DNA Gel Particles: Particle Preparation and Release Characteristics

Detalhes bibliográficos
Autor(a) principal: Morán, M. Carmen
Data de Publicação: 2007
Outros Autores: Miguel, M. Graça, Lindman, Björn
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/10627
https://doi.org/10.1021/la700672e
Resumo: Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or organic solvent, was demostrated.
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spelling DNA Gel Particles: Particle Preparation and Release CharacteristicsAqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or organic solvent, was demostrated.American Chemical Society2007-06-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10627http://hdl.handle.net/10316/10627https://doi.org/10.1021/la700672eengLangmuir. 23:12 (2007) 6478-64810743-7463Morán, M. CarmenMiguel, M. GraçaLindman, Björninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-02-11T18:17:38Zoai:estudogeral.uc.pt:10316/10627Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:34.563923Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv DNA Gel Particles: Particle Preparation and Release Characteristics
title DNA Gel Particles: Particle Preparation and Release Characteristics
spellingShingle DNA Gel Particles: Particle Preparation and Release Characteristics
Morán, M. Carmen
title_short DNA Gel Particles: Particle Preparation and Release Characteristics
title_full DNA Gel Particles: Particle Preparation and Release Characteristics
title_fullStr DNA Gel Particles: Particle Preparation and Release Characteristics
title_full_unstemmed DNA Gel Particles: Particle Preparation and Release Characteristics
title_sort DNA Gel Particles: Particle Preparation and Release Characteristics
author Morán, M. Carmen
author_facet Morán, M. Carmen
Miguel, M. Graça
Lindman, Björn
author_role author
author2 Miguel, M. Graça
Lindman, Björn
author2_role author
author
dc.contributor.author.fl_str_mv Morán, M. Carmen
Miguel, M. Graça
Lindman, Björn
description Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or organic solvent, was demostrated.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/10627
http://hdl.handle.net/10316/10627
https://doi.org/10.1021/la700672e
url http://hdl.handle.net/10316/10627
https://doi.org/10.1021/la700672e
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Langmuir. 23:12 (2007) 6478-6481
0743-7463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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