Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6852 |
Resumo: | Several different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned. |
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Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestineMicroparticlesEmulsion/solvent evaporation techniqueEnteric formsProtein stabilityProteolytic degradationInflammatory conditions of the intestinesSeveral different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaAmorim, Maria João Lopes Gonçalves de BritoFerreira, João Paulo Medeiros2011-10-22T17:02:20Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6852engAMORIM, Maria João Lopes Gonçalves de Brito, MEDEIROS, João Paulo Medeiros Ferreira - Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine. European Journal of Pharmaceutics and Biopharmaceutics. ISSN: 0939-6411. ISSN 0939-6411. Vol. 52, n.º 1 (2001), p. 39-44info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:37Zoai:repositorio.ucp.pt:10400.14/6852Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:51.645507Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
title |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
spellingShingle |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine Amorim, Maria João Lopes Gonçalves de Brito Microparticles Emulsion/solvent evaporation technique Enteric forms Protein stability Proteolytic degradation Inflammatory conditions of the intestines |
title_short |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
title_full |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
title_fullStr |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
title_full_unstemmed |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
title_sort |
Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine |
author |
Amorim, Maria João Lopes Gonçalves de Brito |
author_facet |
Amorim, Maria João Lopes Gonçalves de Brito Ferreira, João Paulo Medeiros |
author_role |
author |
author2 |
Ferreira, João Paulo Medeiros |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Amorim, Maria João Lopes Gonçalves de Brito Ferreira, João Paulo Medeiros |
dc.subject.por.fl_str_mv |
Microparticles Emulsion/solvent evaporation technique Enteric forms Protein stability Proteolytic degradation Inflammatory conditions of the intestines |
topic |
Microparticles Emulsion/solvent evaporation technique Enteric forms Protein stability Proteolytic degradation Inflammatory conditions of the intestines |
description |
Several different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 2001-01-01T00:00:00Z 2011-10-22T17:02:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6852 |
url |
http://hdl.handle.net/10400.14/6852 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
AMORIM, Maria João Lopes Gonçalves de Brito, MEDEIROS, João Paulo Medeiros Ferreira - Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine. European Journal of Pharmaceutics and Biopharmaceutics. ISSN: 0939-6411. ISSN 0939-6411. Vol. 52, n.º 1 (2001), p. 39-44 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131724432539648 |