WNK2 inhibits autophagic flux in human glioblastoma cell line
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/64447 |
Resumo: | The following are available online at http://www.mdpi.com/2073-4409/9/2/485/s1, Figure S1: Validation of WNK2 overexpression by RT-PCR. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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WNK2 inhibits autophagic flux in human glioblastoma cell lineWNK2glioblastoma cell lineautophagyinhibitionautophagic fluxScience & TechnologyThe following are available online at http://www.mdpi.com/2073-4409/9/2/485/s1, Figure S1: Validation of WNK2 overexpression by RT-PCR.Autophagy is a cell-survival pathway with dual role in tumorigenesis, promoting either tumor survival or tumor death. <i>WNK2</i> gene, a member of the WNK (with no lysine (K)) subfamily, acts as a tumor suppressor gene in gliomas, regulating cell migration and invasion; however, its role in autophagy process is poorly explored. The <i>WNK2</i>-methylated human glioblastoma cell line A172 WT (wild type) was compared to transfected clones A172 EV (empty vector), and A172 WNK2 (WNK2 overexpression) for the evaluation of autophagy using an inhibitor (bafilomycin A1—baf A1) and an inducer (everolimus) of autophagic flux. Western blot and immunofluorescence approaches were used to monitor autophagic markers, LC3A/B and SQSTM1/p62. A172 WNK2 cells presented a significant decrease in LC3B and p62 protein levels, and in LC3A/B ratio when compared with control cells, after treatment with baf A1 + everolimus, suggesting that <i>WNK2</i> overexpression inhibits the autophagic flux in gliomas. The mTOR pathway was also evaluated under the same conditions, and the observed results suggest that the inhibition of autophagy mediated by WNK2 occurs through a mTOR-independent pathway. In conclusion, the evaluation of the autophagic process demonstrated that WNK2 inhibits the autophagic flux in glioblastoma cell line.This project was supported by the Barretos Cancer Hospital Internal Research Funds (PAIP) to Rui Manuel Reis and by the Public Ministry of Labor Campinas (Research, Prevention, and Education of Occupational Cancer Project), Campinas, Brazil. Ana Laura Vieira Alves is the recipient of a FAPESP master fellowship (2016/18907-0).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoAlves, Ana Laura VieiraCosta, Angela MargaridaMartinho, Olgada Silva, Vinicius DuvalJordan, PeterSilva, Viviane Aline OliveiraReis, R. M.2020-02-202020-02-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64447engAlves, A.L.V.; Costa, A.M.; Martinho, O.; da Silva, V.D.; Jordan, P.; Silva, V.A.O.; Reis, R.M. WNK2 Inhibits Autophagic Flux in Human Glioblastoma Cell Line. Cells 2020, 9, 485.2073-440910.3390/cells902048532093151https://www.mdpi.com/2073-4409/9/2/485info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:01Zoai:repositorium.sdum.uminho.pt:1822/64447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:20.526562Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| title |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| spellingShingle |
WNK2 inhibits autophagic flux in human glioblastoma cell line Alves, Ana Laura Vieira WNK2 glioblastoma cell line autophagy inhibition autophagic flux Science & Technology |
| title_short |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| title_full |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| title_fullStr |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| title_full_unstemmed |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| title_sort |
WNK2 inhibits autophagic flux in human glioblastoma cell line |
| author |
Alves, Ana Laura Vieira |
| author_facet |
Alves, Ana Laura Vieira Costa, Angela Margarida Martinho, Olga da Silva, Vinicius Duval Jordan, Peter Silva, Viviane Aline Oliveira Reis, R. M. |
| author_role |
author |
| author2 |
Costa, Angela Margarida Martinho, Olga da Silva, Vinicius Duval Jordan, Peter Silva, Viviane Aline Oliveira Reis, R. M. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Alves, Ana Laura Vieira Costa, Angela Margarida Martinho, Olga da Silva, Vinicius Duval Jordan, Peter Silva, Viviane Aline Oliveira Reis, R. M. |
| dc.subject.por.fl_str_mv |
WNK2 glioblastoma cell line autophagy inhibition autophagic flux Science & Technology |
| topic |
WNK2 glioblastoma cell line autophagy inhibition autophagic flux Science & Technology |
| description |
The following are available online at http://www.mdpi.com/2073-4409/9/2/485/s1, Figure S1: Validation of WNK2 overexpression by RT-PCR. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-02-20 2020-02-20T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64447 |
| url |
http://hdl.handle.net/1822/64447 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Alves, A.L.V.; Costa, A.M.; Martinho, O.; da Silva, V.D.; Jordan, P.; Silva, V.A.O.; Reis, R.M. WNK2 Inhibits Autophagic Flux in Human Glioblastoma Cell Line. Cells 2020, 9, 485. 2073-4409 10.3390/cells9020485 32093151 https://www.mdpi.com/2073-4409/9/2/485 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132666048544768 |