Metabolic stability and metabolite profiling of emerging synthetic cathinones

Detalhes bibliográficos
Autor(a) principal: Lopes, Rita P.
Data de Publicação: 2023
Outros Autores: Ferro, Raquel A., Milhazes, Margarida, Figueira, Margarida, Caldeira, Maria João, Antunes, Alexandra M.M., Gaspar, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8770
Resumo: We thank Fundação para a Ciência e a Tecnologia (FCT), Portugal, for financial support through projects UIDB/QUI/00100/2020 and UIDP/00100/2020 (to CQE), LA/P/0056/2020 (to IMS), UIDB/04046/2020 and UIDP/04046/2020 (to BioISIBiosystems & Integrative Sciences Institute), UIDB/04292/2020 and UIDP/04292/2020 (to MARE-Marine and Environmental Sciences Centre). FCT is also acknowledged for the PhD grant 2022.04738. PTDC to RPL. Joint funding from FCT and the COMPETE Program through grant RNEMLISBOA-01-0145-FEDER-022125 funding are also gratefully acknowledged.
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spelling Metabolic stability and metabolite profiling of emerging synthetic cathinones4-CMC3-CMC4-CIC4-MEAP3-CIC4-MDMB4-MNEB4-MDMPWe thank Fundação para a Ciência e a Tecnologia (FCT), Portugal, for financial support through projects UIDB/QUI/00100/2020 and UIDP/00100/2020 (to CQE), LA/P/0056/2020 (to IMS), UIDB/04046/2020 and UIDP/04046/2020 (to BioISIBiosystems & Integrative Sciences Institute), UIDB/04292/2020 and UIDP/04292/2020 (to MARE-Marine and Environmental Sciences Centre). FCT is also acknowledged for the PhD grant 2022.04738. PTDC to RPL. Joint funding from FCT and the COMPETE Program through grant RNEMLISBOA-01-0145-FEDER-022125 funding are also gratefully acknowledged.Synthetic cathinones constitute the second largest groups of new psychoactive substances (NPS), which are especially popular among adolescents and young adults. Due to their potential toxicity, the recreational use of these NPS constitute a serious worldwide public health problem. However, their fast appearance in the market renders the continuous updating of NPS information highly challenging for forensic authorities. The unavailability of pharmacokinetic data for emerging NPS is critical for forensic and clinical verifications. With the ultimate goal of having a proactive approach towards the NPS issue, high resolution mass spectrometry was used in the current work to assess preliminary pharmacokinetic data for 8 selected cathinones: 4 reported substances (4-CIC, 3-CMC, 4-CMC and 4-MEAP) and 4 previously unreported ones (3-CIC, 4-MDMB, 4-MNEB and 4-MDMP) for which the emergence on the NSP market is expected to be eminent, were also included in this study. Based on the calculation of pharmacokinetic parameters, half-life and intrinsic clearance, 4-CMC and 4-MDMB are low and high clearance compounds, respectively, and all the remaining cathinones included in this study are intermediate clearance compounds. This fact anticipates the key role of metabolites as suitable biomarkers to extend detection windows beyond those provided by the parent cathinones. Reduction of the keto group and hydroxylation on the alkyl chains were the common metabolic pathways identified for all cathinones. However, the relative importance of these metabolic transformations is dependent on the cathinone substituents. The glucuronic acid conjugation to metabolites stemming for keto group reduction constituted the sole Phase II transformation identified. To our knowledge, this study constitutes the first metabolite profiling of the already reported synthetic cathinones 4-CIC, 3-CMC and 4-CMC. Noteworthy is the fact that 3-CMC accounts for almost a quarter of the quantity of powders seized during 2020. The analytical methods developed, and the metabolites characterized, are now available to be included in routine screening methods to attest the consumption of the 8 cathinones studied.FrontiersIC-OnlineLopes, Rita P.Ferro, Raquel A.Milhazes, MargaridaFigueira, MargaridaCaldeira, Maria JoãoAntunes, Alexandra M.M.Gaspar, Helena2023-09-08T09:09:26Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8770engLopes RP, Ferro RA, Milhazes M, Figueira M, Caldeira MJ, Antunes AMM and Gaspar H (2023), Metabolic stability and metabolite profiling of emerging synthetic cathinones. Front. Pharmacol. 14:1145140. doi: 10.3389/fphar.2023.11451401663-981210.3389/fphar.2023.1145140info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:58:08Zoai:iconline.ipleiria.pt:10400.8/8770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:25.497359Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolic stability and metabolite profiling of emerging synthetic cathinones
title Metabolic stability and metabolite profiling of emerging synthetic cathinones
spellingShingle Metabolic stability and metabolite profiling of emerging synthetic cathinones
Lopes, Rita P.
4-CMC
3-CMC
4-CIC
4-MEAP
3-CIC
4-MDMB
4-MNEB
4-MDMP
title_short Metabolic stability and metabolite profiling of emerging synthetic cathinones
title_full Metabolic stability and metabolite profiling of emerging synthetic cathinones
title_fullStr Metabolic stability and metabolite profiling of emerging synthetic cathinones
title_full_unstemmed Metabolic stability and metabolite profiling of emerging synthetic cathinones
title_sort Metabolic stability and metabolite profiling of emerging synthetic cathinones
author Lopes, Rita P.
author_facet Lopes, Rita P.
Ferro, Raquel A.
Milhazes, Margarida
Figueira, Margarida
Caldeira, Maria João
Antunes, Alexandra M.M.
Gaspar, Helena
author_role author
author2 Ferro, Raquel A.
Milhazes, Margarida
Figueira, Margarida
Caldeira, Maria João
Antunes, Alexandra M.M.
Gaspar, Helena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Lopes, Rita P.
Ferro, Raquel A.
Milhazes, Margarida
Figueira, Margarida
Caldeira, Maria João
Antunes, Alexandra M.M.
Gaspar, Helena
dc.subject.por.fl_str_mv 4-CMC
3-CMC
4-CIC
4-MEAP
3-CIC
4-MDMB
4-MNEB
4-MDMP
topic 4-CMC
3-CMC
4-CIC
4-MEAP
3-CIC
4-MDMB
4-MNEB
4-MDMP
description We thank Fundação para a Ciência e a Tecnologia (FCT), Portugal, for financial support through projects UIDB/QUI/00100/2020 and UIDP/00100/2020 (to CQE), LA/P/0056/2020 (to IMS), UIDB/04046/2020 and UIDP/04046/2020 (to BioISIBiosystems & Integrative Sciences Institute), UIDB/04292/2020 and UIDP/04292/2020 (to MARE-Marine and Environmental Sciences Centre). FCT is also acknowledged for the PhD grant 2022.04738. PTDC to RPL. Joint funding from FCT and the COMPETE Program through grant RNEMLISBOA-01-0145-FEDER-022125 funding are also gratefully acknowledged.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-08T09:09:26Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8770
url http://hdl.handle.net/10400.8/8770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lopes RP, Ferro RA, Milhazes M, Figueira M, Caldeira MJ, Antunes AMM and Gaspar H (2023), Metabolic stability and metabolite profiling of emerging synthetic cathinones. Front. Pharmacol. 14:1145140. doi: 10.3389/fphar.2023.1145140
1663-9812
10.3389/fphar.2023.1145140
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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