Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix

Detalhes bibliográficos
Autor(a) principal: Gomes, F. I.
Data de Publicação: 2011
Outros Autores: Cerca, Nuno, Teixeira, P., Azeredo, Joana, Oliveira, Rosário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/16616
Resumo: Staphylococcus epidermidis is the most frequent cause of nosocomial sepsis and catheter-related infections in which biofilm formation is considered to be one of the main virulence mechanisms. Moreover, their increased resistance to conventional antibiotic therapy enhances the need to develop new therapeutical agents. Farnesol, a natural sesquiterpenoid present in many essential oils, has been described as impairing bacterial growth. The aim of this study was to evaluate the effect of farnesol on the structure and composition of biofilm matrix of S. epidermidis. Biofilms formed in the presence of farnesol (300 μM) contained less biomass, and displayed notable changes in the composition of the biofilm matrix. Changes in the spacial structure were also verified by confocal scanning laser microscopy (CSLM). The results obtained by the quantification of extracellular polymers and by wheat germ agglutinin (WGA) fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-d-glucosamine support the hypothesis that farnesol causes disruption of the cytoplasmic membrane and consequently release of cellular content.
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spelling Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrixScience & TechnologyStaphylococcus epidermidis is the most frequent cause of nosocomial sepsis and catheter-related infections in which biofilm formation is considered to be one of the main virulence mechanisms. Moreover, their increased resistance to conventional antibiotic therapy enhances the need to develop new therapeutical agents. Farnesol, a natural sesquiterpenoid present in many essential oils, has been described as impairing bacterial growth. The aim of this study was to evaluate the effect of farnesol on the structure and composition of biofilm matrix of S. epidermidis. Biofilms formed in the presence of farnesol (300 μM) contained less biomass, and displayed notable changes in the composition of the biofilm matrix. Changes in the spacial structure were also verified by confocal scanning laser microscopy (CSLM). The results obtained by the quantification of extracellular polymers and by wheat germ agglutinin (WGA) fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-d-glucosamine support the hypothesis that farnesol causes disruption of the cytoplasmic membrane and consequently release of cellular content.Fernanda Gomes and Pilar Teixeira fully acknowledge the financial support of Fundacao para a Ciencia e Tecnologia (FCT) through the grants SFRH/BD/32126/2006 and SFRH/BPD/26803/2006, respectively.SpringerUniversidade do MinhoGomes, F. I.Cerca, NunoTeixeira, P.Azeredo, JoanaOliveira, Rosário2011-07-012011-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16616eng0343-865110.1007/s00284-011-9984-321800262http://www.springerlink.com/content/a21558x611481v25/fulltext.pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:09Zoai:repositorium.sdum.uminho.pt:1822/16616Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:09.729326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
title Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
spellingShingle Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
Gomes, F. I.
Science & Technology
title_short Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
title_full Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
title_fullStr Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
title_full_unstemmed Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
title_sort Effect of farnesol on structure and composition of staphylococcus epidermidis biofilm matrix
author Gomes, F. I.
author_facet Gomes, F. I.
Cerca, Nuno
Teixeira, P.
Azeredo, Joana
Oliveira, Rosário
author_role author
author2 Cerca, Nuno
Teixeira, P.
Azeredo, Joana
Oliveira, Rosário
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gomes, F. I.
Cerca, Nuno
Teixeira, P.
Azeredo, Joana
Oliveira, Rosário
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Staphylococcus epidermidis is the most frequent cause of nosocomial sepsis and catheter-related infections in which biofilm formation is considered to be one of the main virulence mechanisms. Moreover, their increased resistance to conventional antibiotic therapy enhances the need to develop new therapeutical agents. Farnesol, a natural sesquiterpenoid present in many essential oils, has been described as impairing bacterial growth. The aim of this study was to evaluate the effect of farnesol on the structure and composition of biofilm matrix of S. epidermidis. Biofilms formed in the presence of farnesol (300 μM) contained less biomass, and displayed notable changes in the composition of the biofilm matrix. Changes in the spacial structure were also verified by confocal scanning laser microscopy (CSLM). The results obtained by the quantification of extracellular polymers and by wheat germ agglutinin (WGA) fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-d-glucosamine support the hypothesis that farnesol causes disruption of the cytoplasmic membrane and consequently release of cellular content.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-01
2011-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/16616
url http://hdl.handle.net/1822/16616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0343-8651
10.1007/s00284-011-9984-3
21800262
http://www.springerlink.com/content/a21558x611481v25/fulltext.pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer
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