidermidisScience & TechnologyIron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host.This study was supported by the Portuguese Foundation for Science and Technology (FCT) through the funded project PTDC/BIAMOL/29553/2017, under the scope of COMPETE2020 (POCI-01-0145-FEDER-029553), and the strategic funding of UID/BIO/04469/2019 unit. This study was also supported through funds from the German Research Council (DFG) and the Damp Foundation. FO was supported by an individual Ph.D. scholarship (SFRH/BD/101399/2014).The authors acknowledge the support of the i3S Scientific Platform HEMS, member of the PPBI (PPBIPOCI-01-0145-FEDER-022122).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoOliveira, Fernando Eduardo FreitasLima, TâniaCorreia, AlexandraSilva, Ana MargaridaSoares, CristinaMorais, SimoneWeißelberg, SamiraVilanova, ManuelRohde, HolgerCerca, Nuno2021-12-242021-12-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75827engOliveira, Fernando E.; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, Samira; Vilanova, Manuel; Rohde, Holger; Cerca, Nuno, Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host. Frontiers in Medicine, 8(799227), 20212296-858X10.3389/fmed.2021.799227https://www.frontiersin.org/articles/10.3389/fmed.2021.799227/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:58Zoai:repositorium.sdum.uminho.pt:1822/75827Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:39.931319Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
title |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
spellingShingle |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host Oliveira, Fernando Eduardo Freitas Biofilms Virulence Iron Siderophores Mutagenesis Staphylococcus epidermidis Science & Technology |
title_short |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
title_full |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
title_fullStr |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
title_full_unstemmed |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
title_sort |
Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host |
author |
Oliveira, Fernando Eduardo Freitas |
author_facet |
Oliveira, Fernando Eduardo Freitas Lima, Tânia Correia, Alexandra Silva, Ana Margarida Soares, Cristina Morais, Simone Weißelberg, Samira Vilanova, Manuel Rohde, Holger Cerca, Nuno |
author_role |
author |
author2 |
Lima, Tânia Correia, Alexandra Silva, Ana Margarida Soares, Cristina Morais, Simone Weißelberg, Samira Vilanova, Manuel Rohde, Holger Cerca, Nuno |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Fernando Eduardo Freitas Lima, Tânia Correia, Alexandra Silva, Ana Margarida Soares, Cristina Morais, Simone Weißelberg, Samira Vilanova, Manuel Rohde, Holger Cerca, Nuno |
dc.subject.por.fl_str_mv |
Biofilms Virulence Iron Siderophores Mutagenesis Staphylococcus epidermidis Science & Technology |
topic |
Biofilms Virulence Iron Siderophores Mutagenesis Staphylococcus epidermidis Science & Technology |
description |
Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-24 2021-12-24T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75827 |
url |
http://hdl.handle.net/1822/75827 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, Fernando E.; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, Samira; Vilanova, Manuel; Rohde, Holger; Cerca, Nuno, Siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of Staphylococcus epidermidis within the host. Frontiers in Medicine, 8(799227), 2021 2296-858X 10.3389/fmed.2021.799227 https://www.frontiersin.org/articles/10.3389/fmed.2021.799227/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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