Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://hdl.handle.net/1822/77801 |
Resumo: | "Available online 13 May 2022" |
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7160 |
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Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiaeTemperature toleranceTranscriptomeProteomeColdHeatSaccharomycesScience & Technology"Available online 13 May 2022"Understanding of thermal adaptation mechanisms in yeast is crucial to develop better-adapted strains to industrial processes, providing more economical and sustainable products. We have analyzed the transcriptomic responses of three Saccharomyces cerevisiae strains, a commercial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and a commercial bioethanol strain, Ethanol Red, grown at non-optimal temperatures under anaerobic chemostat conditions. Transcriptomic analysis of the three strains revealed a huge complexity of cellular mechanisms and responses. Overall, cold exerted a stronger transcriptional response in the three strains comparing with heat conditions, with a higher number of down-regulating genes than of up-regulating genes regardless the strain analyzed. The comparison of the transcriptome at both sub- and supra-optimal temperatures showed the presence of common genes up- or down-regulated in both conditions, but also the presence of common genes up- or down-regulated in the three studied strains. More specifically, we have identified and validated three up-regulated genes at sub-optimal temperature in the three strains, OPI3, EFM6 and YOL014W. Finally, the comparison of the transcriptomic data with a previous proteomic study with the same strains revealed a good correlation between gene activity and protein abundance, mainly at low temperature. Our work provides a global insight into the specific mechanisms involved in temperature adaptation regarding both transcriptome and proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.This work was supported by the Spanish Government through “Ministerio de Ciencia e Innovación” (MICINN) and “Fondo Europeo de Desarrollo Regional” (FEDER) funds (grant number PCIN-2015-143, PID2019-108722RB-C31) awarded to JMG and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. This study has been carried out in the context of the European Project ERA-IB “YeastTempTation".info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoGarcía-Ríos, EstéfaniAlonso-del-Real, JavierLip, Ka Ying FlorencePinheiro, T.Teixeira, J. A.Gulik, Walter vanDomingues, LucíliaQuerol, AmparoGuillamón, José Manuel2022-072022-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77801engGarcía-Ríos, Estéfani; Alonso-del-Real, Javier; Lip, Ka Ying Florence; Pinheiro, T.; Teixeira, José A.; Gulik, Walter van; Domingues, Lucília; Querol, Amparo; Guillamón, José Manuel, Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae. Genomics, 114(4), 110386, 20220888-754310.1016/j.ygeno.2022.11038635569731110386https://www.sciencedirect.com/journal/genomicsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:04Zoai:repositorium.sdum.uminho.pt:1822/77801Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:25.674066Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| title |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| spellingShingle |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae García-Ríos, Estéfani Temperature tolerance Transcriptome Proteome Cold Heat Saccharomyces Science & Technology |
| title_short |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| title_full |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| title_fullStr |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| title_full_unstemmed |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| title_sort |
Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae |
| author |
García-Ríos, Estéfani |
| author_facet |
García-Ríos, Estéfani Alonso-del-Real, Javier Lip, Ka Ying Florence Pinheiro, T. Teixeira, J. A. Gulik, Walter van Domingues, Lucília Querol, Amparo Guillamón, José Manuel |
| author_role |
author |
| author2 |
Alonso-del-Real, Javier Lip, Ka Ying Florence Pinheiro, T. Teixeira, J. A. Gulik, Walter van Domingues, Lucília Querol, Amparo Guillamón, José Manuel |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
García-Ríos, Estéfani Alonso-del-Real, Javier Lip, Ka Ying Florence Pinheiro, T. Teixeira, J. A. Gulik, Walter van Domingues, Lucília Querol, Amparo Guillamón, José Manuel |
| dc.subject.por.fl_str_mv |
Temperature tolerance Transcriptome Proteome Cold Heat Saccharomyces Science & Technology |
| topic |
Temperature tolerance Transcriptome Proteome Cold Heat Saccharomyces Science & Technology |
| description |
"Available online 13 May 2022" |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-07 2022-07-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/77801 |
| url |
https://hdl.handle.net/1822/77801 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
García-Ríos, Estéfani; Alonso-del-Real, Javier; Lip, Ka Ying Florence; Pinheiro, T.; Teixeira, José A.; Gulik, Walter van; Domingues, Lucília; Querol, Amparo; Guillamón, José Manuel, Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae. Genomics, 114(4), 110386, 2022 0888-7543 10.1016/j.ygeno.2022.110386 35569731 110386 https://www.sciencedirect.com/journal/genomics |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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