Probing CHO cells metabolism using metabolomics and fluxomics tools

Detalhes bibliográficos
Autor(a) principal: Duarte, Tiago Martins da Costa
Data de Publicação: 2016
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/55746
Resumo: Chinese hamster ovary (CHO) cells are preferred mammalian hosts for industrial production of therapeutic glycoproteins such as monoclonal antibodies (mAbs), used to treat cancer and immunological disorders. In these cells, the glutamine synthetase (GS) expression system has been widely used for efficient selection of high-yielding clones. However, fundamental knowledge on the metabolic behavior of GS-CHO cells in culture and its impact on product yields have not been yet systematized. The overall objective of this thesis was to comprehensively analyse the metabolome and fluxome of CHO cells exploring how the metabolism is affected by clonal variability and culture conditions. This information is then used to generate hypotheses on how metabolic wiring impacts mAb production.(...)
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spelling Probing CHO cells metabolism using metabolomics and fluxomics toolsMetabolomicsFluxomicsTechnological and Engineering SciencesChinese hamster ovary (CHO) cells are preferred mammalian hosts for industrial production of therapeutic glycoproteins such as monoclonal antibodies (mAbs), used to treat cancer and immunological disorders. In these cells, the glutamine synthetase (GS) expression system has been widely used for efficient selection of high-yielding clones. However, fundamental knowledge on the metabolic behavior of GS-CHO cells in culture and its impact on product yields have not been yet systematized. The overall objective of this thesis was to comprehensively analyse the metabolome and fluxome of CHO cells exploring how the metabolism is affected by clonal variability and culture conditions. This information is then used to generate hypotheses on how metabolic wiring impacts mAb production.(...)PTDC/BBB-BSS/0518/2012Alves, Paula MarquesTeixeira, Ana Margarida PalmaRUNDuarte, Tiago Martins da Costa2019-05-31T00:31:01Z20162016-062016-01-01T00:00:00Zdoctoral thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10362/55746enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:36:11Zoai:run.unl.pt:10362/55746Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:36:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Probing CHO cells metabolism using metabolomics and fluxomics tools
title Probing CHO cells metabolism using metabolomics and fluxomics tools
spellingShingle Probing CHO cells metabolism using metabolomics and fluxomics tools
Duarte, Tiago Martins da Costa
Metabolomics
Fluxomics
Technological and Engineering Sciences
title_short Probing CHO cells metabolism using metabolomics and fluxomics tools
title_full Probing CHO cells metabolism using metabolomics and fluxomics tools
title_fullStr Probing CHO cells metabolism using metabolomics and fluxomics tools
title_full_unstemmed Probing CHO cells metabolism using metabolomics and fluxomics tools
title_sort Probing CHO cells metabolism using metabolomics and fluxomics tools
author Duarte, Tiago Martins da Costa
author_facet Duarte, Tiago Martins da Costa
author_role author
dc.contributor.none.fl_str_mv Alves, Paula Marques
Teixeira, Ana Margarida Palma
RUN
dc.contributor.author.fl_str_mv Duarte, Tiago Martins da Costa
dc.subject.por.fl_str_mv Metabolomics
Fluxomics
Technological and Engineering Sciences
topic Metabolomics
Fluxomics
Technological and Engineering Sciences
description Chinese hamster ovary (CHO) cells are preferred mammalian hosts for industrial production of therapeutic glycoproteins such as monoclonal antibodies (mAbs), used to treat cancer and immunological disorders. In these cells, the glutamine synthetase (GS) expression system has been widely used for efficient selection of high-yielding clones. However, fundamental knowledge on the metabolic behavior of GS-CHO cells in culture and its impact on product yields have not been yet systematized. The overall objective of this thesis was to comprehensively analyse the metabolome and fluxome of CHO cells exploring how the metabolism is affected by clonal variability and culture conditions. This information is then used to generate hypotheses on how metabolic wiring impacts mAb production.(...)
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-06
2016-01-01T00:00:00Z
2019-05-31T00:31:01Z
dc.type.driver.fl_str_mv doctoral thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/55746
url http://hdl.handle.net/10362/55746
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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