Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.7/756 |
Resumo: | This deposit is composed by the main article and supplementary files of the publication. |
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Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cellsBiophysicsCancerCytoskeletonThis deposit is composed by the main article and supplementary files of the publication.Studies of the role of actin in tumour progression have highlighted its key contribution in cell softening associated with cell invasion. Here, using a human breast cell line with conditional Src induction, we demonstrate that cells undergo a stiffening state prior to acquiring malignant features. This state is characterized by the transient accumulation of stress fibres and upregulation of Ena/VASP-like (EVL). EVL, in turn, organizes stress fibres leading to transient cell stiffening, ERK-dependent cell proliferation, as well as enhancement of Src activation and progression towards a fully transformed state. Accordingly, EVL accumulates predominantly in premalignant breast lesions and is required for Src-induced epithelial overgrowth in Drosophila. While cell softening allows for cancer cell invasion, our work reveals that stress fibre-mediated cell stiffening could drive tumour growth during premalignant stages. A careful consideration of the mechanical properties of tumour cells could therefore offer new avenues of exploration when designing cancer-targeting therapies.Bloomington Drosophila Stock Centre; Vienna Drosophila Research Center (VDRC); Developmental Studies Hybridoma Bank (DSHB); Fundação para a Ciência e Tecnologia (FCT) grant: (IF/01031/2012); Laço Grant in breast cancer 2015; Alexander von Humboldt Foundation grant: (Alexander von Humboldt Professorship); Liga Portuguesa contra o Cancro/Pfizer.Nature Publishing GroupARCATavares, SandraVieira, André FilipeTaubenberger, Anna VerenaAraújo, MargaridaMartins, Nuno PimpaoBrás-Pereira, CatarinaPolónia, AntónioHerbig, MaikBarreto, ClaraOtto, OliverCardoso, JoanaPereira-Leal, José B.Guck, JochenParedes, JoanaJanody, Florence2017-05-22T11:38:50Z2017-05-162017-05-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/vnd.ms-excelapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetvideo/quicktimevideo/quicktimevideo/quicktimevideo/quicktimehttp://hdl.handle.net/10400.7/756engTavares, S. et al. Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nat. Commun. 8, 15237 doi: 10.1038/ncomms15237 (2017).10.1038/ncomms15237info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:09Zoai:arca.igc.gulbenkian.pt:10400.7/756Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:58.998054Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| title |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| spellingShingle |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells Tavares, Sandra Biophysics Cancer Cytoskeleton |
| title_short |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| title_full |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| title_fullStr |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| title_full_unstemmed |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| title_sort |
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells |
| author |
Tavares, Sandra |
| author_facet |
Tavares, Sandra Vieira, André Filipe Taubenberger, Anna Verena Araújo, Margarida Martins, Nuno Pimpao Brás-Pereira, Catarina Polónia, António Herbig, Maik Barreto, Clara Otto, Oliver Cardoso, Joana Pereira-Leal, José B. Guck, Jochen Paredes, Joana Janody, Florence |
| author_role |
author |
| author2 |
Vieira, André Filipe Taubenberger, Anna Verena Araújo, Margarida Martins, Nuno Pimpao Brás-Pereira, Catarina Polónia, António Herbig, Maik Barreto, Clara Otto, Oliver Cardoso, Joana Pereira-Leal, José B. Guck, Jochen Paredes, Joana Janody, Florence |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
ARCA |
| dc.contributor.author.fl_str_mv |
Tavares, Sandra Vieira, André Filipe Taubenberger, Anna Verena Araújo, Margarida Martins, Nuno Pimpao Brás-Pereira, Catarina Polónia, António Herbig, Maik Barreto, Clara Otto, Oliver Cardoso, Joana Pereira-Leal, José B. Guck, Jochen Paredes, Joana Janody, Florence |
| dc.subject.por.fl_str_mv |
Biophysics Cancer Cytoskeleton |
| topic |
Biophysics Cancer Cytoskeleton |
| description |
This deposit is composed by the main article and supplementary files of the publication. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-05-22T11:38:50Z 2017-05-16 2017-05-16T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/756 |
| url |
http://hdl.handle.net/10400.7/756 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Tavares, S. et al. Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nat. Commun. 8, 15237 doi: 10.1038/ncomms15237 (2017). 10.1038/ncomms15237 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf application/pdf application/vnd.ms-excel application/vnd.openxmlformats-officedocument.spreadsheetml.sheet video/quicktime video/quicktime video/quicktime video/quicktime |
| dc.publisher.none.fl_str_mv |
Nature Publishing Group |
| publisher.none.fl_str_mv |
Nature Publishing Group |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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