Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/21404 |
Resumo: | Metabolite profiling of E. coli W3110 and the isogenic ∆relA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism. |
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Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat culturesMetabolomicsMetabolite profilesStringent responseRelaxed phenotypeScience & TechnologyMetabolite profiling of E. coli W3110 and the isogenic ∆relA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism.The authors thank to Raphael Aggio for assisting in the automatic refinement and correction of the GC-MS data, Katie Smart for performing acetate analyses and Clark Ehlers for his support with the bioreactor set up. This work was supported by the Portuguese FCT (Fundacao para a Ciencia e Tecnologia) funded MIT-Portugal Program in Bioengineering (MIT-Pt/BS-BB/0082/2008) and by ERDF-European Regional Development Fund through the COMPETE Programme (operational programme for competitiveness) and by National Funds through the FCT (Portuguese Foundation for Science and Technology) within the project FCOMP-01-0124-FEDER-009707 (HeliSysBio. molecular Systems Biology in Helicobacter pylori). The work was also supported by a PhD grant from FCT (ref. SFRH/BD/22863/2005).MDPIUniversidade do MinhoCarneiro, S.Villas-Bôas, S. G.Ferreira, Eugénio C.Rocha, I.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/21404eng2218-198910.3390/metabo2040717info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:32:26Zoai:repositorium.sdum.uminho.pt:1822/21404Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:27:46.419590Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
title |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
spellingShingle |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures Carneiro, S. Metabolomics Metabolite profiles Stringent response Relaxed phenotype Science & Technology |
title_short |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
title_full |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
title_fullStr |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
title_full_unstemmed |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
title_sort |
Influence of the RelA activity on E. coli metabolism by metabolite profiling of glucose-limited chemostat cultures |
author |
Carneiro, S. |
author_facet |
Carneiro, S. Villas-Bôas, S. G. Ferreira, Eugénio C. Rocha, I. |
author_role |
author |
author2 |
Villas-Bôas, S. G. Ferreira, Eugénio C. Rocha, I. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Carneiro, S. Villas-Bôas, S. G. Ferreira, Eugénio C. Rocha, I. |
dc.subject.por.fl_str_mv |
Metabolomics Metabolite profiles Stringent response Relaxed phenotype Science & Technology |
topic |
Metabolomics Metabolite profiles Stringent response Relaxed phenotype Science & Technology |
description |
Metabolite profiling of E. coli W3110 and the isogenic ∆relA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/21404 |
url |
https://hdl.handle.net/1822/21404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2218-1989 10.3390/metabo2040717 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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