Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/62018 |
Resumo: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid NanoparticlestacrinenanoparticlesSH-SY5YMAPAlzheimer’s diseasedrug delivery© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Tacrine (TAC) was the first FDA approved drug for the treatment of Alzheimer’s disease, resulting in increased memory and enhanced cognitive symptoms in patients. However, long-term therapy presents poor patient compliance associated with undesired side effects such as nausea, vomiting and hepatoxicity. To improve its therapeutic efficacy and decrease toxicity, the use of nanoparticles could be applied as a possible solution to delivery TAC. In this context, a project has been designed to develop a new nanostructured lipid carrier (NLC) as a delivery system for TAC and conjugate TAC and model amphipathic peptide (MAP) to decrease TAC limitations. Different formulations loaded with TAC and TAC + MAP were prepared using a combination of Compritol 888 ATO as the solid lipid and Transcutol HP as the liquid lipid component. Physical characterization was evaluated in terms of particle size, surface charge, encapsulation efficiency and in vitro drug release studies. Particle size distributions within the nanometer range were obtained with encapsulation efficiencies of 72.4% for the TAC and 85.6% for the TAC + MAP conjugate. Furthermore, cytotoxicity of all NLC formulations was determined against neuroblastoma cell line SH-SY5Y. The optimized TAC delivery system revealed low toxicity suggesting this could be a potential carrier system to deliver TAC. However, TAC + MAP conjugated even encapsulated in the NLC system demonstrated toxicity against the SH-SY5Y cell line.This research was funded by FCT and FEDER (European Union), grant number IF/00092/2014/CP1255/CT0004. This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional through the COMPETE 2020—Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia, in a framework of CINTESIS, R&D Unit (reference UIDB/4255/2020) and iMed.ULisboa (UIDB/04138/2020 and UIDP/04138/2020). SS thanks FCT for supporting this article with her PhD Grant (PD/BD/135456/2017) and PhD Programme in Medicines and Pharmaceutical Innovation (i3DU) from the University of Lisbon (Portugal) and the University of Porto (Portugal) for all support.MDPIRepositório da Universidade de LisboaSilva, SaraMarto, JoanaGonçalves, LídiaAlmeida, António JoséVale, Nuno2024-01-22T17:54:21Z2020-10-212023-02-27T16:26:42Z2020-10-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/62018engSilva S, Marto J, Gonçalves L, Almeida AJ, Vale N. Formulation, characterization and evaluation against sh-sy5y cells of new tacrine and tacrine-map loaded with lipid nanoparticles. Nanomaterials [Internet]. 21 de outubro de 2020;10(10):2089. Disponível em: https://www.mdpi.com/2079-4991/10/10/20892079-4991cv-prod-211892210.3390/nano10102089info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-29T01:19:36Zoai:repositorio.ul.pt:10451/62018Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:58:29.298287Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| title |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| spellingShingle |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles Silva, Sara tacrine nanoparticles SH-SY5Y MAP Alzheimer’s disease drug delivery |
| title_short |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| title_full |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| title_fullStr |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| title_full_unstemmed |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| title_sort |
Formulation, Characterization and Evaluation against SH-SY5Y Cells of New Tacrine and Tacrine-MAP Loaded with Lipid Nanoparticles |
| author |
Silva, Sara |
| author_facet |
Silva, Sara Marto, Joana Gonçalves, Lídia Almeida, António José Vale, Nuno |
| author_role |
author |
| author2 |
Marto, Joana Gonçalves, Lídia Almeida, António José Vale, Nuno |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Silva, Sara Marto, Joana Gonçalves, Lídia Almeida, António José Vale, Nuno |
| dc.subject.por.fl_str_mv |
tacrine nanoparticles SH-SY5Y MAP Alzheimer’s disease drug delivery |
| topic |
tacrine nanoparticles SH-SY5Y MAP Alzheimer’s disease drug delivery |
| description |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-21 2020-10-21T00:00:00Z 2023-02-27T16:26:42Z 2024-01-22T17:54:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/62018 |
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http://hdl.handle.net/10451/62018 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Silva S, Marto J, Gonçalves L, Almeida AJ, Vale N. Formulation, characterization and evaluation against sh-sy5y cells of new tacrine and tacrine-map loaded with lipid nanoparticles. Nanomaterials [Internet]. 21 de outubro de 2020;10(10):2089. Disponível em: https://www.mdpi.com/2079-4991/10/10/2089 2079-4991 cv-prod-2118922 10.3390/nano10102089 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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