Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/457 |
Resumo: | Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies. |
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Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root NodulesCellsGene Expression ProfilingMedicago truncatulaRoot Nodules, PlantLegumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies.Dutch Organization for Scientific Research grant: (184319448).PLOSARCALimpens, ErikMoling, SjefHooiveld, GuidoPereira, Patrícia A.Bisseling, TonBecker, Jörg D.Küster, Helge2015-11-02T15:43:32Z2013-05-292013-05-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/457engLimpens E, Moling S, Hooiveld G, Pereira PA, Bisseling T, Becker JD, et al. (2013) Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. PLoS ONE 8(5): e64377. doi:10.1371/journal.pone.006437710.1371/journal.pone.0064377info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:51Zoai:arca.igc.gulbenkian.pt:10400.7/457Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:44.409579Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
title |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
spellingShingle |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules Limpens, Erik Cells Gene Expression Profiling Medicago truncatula Root Nodules, Plant |
title_short |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
title_full |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
title_fullStr |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
title_full_unstemmed |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
title_sort |
Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules |
author |
Limpens, Erik |
author_facet |
Limpens, Erik Moling, Sjef Hooiveld, Guido Pereira, Patrícia A. Bisseling, Ton Becker, Jörg D. Küster, Helge |
author_role |
author |
author2 |
Moling, Sjef Hooiveld, Guido Pereira, Patrícia A. Bisseling, Ton Becker, Jörg D. Küster, Helge |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Limpens, Erik Moling, Sjef Hooiveld, Guido Pereira, Patrícia A. Bisseling, Ton Becker, Jörg D. Küster, Helge |
dc.subject.por.fl_str_mv |
Cells Gene Expression Profiling Medicago truncatula Root Nodules, Plant |
topic |
Cells Gene Expression Profiling Medicago truncatula Root Nodules, Plant |
description |
Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-29 2013-05-29T00:00:00Z 2015-11-02T15:43:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/457 |
url |
http://hdl.handle.net/10400.7/457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Limpens E, Moling S, Hooiveld G, Pereira PA, Bisseling T, Becker JD, et al. (2013) Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. PLoS ONE 8(5): e64377. doi:10.1371/journal.pone.0064377 10.1371/journal.pone.0064377 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130572937756672 |