Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

Detalhes bibliográficos
Autor(a) principal: Sá, Marta
Data de Publicação: 2019
Outros Autores: Monte, Joana, Brazinha, Carla, Galinha, Claudia F., Crespo, João G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/114820
Resumo: This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT / MCTES ( UID/QUI/50006/2019 ); by the European FP7 KBBE project “D-Factory” (contract no. 613870); by KAUST OSR award no. OSR-2016-CPF-2907-05; grant of FCT / MCTES : SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel).
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spelling Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salinaCarotenogenesisDunaliella salinaFluorescence EEMsMembrane harvestingMonitoringAgronomy and Crop ScienceThis work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT / MCTES ( UID/QUI/50006/2019 ); by the European FP7 KBBE project “D-Factory” (contract no. 613870); by KAUST OSR award no. OSR-2016-CPF-2907-05; grant of FCT / MCTES : SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel).Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.DQ - Departamento de QuímicaLAQV@REQUIMTERUNSá, MartaMonte, JoanaBrazinha, CarlaGalinha, Claudia F.Crespo, João G.2022-03-31T00:31:39Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/114820eng2211-9264PURE: 15492723https://doi.org/10.1016/j.algal.2019.101720info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:57:38Zoai:run.unl.pt:10362/114820Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:38.999080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
title Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
spellingShingle Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
Sá, Marta
Carotenogenesis
Dunaliella salina
Fluorescence EEMs
Membrane harvesting
Monitoring
Agronomy and Crop Science
title_short Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
title_full Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
title_fullStr Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
title_full_unstemmed Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
title_sort Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina
author Sá, Marta
author_facet Sá, Marta
Monte, Joana
Brazinha, Carla
Galinha, Claudia F.
Crespo, João G.
author_role author
author2 Monte, Joana
Brazinha, Carla
Galinha, Claudia F.
Crespo, João G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Sá, Marta
Monte, Joana
Brazinha, Carla
Galinha, Claudia F.
Crespo, João G.
dc.subject.por.fl_str_mv Carotenogenesis
Dunaliella salina
Fluorescence EEMs
Membrane harvesting
Monitoring
Agronomy and Crop Science
topic Carotenogenesis
Dunaliella salina
Fluorescence EEMs
Membrane harvesting
Monitoring
Agronomy and Crop Science
description This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT / MCTES ( UID/QUI/50006/2019 ); by the European FP7 KBBE project “D-Factory” (contract no. 613870); by KAUST OSR award no. OSR-2016-CPF-2907-05; grant of FCT / MCTES : SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel).
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2019-12-01T00:00:00Z
2022-03-31T00:31:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/114820
url http://hdl.handle.net/10362/114820
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2211-9264
PURE: 15492723
https://doi.org/10.1016/j.algal.2019.101720
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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