The Use of Feathers from Racing Pigeons for Doping Control Purposes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6329 |
Resumo: | The development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period. |
id |
RCAP_4578bab40c4ac418072d1f7118d2be50 |
---|---|
oai_identifier_str |
oai:repositorio.insa.pt:10400.18/6329 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The Use of Feathers from Racing Pigeons for Doping Control PurposesDoping ControlRacing PigeonsFeathersMethodologies to DetectionThe development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period.This work was supported by the European Union (European Regional Development Fund funds POCI/01/0145/FEDER/007728); and National Funds (Fundação para a Ciência e a Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/MULTI/04378/2013. The study is a result of the project NORTE-01-0145-FEDER-000024, supported by Norte Portugal Regional Operational Programme (NORTE, 2020), under the PORTUGAL 2020 Partnership Agreement (DESignBIOtecHealth— New Technologies for three Health Challenges of Modern Societies: Diabetes, Drug Abuse and Kidney Diseases), through the European Regional Development Fund (ERDF). A.M. wishes to thank the Fundação para a Ciência Tecnologia for grants SFRH/BPD/86898/2012. R.S. acknowledges Fundação para a Ciência e Tecnologia for her Post-doctoral Grant (SFRH/BPD/110201/2015).Oxford University PressRepositório Científico do Instituto Nacional de SaúdeMoreira, Fernando X.Carmo, HelenaMelo, ArmindoAndré, Maria B.Silva, RenataAzevedo, ZéliaBastos, Maria L.de Pinho, Paula G.2019-03-27T12:22:21Z2018-12-152018-12-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6329engJ Anal Toxicol. 2019 May 1;43(4):307-315. doi: 10.1093/jat/bky088. Epub 2018 Dec 15.0146-476010.1093/jat/bky088info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:21Zoai:repositorio.insa.pt:10400.18/6329Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:58.742665Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
title |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
spellingShingle |
The Use of Feathers from Racing Pigeons for Doping Control Purposes Moreira, Fernando X. Doping Control Racing Pigeons Feathers Methodologies to Detection |
title_short |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
title_full |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
title_fullStr |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
title_full_unstemmed |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
title_sort |
The Use of Feathers from Racing Pigeons for Doping Control Purposes |
author |
Moreira, Fernando X. |
author_facet |
Moreira, Fernando X. Carmo, Helena Melo, Armindo André, Maria B. Silva, Renata Azevedo, Zélia Bastos, Maria L. de Pinho, Paula G. |
author_role |
author |
author2 |
Carmo, Helena Melo, Armindo André, Maria B. Silva, Renata Azevedo, Zélia Bastos, Maria L. de Pinho, Paula G. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Moreira, Fernando X. Carmo, Helena Melo, Armindo André, Maria B. Silva, Renata Azevedo, Zélia Bastos, Maria L. de Pinho, Paula G. |
dc.subject.por.fl_str_mv |
Doping Control Racing Pigeons Feathers Methodologies to Detection |
topic |
Doping Control Racing Pigeons Feathers Methodologies to Detection |
description |
The development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-15 2018-12-15T00:00:00Z 2019-03-27T12:22:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6329 |
url |
http://hdl.handle.net/10400.18/6329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
J Anal Toxicol. 2019 May 1;43(4):307-315. doi: 10.1093/jat/bky088. Epub 2018 Dec 15. 0146-4760 10.1093/jat/bky088 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132153049513984 |