Application of Some Room Temperature Ionic Liquids in the Development of Biosensors at Carbon Film Electrodes

• Autor(a) principal: Pauliukaite, Rasa
• Data de Publicação: 2008
• Outros Autores: Doherty, Andrew P., Murnaghan, Kevin D., Brett, Christopher M. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10316/8257; https://doi.org/10.1002/elan.200704081

Two room temperature ionic liquids, 1-butyl-3-methylimidazolium bistriflimide and 1-butyl-3-methylimidazolium nitrate, were employed for enzyme immobilization in a new sol-gel matrix and, for the first time, were successfully applied as electrolyte carriers in a biosensing system. The new sol-gel matrix, based on 3-aminopropyltrimethoxysilane and 1-butyl-3-methylimidazolium bistriflimide mixtures, did not crack even after several weeks when kept dry, and exhibited similar analytical properties to aqueous sol-gel based glucose biosensors. The linear range was up to 1.1 mM of glucose, sensitivity was 62 nA mM-1 and the limit of detection was 28.8 muM. The optimum ionic liquid electrolyte carrier was found to be 1-butyl-3-methylimidazolium nitrate, where the biosensor was made by electrodeposition of the redox mediator, poly(neutral red), and the enzyme was immobilized by cross-linking with glutaraldehyde. The results showed that application of room temperature ionic liquids to biosensors is very promising and can be further exploited.