Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration

Detalhes bibliográficos
Autor(a) principal: Brandão, Ana S.
Data de Publicação: 2019
Outros Autores: Bensimon-Brito, Anabela, Lourenço, Raquel, Borbinha, Jorge, Soares, Ana Rosa, Mateus, Rita, Jacinto, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147516
Resumo: Funding This work was funded by Fundaçãõ o para a Ciência e a Tecnologia (FCT; PTDC/BIMMED/0659/2014; SFRH/BD/51990/2012 to A.S.B.; SFRH/BD/131929/2017 to J.B.; PD/BD/106052/2015 to A.R.S.). R.L. was supported by the FCT in the context of a program contract (D.L. no. 57/2016). Zebrafish maintenance was supported by Congento LISBOA-01-0145-FEDER-022170, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund).
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spelling Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regenerationBoneHippo pathwayOsteoblastParacrine signallingRegenerationCell BiologyFunding This work was funded by Fundaçãõ o para a Ciência e a Tecnologia (FCT; PTDC/BIMMED/0659/2014; SFRH/BD/51990/2012 to A.S.B.; SFRH/BD/131929/2017 to J.B.; PD/BD/106052/2015 to A.R.S.). R.L. was supported by the FCT in the context of a program contract (D.L. no. 57/2016). Zebrafish maintenance was supported by Congento LISBOA-01-0145-FEDER-022170, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund).Osteoblast differentiation is a key process for bone homeostasis and repair. Multiple signalling pathways have been associated with osteoblast differentiation, yet much remains unknown on how this process is regulated in vivo Previous studies have proposed that the Hippo pathway transcriptional co-activators YAP and TAZ (also known as YAP1 and WWTR1, respectively) maintain progenitor stemness and inhibit terminal differentiation of osteoblasts, whereas others suggest they potentiate osteoblast differentiation and bone formation. Here, we use zebrafish caudal fin regeneration as a model to clarify how the Hippo pathway regulates de novo bone formation and osteoblast differentiation. We demonstrate that Yap inhibition leads to accumulation of osteoprogenitors and prevents osteoblast differentiation in a cell non-autonomous manner. This effect correlates with a severe impairment of Bmp signalling in osteoblasts, likely by suppressing the expression of the ligand bmp2a in the surrounding mesenchymal cells. Overall, our findings provide a new mechanism of bone formation through the Hippo-Yap pathway, integrating Yap in the signalling cascade that governs osteoprogenitor maintenance and subsequent differentiation during zebrafish caudal fin regeneration.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBrandão, Ana S.Bensimon-Brito, AnabelaLourenço, RaquelBorbinha, JorgeSoares, Ana RosaMateus, RitaJacinto, António2023-01-13T22:11:17Z2019-11-142019-11-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147516eng0021-9533PURE: 15568465https://doi.org/10.1242/jcs.231993info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:40Zoai:run.unl.pt:10362/147516Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:58.109411Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
title Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
spellingShingle Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
Brandão, Ana S.
Bone
Hippo pathway
Osteoblast
Paracrine signalling
Regeneration
Cell Biology
title_short Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
title_full Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
title_fullStr Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
title_full_unstemmed Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
title_sort Yap induces osteoblast differentiation by modulating Bmp signalling during zebrafish caudal fin regeneration
author Brandão, Ana S.
author_facet Brandão, Ana S.
Bensimon-Brito, Anabela
Lourenço, Raquel
Borbinha, Jorge
Soares, Ana Rosa
Mateus, Rita
Jacinto, António
author_role author
author2 Bensimon-Brito, Anabela
Lourenço, Raquel
Borbinha, Jorge
Soares, Ana Rosa
Mateus, Rita
Jacinto, António
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Brandão, Ana S.
Bensimon-Brito, Anabela
Lourenço, Raquel
Borbinha, Jorge
Soares, Ana Rosa
Mateus, Rita
Jacinto, António
dc.subject.por.fl_str_mv Bone
Hippo pathway
Osteoblast
Paracrine signalling
Regeneration
Cell Biology
topic Bone
Hippo pathway
Osteoblast
Paracrine signalling
Regeneration
Cell Biology
description Funding This work was funded by Fundaçãõ o para a Ciência e a Tecnologia (FCT; PTDC/BIMMED/0659/2014; SFRH/BD/51990/2012 to A.S.B.; SFRH/BD/131929/2017 to J.B.; PD/BD/106052/2015 to A.R.S.). R.L. was supported by the FCT in the context of a program contract (D.L. no. 57/2016). Zebrafish maintenance was supported by Congento LISBOA-01-0145-FEDER-022170, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund).
publishDate 2019
dc.date.none.fl_str_mv 2019-11-14
2019-11-14T00:00:00Z
2023-01-13T22:11:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147516
url http://hdl.handle.net/10362/147516
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9533
PURE: 15568465
https://doi.org/10.1242/jcs.231993
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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