Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108850 https://doi.org/10.1371/journal.pone.0163435 |
Resumo: | Prevalence of pneumococcal serotypes in carriage and disease has been described but absolute serotype colonisation densities have not been reported. 515 paediatric nasal swab DNA extracts were subjected to lytA qPCR and molecular serotyping by microarray. Absolute serotype densities were derived from total pneumococcal density (qPCR cycle threshold and standard curve) and relative abundance (microarray) and varied widely. Compared to all serotype densities observed, the strongest evidence of differences was seen for serotypes 21 and 35B (higher) and 3, 38 and non-typeables (lower) (p<0.05) with a similar hierarchy when only a single serotype carriage was assessed. There was no evidence of any overall density differences between children with single or multiple serotypes detected but serotypes with mid-range densities were more prevalent. The hierarchy of distinct pneumococcal serotype carriage densities described here for the first time, may help explain the dynamics of transmission between children. |
id |
RCAP_44ea756c929c57e4279c1338f5f170cc |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/108850 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different DensitiesPrevalence of pneumococcal serotypes in carriage and disease has been described but absolute serotype colonisation densities have not been reported. 515 paediatric nasal swab DNA extracts were subjected to lytA qPCR and molecular serotyping by microarray. Absolute serotype densities were derived from total pneumococcal density (qPCR cycle threshold and standard curve) and relative abundance (microarray) and varied widely. Compared to all serotype densities observed, the strongest evidence of differences was seen for serotypes 21 and 35B (higher) and 3, 38 and non-typeables (lower) (p<0.05) with a similar hierarchy when only a single serotype carriage was assessed. There was no evidence of any overall density differences between children with single or multiple serotypes detected but serotypes with mid-range densities were more prevalent. The hierarchy of distinct pneumococcal serotype carriage densities described here for the first time, may help explain the dynamics of transmission between children.Public Library of Science2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108850http://hdl.handle.net/10316/108850https://doi.org/10.1371/journal.pone.0163435eng1932-6203Rodrigues, FernandaDanon, LeonMorales-Aza, BegoniaSikora, PaulinaThors, ValtyrFerreira, MurielGould, KatherineHinds, JasonFinn, Adaminfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-21T08:51:50Zoai:estudogeral.uc.pt:10316/108850Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:05.602128Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
title |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
spellingShingle |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities Rodrigues, Fernanda |
title_short |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
title_full |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
title_fullStr |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
title_full_unstemmed |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
title_sort |
Pneumococcal Serotypes Colonise the Nasopharynx in Children at Different Densities |
author |
Rodrigues, Fernanda |
author_facet |
Rodrigues, Fernanda Danon, Leon Morales-Aza, Begonia Sikora, Paulina Thors, Valtyr Ferreira, Muriel Gould, Katherine Hinds, Jason Finn, Adam |
author_role |
author |
author2 |
Danon, Leon Morales-Aza, Begonia Sikora, Paulina Thors, Valtyr Ferreira, Muriel Gould, Katherine Hinds, Jason Finn, Adam |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Fernanda Danon, Leon Morales-Aza, Begonia Sikora, Paulina Thors, Valtyr Ferreira, Muriel Gould, Katherine Hinds, Jason Finn, Adam |
description |
Prevalence of pneumococcal serotypes in carriage and disease has been described but absolute serotype colonisation densities have not been reported. 515 paediatric nasal swab DNA extracts were subjected to lytA qPCR and molecular serotyping by microarray. Absolute serotype densities were derived from total pneumococcal density (qPCR cycle threshold and standard curve) and relative abundance (microarray) and varied widely. Compared to all serotype densities observed, the strongest evidence of differences was seen for serotypes 21 and 35B (higher) and 3, 38 and non-typeables (lower) (p<0.05) with a similar hierarchy when only a single serotype carriage was assessed. There was no evidence of any overall density differences between children with single or multiple serotypes detected but serotypes with mid-range densities were more prevalent. The hierarchy of distinct pneumococcal serotype carriage densities described here for the first time, may help explain the dynamics of transmission between children. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108850 http://hdl.handle.net/10316/108850 https://doi.org/10.1371/journal.pone.0163435 |
url |
http://hdl.handle.net/10316/108850 https://doi.org/10.1371/journal.pone.0163435 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134134350643200 |