Expression of Lupinus albus PR-10 proteins during root and leaf development
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.15/452 |
Resumo: | Based on the NH2-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently used to screen the cDNA library. A cDNA coding for a PR-10 protein of 158 amino acid residues was cloned and sequenced. Subsequent studies involving Northern and Western blot analysis have shown that the PR-10 protein isoforms are differentially expressed during the development of the healthy lupin plant. High mRNA and protein contents were detected in roots and hypocotyls of both 7- and 20-d-old plants. In young leaves, the mRNA and protein contents were low and increasead in mature leaves. Tissue printing experiments with root sections suggest that the proteins are extracellular and are mainly associated with the vascular tissues in mature roots. |
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Expression of Lupinus albus PR-10 proteins during root and leaf developmentcDNA cloningDifferential protein expressionIntercellular spacesVascular tissuesWhite lupinLupinus albusBased on the NH2-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently used to screen the cDNA library. A cDNA coding for a PR-10 protein of 158 amino acid residues was cloned and sequenced. Subsequent studies involving Northern and Western blot analysis have shown that the PR-10 protein isoforms are differentially expressed during the development of the healthy lupin plant. High mRNA and protein contents were detected in roots and hypocotyls of both 7- and 20-d-old plants. In young leaves, the mRNA and protein contents were low and increasead in mature leaves. Tissue printing experiments with root sections suggest that the proteins are extracellular and are mainly associated with the vascular tissues in mature roots.SpringerRepositório Científico do Instituto Politécnico de SantarémPinto, PaulaRibeiro, A.Regalado, A. P.Rodrigues-Pousada, C.Ricardo, C. P. P.2011-06-03T08:39:46Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.15/452engPINTO, M.P.1; RIBEIRO, A. ; REGALADO; A. P. ; RODRIGUES-POUSADA, C.; RICARDO C. P. (2005) - Expression of Lupinus albus PR-10 proteins during root and leaf development. Biologia Plantarum, 49 (2): 187-193. DOI: 10.1007/s10535-005-7193-20006-3134info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-21T07:29:17Zoai:repositorio.ipsantarem.pt:10400.15/452Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:53:02.057482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
title |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
spellingShingle |
Expression of Lupinus albus PR-10 proteins during root and leaf development Pinto, Paula cDNA cloning Differential protein expression Intercellular spaces Vascular tissues White lupin Lupinus albus |
title_short |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
title_full |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
title_fullStr |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
title_full_unstemmed |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
title_sort |
Expression of Lupinus albus PR-10 proteins during root and leaf development |
author |
Pinto, Paula |
author_facet |
Pinto, Paula Ribeiro, A. Regalado, A. P. Rodrigues-Pousada, C. Ricardo, C. P. P. |
author_role |
author |
author2 |
Ribeiro, A. Regalado, A. P. Rodrigues-Pousada, C. Ricardo, C. P. P. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico de Santarém |
dc.contributor.author.fl_str_mv |
Pinto, Paula Ribeiro, A. Regalado, A. P. Rodrigues-Pousada, C. Ricardo, C. P. P. |
dc.subject.por.fl_str_mv |
cDNA cloning Differential protein expression Intercellular spaces Vascular tissues White lupin Lupinus albus |
topic |
cDNA cloning Differential protein expression Intercellular spaces Vascular tissues White lupin Lupinus albus |
description |
Based on the NH2-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently used to screen the cDNA library. A cDNA coding for a PR-10 protein of 158 amino acid residues was cloned and sequenced. Subsequent studies involving Northern and Western blot analysis have shown that the PR-10 protein isoforms are differentially expressed during the development of the healthy lupin plant. High mRNA and protein contents were detected in roots and hypocotyls of both 7- and 20-d-old plants. In young leaves, the mRNA and protein contents were low and increasead in mature leaves. Tissue printing experiments with root sections suggest that the proteins are extracellular and are mainly associated with the vascular tissues in mature roots. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z 2011-06-03T08:39:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.15/452 |
url |
http://hdl.handle.net/10400.15/452 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PINTO, M.P.1; RIBEIRO, A. ; REGALADO; A. P. ; RODRIGUES-POUSADA, C.; RICARDO C. P. (2005) - Expression of Lupinus albus PR-10 proteins during root and leaf development. Biologia Plantarum, 49 (2): 187-193. DOI: 10.1007/s10535-005-7193-2 0006-3134 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137021604659200 |