Cardoon hydrolysate detoxification by activated carbon or membranes system for bioethanol production

• Autor(a) principal: Tavares, Ana P. M.
• Data de Publicação: 2022
• Outros Autores: Gonçalves, Matthew J. A., Brás, Teresa, Pesce, Gaetano R., Xavier, Ana M. R. B., Fernandes, Maria C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/35938
• topic: Bioethanol; Cardoon hemicellulosic hydrolysate detoxification; Activated carbon adsorption; Membrane nanofiltration; Microbial fermentation; Scheffersomyces stipites; Modified Escherichia coli

Advanced biofuels incorporation into the transportation sector, particularly cellulosic bioethanol, is crucial for attaining carbon neutrality by 2050, contributing to climate changes mitigation and wastes minimization. The world needs biofuel to be commercially available to tackle the socioeconomic challenges coming from the continued use of fossil fuels. Cynara cardunculus (cardoon) is a cheap lignocellulosic raw biomass that easily grows in Mediterraneous soils and is a potential renewable resource for a biorefinery. This work aimed to study the bioethanol production from cardoon hemicellulosic hydrolysates, which originated from dilute sulfuric acid hydrolysis pretreatment. A detoxification step to remove released microbial fermentative inhibitors was evaluated by using both activated carbon adsorption and a nanofiltration membrane system. The Scheffersomyces stipitis CBS5773 yeast and the modified Escherichia coli MS04 fermentation performances at different experimental conditions were compared. The promising results with E. coli, using detoxified cardoon by membrane nanofiltration, led to a bioethanol volumetric productivity of 0.30 g L1 h1, with a conversion efficiency of 94.5%. Regarding the S. stipitis, in similar fermentation conditions, volumetric productivity of 0.091 g L1 h1 with a conversion efficiency of 64.9% was obtained. Concluding, the production of bioethanol through detoxification of hemicellulosic cardoon hydrolysate presents a suitable alternative for the production of second-generation bioethanol, especially using the modified E. coli.