A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/2611 |
Resumo: | How can odor-guided behavior of numerous individual Drosophila be assessed automatically with high temporal resolution? For this purpose we introduce the automatic integrated tracking and odor-delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor pulses, well defined in concentration and timing. The flies' positions are visually tracked, which allows quantification of the odor-evoked walking behavior with high temporal resolution of up to 100 ms. As a demonstration of Flywalk we show that the flies' behavior is odorant-specific; attractive odors elicit directed upwind movements, while repellent odors evoke decreased activity, followed by downwind movements. These changes in behavior differ between sexes. Furthermore our findings show that flies can evaluate the sex of a conspecific and males can determine a female's mating status based on olfactory cues. Consequently, Flywalk allows automatic screening of individual flies for their olfactory preference and sensitivity. |
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A high-throughput behavioral paradigm for Drosophila olfaction - The FlywalkAnimal behaviourBehaviourBiological modelsSensory sistemsOdorantsDrosophila olfaction.Faculdade de Ciências Exatas e da EngenhariaHow can odor-guided behavior of numerous individual Drosophila be assessed automatically with high temporal resolution? For this purpose we introduce the automatic integrated tracking and odor-delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor pulses, well defined in concentration and timing. The flies' positions are visually tracked, which allows quantification of the odor-evoked walking behavior with high temporal resolution of up to 100 ms. As a demonstration of Flywalk we show that the flies' behavior is odorant-specific; attractive odors elicit directed upwind movements, while repellent odors evoke decreased activity, followed by downwind movements. These changes in behavior differ between sexes. Furthermore our findings show that flies can evaluate the sex of a conspecific and males can determine a female's mating status based on olfactory cues. Consequently, Flywalk allows automatic screening of individual flies for their olfactory preference and sensitivity.DigitUMaSteck, KathrinVeit, DanielGrandy, RonaldBermúdez i Badia, SergiMathews, ZenonVerschure, PaulHansson, Bill S.Knaden, Markus2019-12-03T14:12:30Z2012-01-01T00:00:00Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/2611engSteck, K., Veit, D., Grandy, R., Bermúdez i Badia, S., Mathews, Z., Verschure, P., ... & Knaden, M. (2012). A high-throughput behavioral paradigm for Drosophila olfaction-The Flywalk. Scientific reports, 2, 361.10.1038/srep00361info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:55:26Zoai:digituma.uma.pt:10400.13/2611Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:05:23.732449Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
title |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
spellingShingle |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk Steck, Kathrin Animal behaviour Behaviour Biological models Sensory sistems Odorants Drosophila olfaction . Faculdade de Ciências Exatas e da Engenharia |
title_short |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
title_full |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
title_fullStr |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
title_full_unstemmed |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
title_sort |
A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk |
author |
Steck, Kathrin |
author_facet |
Steck, Kathrin Veit, Daniel Grandy, Ronald Bermúdez i Badia, Sergi Mathews, Zenon Verschure, Paul Hansson, Bill S. Knaden, Markus |
author_role |
author |
author2 |
Veit, Daniel Grandy, Ronald Bermúdez i Badia, Sergi Mathews, Zenon Verschure, Paul Hansson, Bill S. Knaden, Markus |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Steck, Kathrin Veit, Daniel Grandy, Ronald Bermúdez i Badia, Sergi Mathews, Zenon Verschure, Paul Hansson, Bill S. Knaden, Markus |
dc.subject.por.fl_str_mv |
Animal behaviour Behaviour Biological models Sensory sistems Odorants Drosophila olfaction . Faculdade de Ciências Exatas e da Engenharia |
topic |
Animal behaviour Behaviour Biological models Sensory sistems Odorants Drosophila olfaction . Faculdade de Ciências Exatas e da Engenharia |
description |
How can odor-guided behavior of numerous individual Drosophila be assessed automatically with high temporal resolution? For this purpose we introduce the automatic integrated tracking and odor-delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor pulses, well defined in concentration and timing. The flies' positions are visually tracked, which allows quantification of the odor-evoked walking behavior with high temporal resolution of up to 100 ms. As a demonstration of Flywalk we show that the flies' behavior is odorant-specific; attractive odors elicit directed upwind movements, while repellent odors evoke decreased activity, followed by downwind movements. These changes in behavior differ between sexes. Furthermore our findings show that flies can evaluate the sex of a conspecific and males can determine a female's mating status based on olfactory cues. Consequently, Flywalk allows automatic screening of individual flies for their olfactory preference and sensitivity. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01T00:00:00Z 2012-01-01T00:00:00Z 2019-12-03T14:12:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/2611 |
url |
http://hdl.handle.net/10400.13/2611 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Steck, K., Veit, D., Grandy, R., Bermúdez i Badia, S., Mathews, Z., Verschure, P., ... & Knaden, M. (2012). A high-throughput behavioral paradigm for Drosophila olfaction-The Flywalk. Scientific reports, 2, 361. 10.1038/srep00361 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799129925192515584 |