Linear regression for calibration lines revisited: weighting schemes for bioanalytical methods

• Autor(a) principal: Almeida, Anabela Maduro de
• Data de Publicação: 2002
• Outros Autores: Castel-Branco, Maria Margarida, Falcão, Amílcar
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10316/277; https://doi.org/10.1016/S1570-0232(02)00244-1
• topic: regressão linear; métodos bioanalíticos; curvas de calibração

When the assumption of homoscedasticity is not met for analytical data, a simple and effective way to counteract the greater influence of the greater concentrations on the fitted regression line is to use weighted least squares linear regression (WLSLR). The purpose of the present paper is to stress the relevance of weighting schemes for linear regression analysis and to show how this approach can be useful in the bioanalytical field. The steps to be taken in the study of the linear calibration approach are described. The application of weighting schemes was shown by using a high-performance liquid chromatography method for the determination of lamotrigine in biological fluids as a practical example. By using the WLSLR, the accuracy of the analytical method was improved at the lower end of the calibration curve. Bioanalytical methods data analysis was improved by using the WLSLR procedure.