MicroRNA expression profile of Danio rerio brain exposed to ethanol
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/8982 |
Resumo: | miRNAs are short (~22 nt) non-coding RNA molecules that control gene expression at post-transcription level by degrading or inhibiting particular target genes. miRNAs are abundant in vertebrate genomes where they play an important role on the control of cell proliferation and differentiation, apoptosis, neurogenesis and synaptic plasticity. Although legally commercialize, alcohol is toxic for living organisms and its consumption can induce dependence. The regular alcohol intake increases the risk of hepatic disease and certain cancers, having a deleterious effect on the immune, endocrine and nervous system. The brain is not only an important target for alcohol metabolites, but also a rich source of miRNAs. Using miRNA microarrays approach, we aimed to understand how chronic (0.25% EtOH) and acute (0.25%; 0.5%, 1 e 1.5% EtOH) ethanol exposure affects miRNA expression. The results demonstrate that chronic ethanol intake deregulated the expression of 32 miRNAs. From those, miR-9, miR-23a, miR-30e, miR-133a, miR-181 were over-expressed and miR-16a, miR-145 and miR-181b were inhibited. The putative target genes for those miRNAs are implicated in cell cycle, differentiation, apoptosis and cell adhesion. The acute ethanol exposure also deregulated miRNA expression profile and each ethanol concentration shows a distinct miRNA expression profile. For example, the pro-apoptotic miRNA miR- 23a is up-regulated in chronic ethanol exposure but is down-regulated in the higher ethanol concentrations (1 e 1.5% EtOH) of acute test. These results suggest that alcohol consumption, even consumed in a short-period or concentration, affects the expression of miRNAs. Possibly, these alterations have implications on cellular cycle and apoptosis and therefore they could contribute to a higher risk of developing tumours. |
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MicroRNA expression profile of Danio rerio brain exposed to ethanolBiologia molecularAlcoolismoRegulação genéticamiRNAs are short (~22 nt) non-coding RNA molecules that control gene expression at post-transcription level by degrading or inhibiting particular target genes. miRNAs are abundant in vertebrate genomes where they play an important role on the control of cell proliferation and differentiation, apoptosis, neurogenesis and synaptic plasticity. Although legally commercialize, alcohol is toxic for living organisms and its consumption can induce dependence. The regular alcohol intake increases the risk of hepatic disease and certain cancers, having a deleterious effect on the immune, endocrine and nervous system. The brain is not only an important target for alcohol metabolites, but also a rich source of miRNAs. Using miRNA microarrays approach, we aimed to understand how chronic (0.25% EtOH) and acute (0.25%; 0.5%, 1 e 1.5% EtOH) ethanol exposure affects miRNA expression. The results demonstrate that chronic ethanol intake deregulated the expression of 32 miRNAs. From those, miR-9, miR-23a, miR-30e, miR-133a, miR-181 were over-expressed and miR-16a, miR-145 and miR-181b were inhibited. The putative target genes for those miRNAs are implicated in cell cycle, differentiation, apoptosis and cell adhesion. The acute ethanol exposure also deregulated miRNA expression profile and each ethanol concentration shows a distinct miRNA expression profile. For example, the pro-apoptotic miRNA miR- 23a is up-regulated in chronic ethanol exposure but is down-regulated in the higher ethanol concentrations (1 e 1.5% EtOH) of acute test. These results suggest that alcohol consumption, even consumed in a short-period or concentration, affects the expression of miRNAs. Possibly, these alterations have implications on cellular cycle and apoptosis and therefore they could contribute to a higher risk of developing tumours.Os miRNAs são pequenas moléculas (~22 nt) de RNA não-codificante que regulam a expressão genética ao nível pós-transcripcional através da degradação ou inibição dos genes alvo. Os miRNAs são abundantes nos genomas eucariótas, onde desempenham funções importantes no controlo da proliferação e diferenciação celular, apoptose, neurogénese e plasticidade sináptica. Apesar de ser uma substância legal, o álcool é tóxico para os seres vivos e o seu consumo regular pode induzir dependência. O consumo excessivo de álcool aumenta o risco de doença hepática e de determinados cancros e tem efeitos deletérios ao nível do sistema imunitário, endócrino e nervoso. O cérebro, para além de ser um importante alvo dos metabolitos do álcool, é um dos órgãos com maior expressão de miRNAs. Através da análise de microarrays de miRNAs pretendeu-se entender de que modo a exposição crónica ao etanol (0.25% EtOH) e a exposição aguda a um pulso de etanol (0.25%; 0.5%, 1 e 1.5% EtOH) afectam a expressão dos miRNAs. A expressão de 32 miRNAs foi significativamente alterada pela exposição crónica ao etanol, destacando-se o aumento da expressão do miR-9, miR-23a, miR-30e, miR- 133a, miR-181 e a diminuição na expressão do miR-16a, miR-145 e miR-181b. Genes envolvidos no ciclo celular, diferenciação, apoptose e adesão celular parecem ser os alvos preferenciais destes miRNAs. A exposição aguda também alterou a expressão de vários miRNAs, que variam consoante a concentração de etanol utilizada. Por exemplo, a expressão do miRNA próapoptótico miR-23a aumentou durante a exposição crónica ao etanol e diminuiu com o aumento da concentração do tóxico (1 e 1.5% EtOH) durante o teste agudo. Estes resultados sugerem que o consumo de álcool, mesmo num curto espaço de tempo ou concentração, afecta a expressão dos miRNAs. Possivelmente essas alterações têm implicações no ciclo celular e apotose, podendo contribuir deste modo para um risco acrescido de desenvolver tumores.Universidade de Aveiro2012-09-10T07:50:40Z2010-01-01T00:00:00Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/8982engFerreira, Violeta Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:15:21Zoai:ria.ua.pt:10773/8982Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:45:57.712292Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
title |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
spellingShingle |
MicroRNA expression profile of Danio rerio brain exposed to ethanol Ferreira, Violeta Silva Biologia molecular Alcoolismo Regulação genética |
title_short |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
title_full |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
title_fullStr |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
title_full_unstemmed |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
title_sort |
MicroRNA expression profile of Danio rerio brain exposed to ethanol |
author |
Ferreira, Violeta Silva |
author_facet |
Ferreira, Violeta Silva |
author_role |
author |
dc.contributor.author.fl_str_mv |
Ferreira, Violeta Silva |
dc.subject.por.fl_str_mv |
Biologia molecular Alcoolismo Regulação genética |
topic |
Biologia molecular Alcoolismo Regulação genética |
description |
miRNAs are short (~22 nt) non-coding RNA molecules that control gene expression at post-transcription level by degrading or inhibiting particular target genes. miRNAs are abundant in vertebrate genomes where they play an important role on the control of cell proliferation and differentiation, apoptosis, neurogenesis and synaptic plasticity. Although legally commercialize, alcohol is toxic for living organisms and its consumption can induce dependence. The regular alcohol intake increases the risk of hepatic disease and certain cancers, having a deleterious effect on the immune, endocrine and nervous system. The brain is not only an important target for alcohol metabolites, but also a rich source of miRNAs. Using miRNA microarrays approach, we aimed to understand how chronic (0.25% EtOH) and acute (0.25%; 0.5%, 1 e 1.5% EtOH) ethanol exposure affects miRNA expression. The results demonstrate that chronic ethanol intake deregulated the expression of 32 miRNAs. From those, miR-9, miR-23a, miR-30e, miR-133a, miR-181 were over-expressed and miR-16a, miR-145 and miR-181b were inhibited. The putative target genes for those miRNAs are implicated in cell cycle, differentiation, apoptosis and cell adhesion. The acute ethanol exposure also deregulated miRNA expression profile and each ethanol concentration shows a distinct miRNA expression profile. For example, the pro-apoptotic miRNA miR- 23a is up-regulated in chronic ethanol exposure but is down-regulated in the higher ethanol concentrations (1 e 1.5% EtOH) of acute test. These results suggest that alcohol consumption, even consumed in a short-period or concentration, affects the expression of miRNAs. Possibly, these alterations have implications on cellular cycle and apoptosis and therefore they could contribute to a higher risk of developing tumours. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01T00:00:00Z 2010 2012-09-10T07:50:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/8982 |
url |
http://hdl.handle.net/10773/8982 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Aveiro |
publisher.none.fl_str_mv |
Universidade de Aveiro |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137512089714688 |