Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/16096 |
Resumo: | Tabebuia impetiginosa (Mart. ex DC.) Standl. has been used in traditional medicine for many centuries, being nowadays marketed as dried plant material (inner bark) for infusions, pills, and syrups. The main objective of the present work was to validate its popular use through the bioactivity evaluation of the inner bark (methanolic extract and infusion) and of two different formulations (pills and syrup) also based on the same plant-material. The antioxidant activity was evaluated by in vitro assays testing free radical scavenging activity, reducing power and inhibition of lipid peroxidation in brain homogenates. The cytotoxicity was determined in four human tumor cell lines (MCF-7, NCI-H460, HeLa and HepG2, and also in non-tumor cells (porcine liver primary cells, PLP2)). Furthermore, the sample was chemically characterized regarding free sugars, organic acids, fatty acids, and tocopherols. Syrup and methanolic extract showed the highest antioxidant activity, related to their highest amount of phenolics and flavonoids. Methanolic extract was the only sample showing cytotoxic effects on the tested human tumor cell lines, but none of the samples showed toxicity in PLP2. Glucose and oxalic acid were, respectively, the most abundant sugar and organic acid in the sample. Unsaturated predominated over the saturated fatty acids, due to oleic, linoleic, and linolenic acids expression. α- and γ-Tocopherols were also identified and quantified. Overall, T. impetiginosa might be used in different phytoformulations, taking advantage of its interesting bioactive properties and chemical composition. |
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Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplementsAntioxidantsChemical compositionCytotoxicityMethanolic extract/infusion/dietary supplementsTabebuia impetiginosaTabebuia impetiginosa (Mart. ex DC.) Standl. has been used in traditional medicine for many centuries, being nowadays marketed as dried plant material (inner bark) for infusions, pills, and syrups. The main objective of the present work was to validate its popular use through the bioactivity evaluation of the inner bark (methanolic extract and infusion) and of two different formulations (pills and syrup) also based on the same plant-material. The antioxidant activity was evaluated by in vitro assays testing free radical scavenging activity, reducing power and inhibition of lipid peroxidation in brain homogenates. The cytotoxicity was determined in four human tumor cell lines (MCF-7, NCI-H460, HeLa and HepG2, and also in non-tumor cells (porcine liver primary cells, PLP2)). Furthermore, the sample was chemically characterized regarding free sugars, organic acids, fatty acids, and tocopherols. Syrup and methanolic extract showed the highest antioxidant activity, related to their highest amount of phenolics and flavonoids. Methanolic extract was the only sample showing cytotoxic effects on the tested human tumor cell lines, but none of the samples showed toxicity in PLP2. Glucose and oxalic acid were, respectively, the most abundant sugar and organic acid in the sample. Unsaturated predominated over the saturated fatty acids, due to oleic, linoleic, and linolenic acids expression. α- and γ-Tocopherols were also identified and quantified. Overall, T. impetiginosa might be used in different phytoformulations, taking advantage of its interesting bioactive properties and chemical composition.The authors are grateful to Foundation for Science and Technology (FCT, Portugal) for financial support to the research centre CIMO (strategic project PEst-OE/AGR/UI0690/2011), L. Barros researcher contract under “Programa Compromisso com Ciência–2008” and R.C. Calhelha grant (SFRH/BPD/BPD/68344/2010).Biblioteca Digital do IPBPires, Tânia C.S.P.Dias, Maria InêsCalhelha, Ricardo C.Carvalho, Ana MariaQueiroz, Maria João R.P.Barros, LillianFerreira, Isabel C.F.R.McPhee, Derek J.2018-01-19T10:00:00Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/16096engPires, Tânia C.S.P.; Dias, Maria Inês; Calhelha, Ricardo C.; Carvalho, Ana Maria; Queiroz, Maria João R.P.; Barros, Lillian; Ferreira, Isabel C.F.R.; McPhee, Derek J. (2015). Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: A comparison between extracts and dietary supplements. Molecules. ISSN 1420-3049. 20, p. 22863-228711420-304910.3390/molecules201219885info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:37:06Zoai:bibliotecadigital.ipb.pt:10198/16096Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:05:22.387714Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
title |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
spellingShingle |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements Pires, Tânia C.S.P. Antioxidants Chemical composition Cytotoxicity Methanolic extract/infusion/dietary supplements Tabebuia impetiginosa |
title_short |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
title_full |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
title_fullStr |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
title_full_unstemmed |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
title_sort |
Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: a comparison between extracts and dietary supplements |
author |
Pires, Tânia C.S.P. |
author_facet |
Pires, Tânia C.S.P. Dias, Maria Inês Calhelha, Ricardo C. Carvalho, Ana Maria Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. McPhee, Derek J. |
author_role |
author |
author2 |
Dias, Maria Inês Calhelha, Ricardo C. Carvalho, Ana Maria Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. McPhee, Derek J. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Pires, Tânia C.S.P. Dias, Maria Inês Calhelha, Ricardo C. Carvalho, Ana Maria Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. McPhee, Derek J. |
dc.subject.por.fl_str_mv |
Antioxidants Chemical composition Cytotoxicity Methanolic extract/infusion/dietary supplements Tabebuia impetiginosa |
topic |
Antioxidants Chemical composition Cytotoxicity Methanolic extract/infusion/dietary supplements Tabebuia impetiginosa |
description |
Tabebuia impetiginosa (Mart. ex DC.) Standl. has been used in traditional medicine for many centuries, being nowadays marketed as dried plant material (inner bark) for infusions, pills, and syrups. The main objective of the present work was to validate its popular use through the bioactivity evaluation of the inner bark (methanolic extract and infusion) and of two different formulations (pills and syrup) also based on the same plant-material. The antioxidant activity was evaluated by in vitro assays testing free radical scavenging activity, reducing power and inhibition of lipid peroxidation in brain homogenates. The cytotoxicity was determined in four human tumor cell lines (MCF-7, NCI-H460, HeLa and HepG2, and also in non-tumor cells (porcine liver primary cells, PLP2)). Furthermore, the sample was chemically characterized regarding free sugars, organic acids, fatty acids, and tocopherols. Syrup and methanolic extract showed the highest antioxidant activity, related to their highest amount of phenolics and flavonoids. Methanolic extract was the only sample showing cytotoxic effects on the tested human tumor cell lines, but none of the samples showed toxicity in PLP2. Glucose and oxalic acid were, respectively, the most abundant sugar and organic acid in the sample. Unsaturated predominated over the saturated fatty acids, due to oleic, linoleic, and linolenic acids expression. α- and γ-Tocopherols were also identified and quantified. Overall, T. impetiginosa might be used in different phytoformulations, taking advantage of its interesting bioactive properties and chemical composition. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2018-01-19T10:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/16096 |
url |
http://hdl.handle.net/10198/16096 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pires, Tânia C.S.P.; Dias, Maria Inês; Calhelha, Ricardo C.; Carvalho, Ana Maria; Queiroz, Maria João R.P.; Barros, Lillian; Ferreira, Isabel C.F.R.; McPhee, Derek J. (2015). Bioactive properties of tabebuia impetiginosa-based phytopreparations and phytoformulations: A comparison between extracts and dietary supplements. Molecules. ISSN 1420-3049. 20, p. 22863-22871 1420-3049 10.3390/molecules201219885 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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