Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86889 |
Resumo: | In this study, the heat resistance of S. aureus in goats raw milk subjected to thermisation temperatures was characterised through tests at various temperatures and modelling the survival curves using the Weibull model, through a two-step and an omnibus approach, which can model a full dataset covering all experimental conditions in one step. The fitting capacity of the secondary models obtained from the two-step approach was reasonable (adj. R2 > 0.639) and both demonstrated the negative linear effects of temperature on (p=0.0004) and (p=0.017). The fitting capacity of the omnibus model was more satisfactory (adj. R2=0.996) and also hinted at the negative linear effect of temperature on (p < 0.0001), with the added advantage that, in this model, random effects can be used to account for the variability in the parameters. Our study estimated the significant inactivation parameters and established a model capable of predicting S. aureus behaviour at various temperatures. This information is useful to create time-temperature tables to reach target log reductions of S. aureus in goats raw milk to be used by artisanal cheesemakers; hence providing an opportunity to increase the microbiological safety of cheeses made from unpasteurised milk. |
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Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperaturesWeibullThermisationHeat treatmentArtisanal cheeseInactivationIn this study, the heat resistance of S. aureus in goats raw milk subjected to thermisation temperatures was characterised through tests at various temperatures and modelling the survival curves using the Weibull model, through a two-step and an omnibus approach, which can model a full dataset covering all experimental conditions in one step. The fitting capacity of the secondary models obtained from the two-step approach was reasonable (adj. R2 > 0.639) and both demonstrated the negative linear effects of temperature on (p=0.0004) and (p=0.017). The fitting capacity of the omnibus model was more satisfactory (adj. R2=0.996) and also hinted at the negative linear effect of temperature on (p < 0.0001), with the added advantage that, in this model, random effects can be used to account for the variability in the parameters. Our study estimated the significant inactivation parameters and established a model capable of predicting S. aureus behaviour at various temperatures. This information is useful to create time-temperature tables to reach target log reductions of S. aureus in goats raw milk to be used by artisanal cheesemakers; hence providing an opportunity to increase the microbiological safety of cheeses made from unpasteurised milk.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). They are also grateful to the EU PRIMA program and the Portuguese Foundation for Science and Technology (FCT) for funding the ArtiSaneFood project (PRIMA/0001/2018). This study was supported by FCT under the scope of the strategic funding of the UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte. B. N. Silva acknowledges the financial support provided by FCT through the Ph.D. grant SFRH/BD/137801/2018.info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoSilva, BeatrizCoelho-Fernandes, SaraTeixeira, J. A.Cadavez, VascoGonzales-Barron, Ursula2023-10-142023-10-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86889engSilva, B. N., Coelho-Fernandes, S., Teixeira, J. A., Cadavez, V., & Gonzales-Barron, U. (2023, October). Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures. Microbial Risk Analysis. Elsevier BV. http://doi.org/10.1016/j.mran.2023.1002792352-352210.1016/j.mran.2023.100279100279https://www.sciencedirect.com/science/article/pii/S2352352223000348info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-02T01:20:49Zoai:repositorium.sdum.uminho.pt:1822/86889Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:39:02.793242Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
title |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
spellingShingle |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures Silva, Beatriz Weibull Thermisation Heat treatment Artisanal cheese Inactivation |
title_short |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
title_full |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
title_fullStr |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
title_full_unstemmed |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
title_sort |
Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures |
author |
Silva, Beatriz |
author_facet |
Silva, Beatriz Coelho-Fernandes, Sara Teixeira, J. A. Cadavez, Vasco Gonzales-Barron, Ursula |
author_role |
author |
author2 |
Coelho-Fernandes, Sara Teixeira, J. A. Cadavez, Vasco Gonzales-Barron, Ursula |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Beatriz Coelho-Fernandes, Sara Teixeira, J. A. Cadavez, Vasco Gonzales-Barron, Ursula |
dc.subject.por.fl_str_mv |
Weibull Thermisation Heat treatment Artisanal cheese Inactivation |
topic |
Weibull Thermisation Heat treatment Artisanal cheese Inactivation |
description |
In this study, the heat resistance of S. aureus in goats raw milk subjected to thermisation temperatures was characterised through tests at various temperatures and modelling the survival curves using the Weibull model, through a two-step and an omnibus approach, which can model a full dataset covering all experimental conditions in one step. The fitting capacity of the secondary models obtained from the two-step approach was reasonable (adj. R2 > 0.639) and both demonstrated the negative linear effects of temperature on (p=0.0004) and (p=0.017). The fitting capacity of the omnibus model was more satisfactory (adj. R2=0.996) and also hinted at the negative linear effect of temperature on (p < 0.0001), with the added advantage that, in this model, random effects can be used to account for the variability in the parameters. Our study estimated the significant inactivation parameters and established a model capable of predicting S. aureus behaviour at various temperatures. This information is useful to create time-temperature tables to reach target log reductions of S. aureus in goats raw milk to be used by artisanal cheesemakers; hence providing an opportunity to increase the microbiological safety of cheeses made from unpasteurised milk. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-14 2023-10-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86889 |
url |
https://hdl.handle.net/1822/86889 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, B. N., Coelho-Fernandes, S., Teixeira, J. A., Cadavez, V., & Gonzales-Barron, U. (2023, October). Modelling the kinetics of Staphylococcus aureus in goat's raw milk under different sub-pasteurisation temperatures. Microbial Risk Analysis. Elsevier BV. http://doi.org/10.1016/j.mran.2023.100279 2352-3522 10.1016/j.mran.2023.100279 100279 https://www.sciencedirect.com/science/article/pii/S2352352223000348 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133650367807488 |