Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/8556 |
Resumo: | Aim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics. |
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Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studiesWild mushroomsAntimicrobial activityClinical isolatesSARDockingAim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics.Fundação para a Ciência e a Tecnologia (FCT, Portugal) and COMPETE/QREN/EU for financial support to this work (research project PTDC/AGR-ALI/110062/2009) and to CIMO (strategic project PEst-OE/AGR/UI0690/2011) and to PEst-OE/EQB/LA0016/2011. They also thank to CHTAD – Hospital Center of Trás-os-Montes e Alto Douro and Siemens for all the support.WileyBiblioteca Digital do IPBAlves, Maria JoséFerreira, Isabel C.F.R.Froufe, Hugo J.C.Abreu, Rui M.V.Martins, AnabelaPintado, Manuela2013-07-24T14:50:09Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/8556engAlves, Maria José; Ferreira, Isabel C.F.R.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Martins, Anabela; Pintado, Manuela (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology. ISSN 1365-2672. 115:2, p. 346–3571365-267210.1111/jam.12196info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:20:56Zoai:bibliotecadigital.ipb.pt:10198/8556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:00:02.888987Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
title |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
spellingShingle |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies Alves, Maria José Wild mushrooms Antimicrobial activity Clinical isolates SAR Docking |
title_short |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
title_full |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
title_fullStr |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
title_full_unstemmed |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
title_sort |
Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies |
author |
Alves, Maria José |
author_facet |
Alves, Maria José Ferreira, Isabel C.F.R. Froufe, Hugo J.C. Abreu, Rui M.V. Martins, Anabela Pintado, Manuela |
author_role |
author |
author2 |
Ferreira, Isabel C.F.R. Froufe, Hugo J.C. Abreu, Rui M.V. Martins, Anabela Pintado, Manuela |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Alves, Maria José Ferreira, Isabel C.F.R. Froufe, Hugo J.C. Abreu, Rui M.V. Martins, Anabela Pintado, Manuela |
dc.subject.por.fl_str_mv |
Wild mushrooms Antimicrobial activity Clinical isolates SAR Docking |
topic |
Wild mushrooms Antimicrobial activity Clinical isolates SAR Docking |
description |
Aim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-24T14:50:09Z 2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/8556 |
url |
http://hdl.handle.net/10198/8556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Alves, Maria José; Ferreira, Isabel C.F.R.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Martins, Anabela; Pintado, Manuela (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology. ISSN 1365-2672. 115:2, p. 346–357 1365-2672 10.1111/jam.12196 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135226496024576 |