Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/6608 |
Resumo: | In vitro 3D cancer spheroids generally exhibit a drug resistance profile similar to that found in solid tumors. Due to this property, these models are an appealing for anticancer compounds screening. Nevertheless, the techniques and methods aimed for drug discovery are mostly standardized for cells cultured in 2D. The development of 2D cell culture models displaying a drug resistant profile is required to mimic the in vivo tumors, while the equipment, techniques, and methodologies established for conventional 2D cell cultures can continue to be employed in compound screening. In this work, the response of 3D‐derived MCF‐7 cells subsequently cultured in 2D in medium supplemented with glutathione (GSH) (antioxidant agent found in high levels in breast cancer tissues and a promoter of cancer cells resistance) to Doxorubicin (DOX) is evaluated. These cells demonstrated a resistance toward DOX closer to that displayed by 3D spheroids, which is higher than that exhibited by standard 2D cell cultures. In fact, the 50% inhibitory concentration (IC50) of DOX in 3D‐derived MCF‐7 cell cultures supplemented with GSH is about eight‐times higher than that obtained for conventional 2D cell cultures (cultured without GSH), and is only about two‐times lower than that attained for 3D MCF‐7 spheroids (cultured without GSH). Further investigation revealed that this improved resistance of 3D‐derived MCF‐7 cells may result from their increased P‐glycoprotein (P‐gp) activity and reduced production of intracellular reactive oxygen species (ROS). |
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Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile2D cell culturesBreast cancerDoxorubicinDrug resistanceGlutathioneSpheroidsIn vitro 3D cancer spheroids generally exhibit a drug resistance profile similar to that found in solid tumors. Due to this property, these models are an appealing for anticancer compounds screening. Nevertheless, the techniques and methods aimed for drug discovery are mostly standardized for cells cultured in 2D. The development of 2D cell culture models displaying a drug resistant profile is required to mimic the in vivo tumors, while the equipment, techniques, and methodologies established for conventional 2D cell cultures can continue to be employed in compound screening. In this work, the response of 3D‐derived MCF‐7 cells subsequently cultured in 2D in medium supplemented with glutathione (GSH) (antioxidant agent found in high levels in breast cancer tissues and a promoter of cancer cells resistance) to Doxorubicin (DOX) is evaluated. These cells demonstrated a resistance toward DOX closer to that displayed by 3D spheroids, which is higher than that exhibited by standard 2D cell cultures. In fact, the 50% inhibitory concentration (IC50) of DOX in 3D‐derived MCF‐7 cell cultures supplemented with GSH is about eight‐times higher than that obtained for conventional 2D cell cultures (cultured without GSH), and is only about two‐times lower than that attained for 3D MCF‐7 spheroids (cultured without GSH). Further investigation revealed that this improved resistance of 3D‐derived MCF‐7 cells may result from their increased P‐glycoprotein (P‐gp) activity and reduced production of intracellular reactive oxygen species (ROS).WileyuBibliorumNunes, Ana Raquel SantosCosta, Elisabete C.Barros, AndreiaDiogo, Duarte Miguel de MeloCorreia, Ilídio Joaquim Sobreira2018-12-11T10:04:17Z2018-092018-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/6608engNunes, A.S., Costa, E.C., Barros, A.S., de Melo-Diogo, D., Correia, I.J. (2018) "Establishment of 2D cell cultures derived from 3D MCF-7 spheroids displaying a Doxorubicin resistant profile", Biotechnology journal, pp. 1800268.10.1002/biot.201800268metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:45:17Zoai:ubibliorum.ubi.pt:10400.6/6608Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:47:17.658306Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
title |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
spellingShingle |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile Nunes, Ana Raquel Santos 2D cell cultures Breast cancer Doxorubicin Drug resistance Glutathione Spheroids |
title_short |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
title_full |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
title_fullStr |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
title_full_unstemmed |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
title_sort |
Establishment of 2D Cell Cultures Derived From 3D MCF‐7 Spheroids Displaying a Doxorubicin Resistant Profile |
author |
Nunes, Ana Raquel Santos |
author_facet |
Nunes, Ana Raquel Santos Costa, Elisabete C. Barros, Andreia Diogo, Duarte Miguel de Melo Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Costa, Elisabete C. Barros, Andreia Diogo, Duarte Miguel de Melo Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Nunes, Ana Raquel Santos Costa, Elisabete C. Barros, Andreia Diogo, Duarte Miguel de Melo Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
2D cell cultures Breast cancer Doxorubicin Drug resistance Glutathione Spheroids |
topic |
2D cell cultures Breast cancer Doxorubicin Drug resistance Glutathione Spheroids |
description |
In vitro 3D cancer spheroids generally exhibit a drug resistance profile similar to that found in solid tumors. Due to this property, these models are an appealing for anticancer compounds screening. Nevertheless, the techniques and methods aimed for drug discovery are mostly standardized for cells cultured in 2D. The development of 2D cell culture models displaying a drug resistant profile is required to mimic the in vivo tumors, while the equipment, techniques, and methodologies established for conventional 2D cell cultures can continue to be employed in compound screening. In this work, the response of 3D‐derived MCF‐7 cells subsequently cultured in 2D in medium supplemented with glutathione (GSH) (antioxidant agent found in high levels in breast cancer tissues and a promoter of cancer cells resistance) to Doxorubicin (DOX) is evaluated. These cells demonstrated a resistance toward DOX closer to that displayed by 3D spheroids, which is higher than that exhibited by standard 2D cell cultures. In fact, the 50% inhibitory concentration (IC50) of DOX in 3D‐derived MCF‐7 cell cultures supplemented with GSH is about eight‐times higher than that obtained for conventional 2D cell cultures (cultured without GSH), and is only about two‐times lower than that attained for 3D MCF‐7 spheroids (cultured without GSH). Further investigation revealed that this improved resistance of 3D‐derived MCF‐7 cells may result from their increased P‐glycoprotein (P‐gp) activity and reduced production of intracellular reactive oxygen species (ROS). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T10:04:17Z 2018-09 2018-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/6608 |
url |
http://hdl.handle.net/10400.6/6608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nunes, A.S., Costa, E.C., Barros, A.S., de Melo-Diogo, D., Correia, I.J. (2018) "Establishment of 2D cell cultures derived from 3D MCF-7 spheroids displaying a Doxorubicin resistant profile", Biotechnology journal, pp. 1800268. 10.1002/biot.201800268 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136368421502976 |