Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis

Detalhes bibliográficos
Autor(a) principal: Ferreira, FJ
Data de Publicação: 2021
Outros Autores: Carvalho, L, Logarinho, E, Bessa, J
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/152527
Resumo: Foxm1 is a master regulator of the cell cycle, contributing to cell proliferation. Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the zebrafish terminally differentiated fast-twitching muscle cells. foxm1 genomic disruption increased myofiber death and clearance. Interestingly, this contributed to non-autonomous satellite cell activation and proliferation. Moreover, we observed that Cas9 expression alone was strongly deleterious to muscle cells. Our report shows that foxm1 modulates a muscle non-autonomous response to myofiber death and highlights underreported toxicity to high expression of Cas9 in vivo.
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spelling Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasisCRISPR/Cas9Foxm1Satellite cellsSkeletal muscleZebrafishFoxm1 is a master regulator of the cell cycle, contributing to cell proliferation. Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the zebrafish terminally differentiated fast-twitching muscle cells. foxm1 genomic disruption increased myofiber death and clearance. Interestingly, this contributed to non-autonomous satellite cell activation and proliferation. Moreover, we observed that Cas9 expression alone was strongly deleterious to muscle cells. Our report shows that foxm1 modulates a muscle non-autonomous response to myofiber death and highlights underreported toxicity to high expression of Cas9 in vivo.MDPI20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/152527eng2073-440910.3390/cells10051241Ferreira, FJCarvalho, LLogarinho, EBessa, Jinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:12:38Zoai:repositorio-aberto.up.pt:10216/152527Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:35:56.902418Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
title Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
spellingShingle Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
Ferreira, FJ
CRISPR/Cas9
Foxm1
Satellite cells
Skeletal muscle
Zebrafish
title_short Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
title_full Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
title_fullStr Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
title_full_unstemmed Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
title_sort Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis
author Ferreira, FJ
author_facet Ferreira, FJ
Carvalho, L
Logarinho, E
Bessa, J
author_role author
author2 Carvalho, L
Logarinho, E
Bessa, J
author2_role author
author
author
dc.contributor.author.fl_str_mv Ferreira, FJ
Carvalho, L
Logarinho, E
Bessa, J
dc.subject.por.fl_str_mv CRISPR/Cas9
Foxm1
Satellite cells
Skeletal muscle
Zebrafish
topic CRISPR/Cas9
Foxm1
Satellite cells
Skeletal muscle
Zebrafish
description Foxm1 is a master regulator of the cell cycle, contributing to cell proliferation. Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the zebrafish terminally differentiated fast-twitching muscle cells. foxm1 genomic disruption increased myofiber death and clearance. Interestingly, this contributed to non-autonomous satellite cell activation and proliferation. Moreover, we observed that Cas9 expression alone was strongly deleterious to muscle cells. Our report shows that foxm1 modulates a muscle non-autonomous response to myofiber death and highlights underreported toxicity to high expression of Cas9 in vivo.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/152527
url https://hdl.handle.net/10216/152527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2073-4409
10.3390/cells10051241
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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