Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/111812 |
Resumo: | Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. Conversely, transplantation of wild-type bone marrow hematopoietic progenitors into Rag2-/-Il2rg-/- mice and consequent restoration of thymocyte-mediated TEC differentiation diminishes the frequency of colony-forming units within cTECs. Our findings provide evidence that the cortical epithelium contains a reservoir of epithelial progenitors whose abundance is dynamically controlled by continual interactions with developing thymocytes across lifespan. |
id |
RCAP_4c11170a4f6d691a9c298f5b89f64831 |
---|---|
oai_identifier_str |
oai:repositorio-aberto.up.pt:10216/111812 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor propertiesAnimalsCell DifferentiationClone CellsEpithelial Cells/cytologyEpithelial Cells/physiologyHumansLymphocyte ActivationMiceStem Cells/physiologyThymocytes/immunologyThymocytes/physiologyThymus Gland/cytologyThymus Gland/metabolismCortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. Conversely, transplantation of wild-type bone marrow hematopoietic progenitors into Rag2-/-Il2rg-/- mice and consequent restoration of thymocyte-mediated TEC differentiation diminishes the frequency of colony-forming units within cTECs. Our findings provide evidence that the cortical epithelium contains a reservoir of epithelial progenitors whose abundance is dynamically controlled by continual interactions with developing thymocytes across lifespan.Wiley-VCH2017-062017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10216/111812eng0014-298010.1002/eji.201746922Meireles, CRibeiro, ARPinto, RDLeitão, CRodrigues, PMAlves, NLinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:27:54Zoai:repositorio-aberto.up.pt:10216/111812Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:24:17.373867Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
title |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
spellingShingle |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties Meireles, C Animals Cell Differentiation Clone Cells Epithelial Cells/cytology Epithelial Cells/physiology Humans Lymphocyte Activation Mice Stem Cells/physiology Thymocytes/immunology Thymocytes/physiology Thymus Gland/cytology Thymus Gland/metabolism |
title_short |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
title_full |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
title_fullStr |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
title_full_unstemmed |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
title_sort |
Thymic crosstalk restrains the pool of cortical thymic epithelial cells with progenitor properties |
author |
Meireles, C |
author_facet |
Meireles, C Ribeiro, AR Pinto, RD Leitão, C Rodrigues, PM Alves, NL |
author_role |
author |
author2 |
Ribeiro, AR Pinto, RD Leitão, C Rodrigues, PM Alves, NL |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Meireles, C Ribeiro, AR Pinto, RD Leitão, C Rodrigues, PM Alves, NL |
dc.subject.por.fl_str_mv |
Animals Cell Differentiation Clone Cells Epithelial Cells/cytology Epithelial Cells/physiology Humans Lymphocyte Activation Mice Stem Cells/physiology Thymocytes/immunology Thymocytes/physiology Thymus Gland/cytology Thymus Gland/metabolism |
topic |
Animals Cell Differentiation Clone Cells Epithelial Cells/cytology Epithelial Cells/physiology Humans Lymphocyte Activation Mice Stem Cells/physiology Thymocytes/immunology Thymocytes/physiology Thymus Gland/cytology Thymus Gland/metabolism |
description |
Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. Conversely, transplantation of wild-type bone marrow hematopoietic progenitors into Rag2-/-Il2rg-/- mice and consequent restoration of thymocyte-mediated TEC differentiation diminishes the frequency of colony-forming units within cTECs. Our findings provide evidence that the cortical epithelium contains a reservoir of epithelial progenitors whose abundance is dynamically controlled by continual interactions with developing thymocytes across lifespan. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 2017-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/111812 |
url |
http://hdl.handle.net/10216/111812 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0014-2980 10.1002/eji.201746922 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-VCH |
publisher.none.fl_str_mv |
Wiley-VCH |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136157950279680 |