A Protocol for FRET-Based Live-Cell Imaging in Microglia

Detalhes bibliográficos
Autor(a) principal: Socodato, R
Data de Publicação: 2020
Outros Autores: Melo, PN, Ferraz-Nogueira, JP, Portugal, CC, Relvas, JB
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/145266
Resumo: This protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).
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spelling A Protocol for FRET-Based Live-Cell Imaging in MicrogliaThis protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).Cell Press20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/145266eng2666-166710.1016/j.xpro.2020.100147Socodato, RMelo, PNFerraz-Nogueira, JPPortugal, CCRelvas, JBinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:46:03Zoai:repositorio-aberto.up.pt:10216/145266Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:08:01.727055Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A Protocol for FRET-Based Live-Cell Imaging in Microglia
title A Protocol for FRET-Based Live-Cell Imaging in Microglia
spellingShingle A Protocol for FRET-Based Live-Cell Imaging in Microglia
Socodato, R
title_short A Protocol for FRET-Based Live-Cell Imaging in Microglia
title_full A Protocol for FRET-Based Live-Cell Imaging in Microglia
title_fullStr A Protocol for FRET-Based Live-Cell Imaging in Microglia
title_full_unstemmed A Protocol for FRET-Based Live-Cell Imaging in Microglia
title_sort A Protocol for FRET-Based Live-Cell Imaging in Microglia
author Socodato, R
author_facet Socodato, R
Melo, PN
Ferraz-Nogueira, JP
Portugal, CC
Relvas, JB
author_role author
author2 Melo, PN
Ferraz-Nogueira, JP
Portugal, CC
Relvas, JB
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Socodato, R
Melo, PN
Ferraz-Nogueira, JP
Portugal, CC
Relvas, JB
description This protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/145266
url https://hdl.handle.net/10216/145266
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2666-1667
10.1016/j.xpro.2020.100147
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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