Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies

Detalhes bibliográficos
Autor(a) principal: Alves, Maria José
Data de Publicação: 2013
Outros Autores: Ferreira, Isabel C.F.R., Froufe, Hugo J.C., Abreu, Rui M.V., Martins, Anabela, Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/8556
Resumo: Aim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics.
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spelling Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studiesWild mushroomsAntimicrobial activityClinical isolatesSARDockingAim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics.Fundação para a Ciência e a Tecnologia (FCT, Portugal) and COMPETE/QREN/EU for financial support to this work (research project PTDC/AGR-ALI/110062/2009) and to CIMO (strategic project PEst-OE/AGR/UI0690/2011) and to PEst-OE/EQB/LA0016/2011. They also thank to CHTAD – Hospital Center of Trás-os-Montes e Alto Douro and Siemens for all the support.WileyBiblioteca Digital do IPBAlves, Maria JoséFerreira, Isabel C.F.R.Froufe, Hugo J.C.Abreu, Rui M.V.Martins, AnabelaPintado, Manuela2013-07-24T14:50:09Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/8556engAlves, Maria José; Ferreira, Isabel C.F.R.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Martins, Anabela; Pintado, Manuela (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology. ISSN 1365-2672. 115:2, p. 346–3571365-267210.1111/jam.12196info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:20:56Zoai:bibliotecadigital.ipb.pt:10198/8556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:00:02.888987Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
title Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
spellingShingle Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
Alves, Maria José
Wild mushrooms
Antimicrobial activity
Clinical isolates
SAR
Docking
title_short Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
title_full Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
title_fullStr Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
title_full_unstemmed Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
title_sort Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies
author Alves, Maria José
author_facet Alves, Maria José
Ferreira, Isabel C.F.R.
Froufe, Hugo J.C.
Abreu, Rui M.V.
Martins, Anabela
Pintado, Manuela
author_role author
author2 Ferreira, Isabel C.F.R.
Froufe, Hugo J.C.
Abreu, Rui M.V.
Martins, Anabela
Pintado, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Alves, Maria José
Ferreira, Isabel C.F.R.
Froufe, Hugo J.C.
Abreu, Rui M.V.
Martins, Anabela
Pintado, Manuela
dc.subject.por.fl_str_mv Wild mushrooms
Antimicrobial activity
Clinical isolates
SAR
Docking
topic Wild mushrooms
Antimicrobial activity
Clinical isolates
SAR
Docking
description Aim and Methods: Although the antimicrobial activity of extracts from several mushroom species have been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights in the mechanism of action of potential antimicrobial drugs for resistant microorganisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram negative and Gram positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin sensible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs=0.5 mg/mL) and p-coumaric (MIC= 1 mg/mL) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin sensible Staphylococcus aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring, as also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some microorganisms resistant to commercial antibiotics.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-24T14:50:09Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/8556
url http://hdl.handle.net/10198/8556
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Maria José; Ferreira, Isabel C.F.R.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Martins, Anabela; Pintado, Manuela (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology. ISSN 1365-2672. 115:2, p. 346–357
1365-2672
10.1111/jam.12196
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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