Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/32981 https://doi.org/10.3389/fpls.2022.908308 |
Resumo: | Considered one of the most devastating plant–parasitic nematodes worldwide, Bursaphelenchus xylophilus (commonly known as pinewood nematode, PWN) is the causal agent of the pine wilt disease in the Eurasian coniferous forests. This migratory parasitic nematode is carried by an insect vector (Monochamus spp.) into the host tree (Pinus species), where it can feed on parenchymal cells and reproduce massively, resulting in the tree wilting. In declining trees, PWN populations are strongly dependent on fungal communities colonizing the host (predominantly ophiostomatoid fungi known to cause sapwood blue-staining, the blue-stain fungi), which not only influence their development and life cycle but also the number of individuals carried by the insect vector into a new host. Our main aim is to understand if PWN-associated mycobiota plays a key role in the development of PWD, in interaction with the PWN and the insect vector, and to what extent it can be targeted to disrupt the disease cycle. For this purpose, we characterized the fungal communities of Pinus pinaster trees infected and non-infected with PWN in three collection sites in Continental Portugal with different PWD temporal incidences. Our results showed that non-infected P. pinaster mycoflora is more diverse (in terms of abundance and fungal richness) than PWN-infected pine trees in the most recent PWD foci, as opposed to the fungal communities of long-term PWD history sites. Then, due to their ecological importance for PWN survival, representatives of the main ophiostomatoid fungi isolated (Ophiostoma, Leptographium, and Graphilbum) were characterized for their adaptative response to temperature, competition in-between taxa, and as food source for PWN. Under the conditions studied, Leptographium isolates showed promising results for PWN control. They could outcompete the other species, especially O. ips, and significantly reduce the development of PWN populations when compared to Botrytis cinerea (routinely used for PWN lab culturing), suggesting this to be a natural antagonist not only for the other blue-stain species but also for the PWN. |
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Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilusbiocontrolblue-stain fungidiversityecological interactionsmycoflorapinewood nematodeConsidered one of the most devastating plant–parasitic nematodes worldwide, Bursaphelenchus xylophilus (commonly known as pinewood nematode, PWN) is the causal agent of the pine wilt disease in the Eurasian coniferous forests. This migratory parasitic nematode is carried by an insect vector (Monochamus spp.) into the host tree (Pinus species), where it can feed on parenchymal cells and reproduce massively, resulting in the tree wilting. In declining trees, PWN populations are strongly dependent on fungal communities colonizing the host (predominantly ophiostomatoid fungi known to cause sapwood blue-staining, the blue-stain fungi), which not only influence their development and life cycle but also the number of individuals carried by the insect vector into a new host. Our main aim is to understand if PWN-associated mycobiota plays a key role in the development of PWD, in interaction with the PWN and the insect vector, and to what extent it can be targeted to disrupt the disease cycle. For this purpose, we characterized the fungal communities of Pinus pinaster trees infected and non-infected with PWN in three collection sites in Continental Portugal with different PWD temporal incidences. Our results showed that non-infected P. pinaster mycoflora is more diverse (in terms of abundance and fungal richness) than PWN-infected pine trees in the most recent PWD foci, as opposed to the fungal communities of long-term PWD history sites. Then, due to their ecological importance for PWN survival, representatives of the main ophiostomatoid fungi isolated (Ophiostoma, Leptographium, and Graphilbum) were characterized for their adaptative response to temperature, competition in-between taxa, and as food source for PWN. Under the conditions studied, Leptographium isolates showed promising results for PWN control. They could outcompete the other species, especially O. ips, and significantly reduce the development of PWN populations when compared to Botrytis cinerea (routinely used for PWN lab culturing), suggesting this to be a natural antagonist not only for the other blue-stain species but also for the PWN.Frontiers in Plant Science2022-12-29T16:05:06Z2022-12-292022-06-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/32981http://hdl.handle.net/10174/32981https://doi.org/10.3389/fpls.2022.908308engVicente CSL, Soares M, Faria JMS, Espada M, Mota M, Nóbrega F, Ramos AP, Inácio ML. Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus. Front Plant Sci. 2022 Jun 14;13:908308. doi: 10.3389/fpls.2022.908308.https://www.frontiersin.org/articles/10.3389/fpls.2022.908308/fullcvicente@uevora.ptndndmespada@uevora.ptndndndnd371Vicente, C. S. L.Soares, MiguelFaria, J. M. S.Margarida, EspadaManuel, MotaFilomena, NóbregaAna Paula, RamosMaria Lurdes, Inácioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:34:12Zoai:dspace.uevora.pt:10174/32981Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:21:52.550862Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
title |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
spellingShingle |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus Vicente, C. S. L. biocontrol blue-stain fungi diversity ecological interactions mycoflora pinewood nematode |
title_short |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
title_full |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
title_fullStr |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
title_full_unstemmed |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
title_sort |
Fungal communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales with Bursaphelenchus xylophilus |
author |
Vicente, C. S. L. |
author_facet |
Vicente, C. S. L. Soares, Miguel Faria, J. M. S. Margarida, Espada Manuel, Mota Filomena, Nóbrega Ana Paula, Ramos Maria Lurdes, Inácio |
author_role |
author |
author2 |
Soares, Miguel Faria, J. M. S. Margarida, Espada Manuel, Mota Filomena, Nóbrega Ana Paula, Ramos Maria Lurdes, Inácio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vicente, C. S. L. Soares, Miguel Faria, J. M. S. Margarida, Espada Manuel, Mota Filomena, Nóbrega Ana Paula, Ramos Maria Lurdes, Inácio |
dc.subject.por.fl_str_mv |
biocontrol blue-stain fungi diversity ecological interactions mycoflora pinewood nematode |
topic |
biocontrol blue-stain fungi diversity ecological interactions mycoflora pinewood nematode |
description |
Considered one of the most devastating plant–parasitic nematodes worldwide, Bursaphelenchus xylophilus (commonly known as pinewood nematode, PWN) is the causal agent of the pine wilt disease in the Eurasian coniferous forests. This migratory parasitic nematode is carried by an insect vector (Monochamus spp.) into the host tree (Pinus species), where it can feed on parenchymal cells and reproduce massively, resulting in the tree wilting. In declining trees, PWN populations are strongly dependent on fungal communities colonizing the host (predominantly ophiostomatoid fungi known to cause sapwood blue-staining, the blue-stain fungi), which not only influence their development and life cycle but also the number of individuals carried by the insect vector into a new host. Our main aim is to understand if PWN-associated mycobiota plays a key role in the development of PWD, in interaction with the PWN and the insect vector, and to what extent it can be targeted to disrupt the disease cycle. For this purpose, we characterized the fungal communities of Pinus pinaster trees infected and non-infected with PWN in three collection sites in Continental Portugal with different PWD temporal incidences. Our results showed that non-infected P. pinaster mycoflora is more diverse (in terms of abundance and fungal richness) than PWN-infected pine trees in the most recent PWD foci, as opposed to the fungal communities of long-term PWD history sites. Then, due to their ecological importance for PWN survival, representatives of the main ophiostomatoid fungi isolated (Ophiostoma, Leptographium, and Graphilbum) were characterized for their adaptative response to temperature, competition in-between taxa, and as food source for PWN. Under the conditions studied, Leptographium isolates showed promising results for PWN control. They could outcompete the other species, especially O. ips, and significantly reduce the development of PWN populations when compared to Botrytis cinerea (routinely used for PWN lab culturing), suggesting this to be a natural antagonist not only for the other blue-stain species but also for the PWN. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-29T16:05:06Z 2022-12-29 2022-06-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/32981 http://hdl.handle.net/10174/32981 https://doi.org/10.3389/fpls.2022.908308 |
url |
http://hdl.handle.net/10174/32981 https://doi.org/10.3389/fpls.2022.908308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vicente CSL, Soares M, Faria JMS, Espada M, Mota M, Nóbrega F, Ramos AP, Inácio ML. Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus. Front Plant Sci. 2022 Jun 14;13:908308. doi: 10.3389/fpls.2022.908308. https://www.frontiersin.org/articles/10.3389/fpls.2022.908308/full cvicente@uevora.pt nd nd mespada@uevora.pt nd nd nd nd 371 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers in Plant Science |
publisher.none.fl_str_mv |
Frontiers in Plant Science |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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