Desenvolvimento de novas abordagens terapêuticas para a regeneração da pele

• Autor(a) principal: Figueira, Daniela Sofia Rodrigues
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.6/4728
• topic: Electrofiação; Engenharia de tecidos; Membrana em bicamada; Ácido salicílico; Domínio/Área Científica::Ciências Médicas::Ciências Biomédicas

Every year, millions of patients suffer burns, chronic or surgical-related wounds. Autografts are still the gold standard used for the treatment of these injuries, although they have several drawbacks like limited availability of donor sites, patient morbidity, and long periods of hospitalization. To surpass such drawbacks, several studies have been focused on the development of polymeric matrices that are able to reproduce the skin native structure and also improve its regeneration. Herein, a bilayer membrane was produced by electrospinning and its properties have been characterized through in vitro assays. The upper layer of the membrane was comprised of hyaluronic acid and polycaprolactone in order to provide mechanical support and also to act as a physical barrier against external threats. Chitosan and zein were used to produce the bottom layer. Furthermore, salicylic acid was also incorporated in this layer for conferring anti-inflammatory and antimicrobial properties to the membrane. The obtained results showed that the produced electrospun meshes display an ideal porosity, appropriate mechanical properties, controlled evaporative water loss and an initial burst release of SA. Moreover, the membranes did not exhibit any toxic effects for human fibroblast cells and promoted their adhesion, spread, and proliferation. In addition, no biofilm formation was noticed on their surface along the experiments. The obtained data reveal that this electrospun membrane possesses the required properties to be used in wound healing.