Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis

Detalhes bibliográficos
Autor(a) principal: Hollmann, Axel
Data de Publicação: 2012
Outros Autores: Saviello, Mariano, Delfederico, Lucrecia, Saraiva, Tessália Diniz Luerce, Barh, Debmalya, Jain, Neha, Tiwari, Sandeep, Chandra, Sudha, Gupta, Krishnakant, Zambare, Vasudeo, Kumar, Anil, Christopher, Lew, Misra, Amarendra Narayan, Kumavath, Ranjith N., Azevedo, Vasco, Semorile, Liliana, Miyoshi, Anderson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10786
Resumo: © Springer Science+Business Media B.V. 2012
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spelling Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactisInducible heterologous expressionLactobacillus brevisLactococcus lactisS-layer protein© Springer Science+Business Media B.V. 2012Prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xyloseinducible expression system (XIES). SlpA was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of SlpA production revealed that repression of the system with glucose did not require the depletion of xylose from the medium that allows transitory ORF expression. The successful use of XIES to express S-layer proteins in the versatile and generally recognized as safe species L. lactis opens new possibilities for an efficient production and isolation of SlpA S-layer protein for its various applications in biotechnology and importantly as an antigen-carrying vehicle.SpringerRepositório da Universidade de LisboaHollmann, AxelSaviello, MarianoDelfederico, LucreciaSaraiva, Tessália Diniz LuerceBarh, DebmalyaJain, NehaTiwari, SandeepChandra, SudhaGupta, KrishnakantZambare, VasudeoKumar, AnilChristopher, LewMisra, Amarendra NarayanKumavath, Ranjith N.Azevedo, VascoSemorile, LilianaMiyoshi, Anderson2014-03-27T14:30:38Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10786engBiotechnol Lett (2012) 34:1275–12810141-5492http://dx.doi.org/10.1007/s10529-012-0887-6metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:33Zoai:repositorio.ul.pt:10451/10786Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:42.054466Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
title Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
spellingShingle Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
Hollmann, Axel
Inducible heterologous expression
Lactobacillus brevis
Lactococcus lactis
S-layer protein
title_short Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
title_full Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
title_fullStr Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
title_full_unstemmed Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
title_sort Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis
author Hollmann, Axel
author_facet Hollmann, Axel
Saviello, Mariano
Delfederico, Lucrecia
Saraiva, Tessália Diniz Luerce
Barh, Debmalya
Jain, Neha
Tiwari, Sandeep
Chandra, Sudha
Gupta, Krishnakant
Zambare, Vasudeo
Kumar, Anil
Christopher, Lew
Misra, Amarendra Narayan
Kumavath, Ranjith N.
Azevedo, Vasco
Semorile, Liliana
Miyoshi, Anderson
author_role author
author2 Saviello, Mariano
Delfederico, Lucrecia
Saraiva, Tessália Diniz Luerce
Barh, Debmalya
Jain, Neha
Tiwari, Sandeep
Chandra, Sudha
Gupta, Krishnakant
Zambare, Vasudeo
Kumar, Anil
Christopher, Lew
Misra, Amarendra Narayan
Kumavath, Ranjith N.
Azevedo, Vasco
Semorile, Liliana
Miyoshi, Anderson
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Hollmann, Axel
Saviello, Mariano
Delfederico, Lucrecia
Saraiva, Tessália Diniz Luerce
Barh, Debmalya
Jain, Neha
Tiwari, Sandeep
Chandra, Sudha
Gupta, Krishnakant
Zambare, Vasudeo
Kumar, Anil
Christopher, Lew
Misra, Amarendra Narayan
Kumavath, Ranjith N.
Azevedo, Vasco
Semorile, Liliana
Miyoshi, Anderson
dc.subject.por.fl_str_mv Inducible heterologous expression
Lactobacillus brevis
Lactococcus lactis
S-layer protein
topic Inducible heterologous expression
Lactobacillus brevis
Lactococcus lactis
S-layer protein
description © Springer Science+Business Media B.V. 2012
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-27T14:30:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10786
url http://hdl.handle.net/10451/10786
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biotechnol Lett (2012) 34:1275–1281
0141-5492
http://dx.doi.org/10.1007/s10529-012-0887-6
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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