Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine

Detalhes bibliográficos
Autor(a) principal: Amorim, Maria João Lopes Gonçalves de Brito
Data de Publicação: 2001
Outros Autores: Ferreira, João Paulo Medeiros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/6852
Resumo: Several different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned.
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spelling Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestineMicroparticlesEmulsion/solvent evaporation techniqueEnteric formsProtein stabilityProteolytic degradationInflammatory conditions of the intestinesSeveral different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaAmorim, Maria João Lopes Gonçalves de BritoFerreira, João Paulo Medeiros2011-10-22T17:02:20Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6852engAMORIM, Maria João Lopes Gonçalves de Brito, MEDEIROS, João Paulo Medeiros Ferreira - Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine. European Journal of Pharmaceutics and Biopharmaceutics. ISSN: 0939-6411. ISSN 0939-6411. Vol. 52, n.º 1 (2001), p. 39-44info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:37Zoai:repositorio.ucp.pt:10400.14/6852Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:51.645507Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
title Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
spellingShingle Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
Amorim, Maria João Lopes Gonçalves de Brito
Microparticles
Emulsion/solvent evaporation technique
Enteric forms
Protein stability
Proteolytic degradation
Inflammatory conditions of the intestines
title_short Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
title_full Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
title_fullStr Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
title_full_unstemmed Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
title_sort Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine
author Amorim, Maria João Lopes Gonçalves de Brito
author_facet Amorim, Maria João Lopes Gonçalves de Brito
Ferreira, João Paulo Medeiros
author_role author
author2 Ferreira, João Paulo Medeiros
author2_role author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Amorim, Maria João Lopes Gonçalves de Brito
Ferreira, João Paulo Medeiros
dc.subject.por.fl_str_mv Microparticles
Emulsion/solvent evaporation technique
Enteric forms
Protein stability
Proteolytic degradation
Inflammatory conditions of the intestines
topic Microparticles
Emulsion/solvent evaporation technique
Enteric forms
Protein stability
Proteolytic degradation
Inflammatory conditions of the intestines
description Several different peptides and proteins, such as the pancreatic trypsin inhibitor, growth factors and trefoil peptides, are known to play important roles in maintaining the structure and function of the gastrointestinal wall. With the advent of recombinant biotechnology, it has become feasible to test some of these proteins as therapeutics in different inflammatory conditions of the intestines. However, the harsh pH and enzymatic conditions of the stomach can lead to their inactivation. This research was aimed at the development of particulate, gastric-resistant pharmaceutical forms, incorporating those bioactive molecules. Mixtures of proteins in powder form were coated with cellulose acetate phthalate, Eudragit® S100 or Eudragit® RS PO, using simple preparation techniques based on single emulsion/solvent evaporation. Using aprotinin as a model drug, it was found that these procedures were effective in microencapsulating protein in the solid form without affecting its biological activity. Furthermore, and in particular with the first two polymers above, particles showed adequate in vitro release patterns for the applications envisioned.
publishDate 2001
dc.date.none.fl_str_mv 2001
2001-01-01T00:00:00Z
2011-10-22T17:02:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/6852
url http://hdl.handle.net/10400.14/6852
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv AMORIM, Maria João Lopes Gonçalves de Brito, MEDEIROS, João Paulo Medeiros Ferreira - Microparticles for delivering therapeutic peptides and proteins to the lumen of the small intestine. European Journal of Pharmaceutics and Biopharmaceutics. ISSN: 0939-6411. ISSN 0939-6411. Vol. 52, n.º 1 (2001), p. 39-44
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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