Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira

Detalhes bibliográficos
Autor(a) principal: Freitas, I. R. A. de
Data de Publicação: 2005
Outros Autores: Ganança, F., Santos, T. M. dos, Carvalho, M. A. A. Pinheiro de, Motto, M., Vieira, M. Clemente
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/3185
Resumo: Zein polypeptides are a group of proteins that accumulate in maize endosperm during seed development, representing more than 60% of the total endosperm proteins in the mature seeds. To evaluate genotype variability of Madeiran maize germplasm, a biochemical study was conducted based on the prolamins of maize, zeins, extracted from endosperm meal of 43 populations of Zea mays L. maintained in the Germplasm bank of Madeira University along with the inbreed W64A which was used as a polymorphic zein polypeptide standard profile. The zein polymorphism of these 44 maize populations were compared using two different electrophoresis techniques, SDS-PAGE in 15% discontinuous polyacrylamide gel and Acid-PAGE in 10% continuous polyacrylamide gel. SDS-PAGE allowed up to 16 polypeptides to be identified with apparent molecular mass ranging from 28-kDa to 10-kDa. Acid-PAGE allowed up to 20 zein fractions to be identified. The data was submitted to principal component analysis (PCA) and canonical discriminate and similarity analysis. The SDS-PAGE zein polymorphism allowed us to detect 6 groups, assembling all maize populations and explaining 55.32% of all variability. The similarity analysis of zein patterns obtained by Acid-PAGE showed that among regional maize germplasm, 22.5 % of all population seems to be related and have a common ancestor. The ISOP71 seems to be the population more closed to the common ancestor and appears related with the remaining maize populations, excluding the ISOP125.The obtained results and the importance of zein polymorphism in the evaluation of maize germplasm from Madeiran Archipelago are discussed
id RCAP_48f81d6eb50bbbce41af51eafc2e1fdd
oai_identifier_str oai:digituma.uma.pt:10400.13/3185
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evaluation of maize germplasm based on zein polymorphism from the archipelago of MadeiraSeed storage proteinsZeinSDS-PAGEAcid-PAGEMaize germplasmArchipelago of Madeira (Portugal).Faculdade de Ciências da VidaZein polypeptides are a group of proteins that accumulate in maize endosperm during seed development, representing more than 60% of the total endosperm proteins in the mature seeds. To evaluate genotype variability of Madeiran maize germplasm, a biochemical study was conducted based on the prolamins of maize, zeins, extracted from endosperm meal of 43 populations of Zea mays L. maintained in the Germplasm bank of Madeira University along with the inbreed W64A which was used as a polymorphic zein polypeptide standard profile. The zein polymorphism of these 44 maize populations were compared using two different electrophoresis techniques, SDS-PAGE in 15% discontinuous polyacrylamide gel and Acid-PAGE in 10% continuous polyacrylamide gel. SDS-PAGE allowed up to 16 polypeptides to be identified with apparent molecular mass ranging from 28-kDa to 10-kDa. Acid-PAGE allowed up to 20 zein fractions to be identified. The data was submitted to principal component analysis (PCA) and canonical discriminate and similarity analysis. The SDS-PAGE zein polymorphism allowed us to detect 6 groups, assembling all maize populations and explaining 55.32% of all variability. The similarity analysis of zein patterns obtained by Acid-PAGE showed that among regional maize germplasm, 22.5 % of all population seems to be related and have a common ancestor. The ISOP71 seems to be the population more closed to the common ancestor and appears related with the remaining maize populations, excluding the ISOP125.The obtained results and the importance of zein polymorphism in the evaluation of maize germplasm from Madeiran Archipelago are discussedUnità di Ricerca per la Valorizzazione qualitativa dei cerealiDigitUMaFreitas, I. R. A. deGanança, F.Santos, T. M. dosCarvalho, M. A. A. Pinheiro deMotto, M.Vieira, M. Clemente2021-03-19T10:38:30Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3185engFreitas, I. R. A., Ganança, F., Santos, T. M., Carvalho, M. A. A. P., Motto, M., & Vieira, M. C. (2005). Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira. Maydica, 50(2), 105-112.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-26T03:39:08Zoai:digituma.uma.pt:10400.13/3185Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:08.191413Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
title Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
spellingShingle Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
Freitas, I. R. A. de
Seed storage proteins
Zein
SDS-PAGE
Acid-PAGE
Maize germplasm
Archipelago of Madeira (Portugal)
.
Faculdade de Ciências da Vida
title_short Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
title_full Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
title_fullStr Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
title_full_unstemmed Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
title_sort Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira
author Freitas, I. R. A. de
author_facet Freitas, I. R. A. de
Ganança, F.
Santos, T. M. dos
Carvalho, M. A. A. Pinheiro de
Motto, M.
Vieira, M. Clemente
author_role author
author2 Ganança, F.
Santos, T. M. dos
Carvalho, M. A. A. Pinheiro de
Motto, M.
Vieira, M. Clemente
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Freitas, I. R. A. de
Ganança, F.
Santos, T. M. dos
Carvalho, M. A. A. Pinheiro de
Motto, M.
Vieira, M. Clemente
dc.subject.por.fl_str_mv Seed storage proteins
Zein
SDS-PAGE
Acid-PAGE
Maize germplasm
Archipelago of Madeira (Portugal)
.
Faculdade de Ciências da Vida
topic Seed storage proteins
Zein
SDS-PAGE
Acid-PAGE
Maize germplasm
Archipelago of Madeira (Portugal)
.
Faculdade de Ciências da Vida
description Zein polypeptides are a group of proteins that accumulate in maize endosperm during seed development, representing more than 60% of the total endosperm proteins in the mature seeds. To evaluate genotype variability of Madeiran maize germplasm, a biochemical study was conducted based on the prolamins of maize, zeins, extracted from endosperm meal of 43 populations of Zea mays L. maintained in the Germplasm bank of Madeira University along with the inbreed W64A which was used as a polymorphic zein polypeptide standard profile. The zein polymorphism of these 44 maize populations were compared using two different electrophoresis techniques, SDS-PAGE in 15% discontinuous polyacrylamide gel and Acid-PAGE in 10% continuous polyacrylamide gel. SDS-PAGE allowed up to 16 polypeptides to be identified with apparent molecular mass ranging from 28-kDa to 10-kDa. Acid-PAGE allowed up to 20 zein fractions to be identified. The data was submitted to principal component analysis (PCA) and canonical discriminate and similarity analysis. The SDS-PAGE zein polymorphism allowed us to detect 6 groups, assembling all maize populations and explaining 55.32% of all variability. The similarity analysis of zein patterns obtained by Acid-PAGE showed that among regional maize germplasm, 22.5 % of all population seems to be related and have a common ancestor. The ISOP71 seems to be the population more closed to the common ancestor and appears related with the remaining maize populations, excluding the ISOP125.The obtained results and the importance of zein polymorphism in the evaluation of maize germplasm from Madeiran Archipelago are discussed
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
2021-03-19T10:38:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/3185
url http://hdl.handle.net/10400.13/3185
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Freitas, I. R. A., Ganança, F., Santos, T. M., Carvalho, M. A. A. P., Motto, M., & Vieira, M. C. (2005). Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira. Maydica, 50(2), 105-112.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Unità di Ricerca per la Valorizzazione qualitativa dei cereali
publisher.none.fl_str_mv Unità di Ricerca per la Valorizzazione qualitativa dei cereali
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799129932392038400