Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80877 |
Resumo: | The agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) as sole substrate, B. thailandensis E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount produced in the standard medium, which contains glycerol, peptone, and meat extract. When the CSL medium was supplemented with OMW (10% (v/v)), rhamnolipid production further increased up to 253 mg/L in flasks and 269 mg/L in a bioreactor. Rhamnolipids produced in CSL+OMW medium reduced the surface tension up to 27.1 mN/m, with a critical micelle concentration of 51 mg/L, better than the values obtained with the standard medium (28.9 mN/m and 58 mg/L, respectively). However, rhamnolipids produced in CSL+OMW medium displayed a weak emulsifying activity when compared to those produced in the other media. Whereas di-rhamnolipid congeners represented between 90 and 95% of rhamnolipids produced by B. thailandensis E264 in CSL and the standard medium, the relative abundance of mono-rhamnolipids increased up to 55% in the culture medium containing OMW. The difference in the rhamnolipid congeners produced in each medium explains their different surface-active properties. To the best of our knowledge, this is the first report of rhamnolipid production by B. thailandensis using a culture medium containing agro-industrial by-products as sole ingredients. Furthermore, rhamnolipids produced in the different media recovered around 60% of crude oil from contaminated sand, demonstrating its potential application in the petroleum industry and bioremediation. |
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Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residuesBioremediationBiosurfactantCircular economyCorn steep liquorEmulsificationOlive mill wastewaterScience & TechnologyThe agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) as sole substrate, B. thailandensis E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount produced in the standard medium, which contains glycerol, peptone, and meat extract. When the CSL medium was supplemented with OMW (10% (v/v)), rhamnolipid production further increased up to 253 mg/L in flasks and 269 mg/L in a bioreactor. Rhamnolipids produced in CSL+OMW medium reduced the surface tension up to 27.1 mN/m, with a critical micelle concentration of 51 mg/L, better than the values obtained with the standard medium (28.9 mN/m and 58 mg/L, respectively). However, rhamnolipids produced in CSL+OMW medium displayed a weak emulsifying activity when compared to those produced in the other media. Whereas di-rhamnolipid congeners represented between 90 and 95% of rhamnolipids produced by B. thailandensis E264 in CSL and the standard medium, the relative abundance of mono-rhamnolipids increased up to 55% in the culture medium containing OMW. The difference in the rhamnolipid congeners produced in each medium explains their different surface-active properties. To the best of our knowledge, this is the first report of rhamnolipid production by B. thailandensis using a culture medium containing agro-industrial by-products as sole ingredients. Furthermore, rhamnolipids produced in the different media recovered around 60% of crude oil from contaminated sand, demonstrating its potential application in the petroleum industry and bioremediation.This study was funded by PARTEX Oil and Gas, the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020, and the National Science Centre (Poland), under the scope of the project 2020/37/B/NZ9/01519.info:eu-repo/semantics/publishedVersionSpringerUniversidade do MinhoCorreia, JéssicaGudiña, Eduardo JoséLazar, ZbigniewJanek, TomaszTeixeira, J. A.2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80877engCorreia, J., Gudiña, E.J., Lazar, Z. et al. Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues. Appl Microbiol Biotechnol 106, 7477–7489 (2022). https://doi.org/10.1007/s00253-022-12225-10175-759810.1007/s00253-022-12225-136222896https://link.springer.com/article/10.1007/s00253-022-12225-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:58Zoai:repositorium.sdum.uminho.pt:1822/80877Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:17:49.266834Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
title |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
spellingShingle |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues Correia, Jéssica Bioremediation Biosurfactant Circular economy Corn steep liquor Emulsification Olive mill wastewater Science & Technology |
title_short |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
title_full |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
title_fullStr |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
title_full_unstemmed |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
title_sort |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
author |
Correia, Jéssica |
author_facet |
Correia, Jéssica Gudiña, Eduardo José Lazar, Zbigniew Janek, Tomasz Teixeira, J. A. |
author_role |
author |
author2 |
Gudiña, Eduardo José Lazar, Zbigniew Janek, Tomasz Teixeira, J. A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Correia, Jéssica Gudiña, Eduardo José Lazar, Zbigniew Janek, Tomasz Teixeira, J. A. |
dc.subject.por.fl_str_mv |
Bioremediation Biosurfactant Circular economy Corn steep liquor Emulsification Olive mill wastewater Science & Technology |
topic |
Bioremediation Biosurfactant Circular economy Corn steep liquor Emulsification Olive mill wastewater Science & Technology |
description |
The agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) as sole substrate, B. thailandensis E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount produced in the standard medium, which contains glycerol, peptone, and meat extract. When the CSL medium was supplemented with OMW (10% (v/v)), rhamnolipid production further increased up to 253 mg/L in flasks and 269 mg/L in a bioreactor. Rhamnolipids produced in CSL+OMW medium reduced the surface tension up to 27.1 mN/m, with a critical micelle concentration of 51 mg/L, better than the values obtained with the standard medium (28.9 mN/m and 58 mg/L, respectively). However, rhamnolipids produced in CSL+OMW medium displayed a weak emulsifying activity when compared to those produced in the other media. Whereas di-rhamnolipid congeners represented between 90 and 95% of rhamnolipids produced by B. thailandensis E264 in CSL and the standard medium, the relative abundance of mono-rhamnolipids increased up to 55% in the culture medium containing OMW. The difference in the rhamnolipid congeners produced in each medium explains their different surface-active properties. To the best of our knowledge, this is the first report of rhamnolipid production by B. thailandensis using a culture medium containing agro-industrial by-products as sole ingredients. Furthermore, rhamnolipids produced in the different media recovered around 60% of crude oil from contaminated sand, demonstrating its potential application in the petroleum industry and bioremediation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10 2022-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80877 |
url |
https://hdl.handle.net/1822/80877 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Correia, J., Gudiña, E.J., Lazar, Z. et al. Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues. Appl Microbiol Biotechnol 106, 7477–7489 (2022). https://doi.org/10.1007/s00253-022-12225-1 0175-7598 10.1007/s00253-022-12225-1 36222896 https://link.springer.com/article/10.1007/s00253-022-12225-1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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