Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour

Detalhes bibliográficos
Autor(a) principal: Jara-Palacios, María José
Data de Publicação: 2020
Outros Autores: Gonçalves, Sandra, Heredia, Francisco J., Hernanz, Dolores, Romano, Anabela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/14751
Resumo: Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin-Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman's method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.
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spelling Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviourGrape pomaceUHPLCDPPHORACButyrylcholinesteraseElectrochemistryAcetylcholinesteraseExtraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin-Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman's method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.FCT: DL57/2016/CP1361/CT0022.MDPISapientiaJara-Palacios, María JoséGonçalves, SandraHeredia, Francisco J.Hernanz, DoloresRomano, Anabela2020-09-29T12:49:33Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14751eng2076-392110.3390/antiox9080675info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:27:05Zoai:sapientia.ualg.pt:10400.1/14751Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:43.875905Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
title Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
spellingShingle Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
Jara-Palacios, María José
Grape pomace
UHPLC
DPPH
ORAC
Butyrylcholinesterase
Electrochemistry
Acetylcholinesterase
title_short Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
title_full Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
title_fullStr Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
title_full_unstemmed Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
title_sort Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour
author Jara-Palacios, María José
author_facet Jara-Palacios, María José
Gonçalves, Sandra
Heredia, Francisco J.
Hernanz, Dolores
Romano, Anabela
author_role author
author2 Gonçalves, Sandra
Heredia, Francisco J.
Hernanz, Dolores
Romano, Anabela
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Jara-Palacios, María José
Gonçalves, Sandra
Heredia, Francisco J.
Hernanz, Dolores
Romano, Anabela
dc.subject.por.fl_str_mv Grape pomace
UHPLC
DPPH
ORAC
Butyrylcholinesterase
Electrochemistry
Acetylcholinesterase
topic Grape pomace
UHPLC
DPPH
ORAC
Butyrylcholinesterase
Electrochemistry
Acetylcholinesterase
description Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin-Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman's method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-29T12:49:33Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/14751
url http://hdl.handle.net/10400.1/14751
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-3921
10.3390/antiox9080675
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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