Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/5424 |
Resumo: | Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, Crassostrea gigas, a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3-month laboratory-based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH. Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI3K/AKT/mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C. gigas which could significantly impact on coastal ecosystem biodiversity and function in the future. |
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Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigasCandidate genesCondition indexEnergetic trade-offsmTOR pathwayTranscriptional profilingUnderstanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, Crassostrea gigas, a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3-month laboratory-based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH. Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI3K/AKT/mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C. gigas which could significantly impact on coastal ecosystem biodiversity and function in the future.This work was supported by an EU Research Infrastructure Action under the EP7 “Capacities” Specific Programme, ASSEMBLE grant agreement no. 227799, CCMAR Ref 00415/2010. We thank Lloyd Peck (BAS) for advice on physiology and critical reading of the manuscript, Simon Morley (BAS) for statistical analyses, John Turner (BAS) for the Hadley Centre predictions, Elizabeth Harper Cambridge University for damage repair analysis, and Ana Grade (IPMA) who assisted with RNA extractions.WileySapientiaClark, M. S.Thorne, Michael A. S.Araújo Amaral, Ana MargaridaVieira, Florbela A.Batista, FredericoReis, JoãoPower, Deborah2014-10-23T10:18:02Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5424engMelody S. Clark, Michael A. S. Thorne, Ana Amaral, Florbela Vieira, Frederico M. Batista, João Reis & Deborah M. Power, "Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas" in Ecology and Evolution 2013; 3(10): 3283– 32972045-7758AUT: DPO00386;http://dx.doi.org/10.1002/ece3.719info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:39Zoai:sapientia.ualg.pt:10400.1/5424Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:29.442700Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
title |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
spellingShingle |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas Clark, M. S. Candidate genes Condition index Energetic trade-offs mTOR pathway Transcriptional profiling |
title_short |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
title_full |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
title_fullStr |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
title_sort |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas |
author |
Clark, M. S. |
author_facet |
Clark, M. S. Thorne, Michael A. S. Araújo Amaral, Ana Margarida Vieira, Florbela A. Batista, Frederico Reis, João Power, Deborah |
author_role |
author |
author2 |
Thorne, Michael A. S. Araújo Amaral, Ana Margarida Vieira, Florbela A. Batista, Frederico Reis, João Power, Deborah |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Clark, M. S. Thorne, Michael A. S. Araújo Amaral, Ana Margarida Vieira, Florbela A. Batista, Frederico Reis, João Power, Deborah |
dc.subject.por.fl_str_mv |
Candidate genes Condition index Energetic trade-offs mTOR pathway Transcriptional profiling |
topic |
Candidate genes Condition index Energetic trade-offs mTOR pathway Transcriptional profiling |
description |
Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, Crassostrea gigas, a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3-month laboratory-based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH. Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI3K/AKT/mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C. gigas which could significantly impact on coastal ecosystem biodiversity and function in the future. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2014-10-23T10:18:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/5424 |
url |
http://hdl.handle.net/10400.1/5424 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Melody S. Clark, Michael A. S. Thorne, Ana Amaral, Florbela Vieira, Frederico M. Batista, João Reis & Deborah M. Power, "Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas" in Ecology and Evolution 2013; 3(10): 3283– 3297 2045-7758 AUT: DPO00386; http://dx.doi.org/10.1002/ece3.719 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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