Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines

Detalhes bibliográficos
Autor(a) principal: Gamelas, Sara R. D.
Data de Publicação: 2023
Outros Autores: Bartolomeu, Maria, Gomes, Thierry J., Faustino, Maria A.F., Tomé, João P.C., Tomé, Augusto C., Almeida, Adelaide, Gomes, Ana T. P. C., Lourenço, Leandro M. O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/42770
Resumo: Among the various groups of microorganisms, viruses have generally a greater capacity for mutation, especially RNA viruses, as was demonstrated by SARS-CoV2 virus mutations. This high mutation rate promotes the development of their resistance to traditional antivirals and establishes the resistance behaviour in virus populations, decreasing their susceptibility to these drugs. In this context, the photodynamic treatment appears as a potentially effective method against microorganisms and, considering its mode of action is not likely to lead to the development of resistance. In this work, two newly zinc(II) phthalocyanines (ZnPcs) bearing pyridinium-pyrazolyl groups (2a and 3a) were synthesized, characterized, and applied in photodynamic inactivation (PDI) of bacteriophage Φ6 (or Phage Phi6) as a RNA-virus model. These quaternized dyes were applied at different concentrations (from 5.0 to 20 μM, and under white light irradiation in the irradiance range between 50 and 150 mW/cm2) to test their efficiency for possible clinical or environmental applications. The results showed that the new cationic ZnPcs 2a and 3a efficiently inactivate the RNA-virus model (bacteriophage Φ6), even at the lowest tested irradiance. These compounds are thus promising photosensitizers to be used in various contexts.
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spelling Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyaninesBacteriophage Φ6Photodynamic inactivation (PDI)PhthalocyaninesReactive oxygen species (ROS)RNA-VirusesSinglet oxygenAmong the various groups of microorganisms, viruses have generally a greater capacity for mutation, especially RNA viruses, as was demonstrated by SARS-CoV2 virus mutations. This high mutation rate promotes the development of their resistance to traditional antivirals and establishes the resistance behaviour in virus populations, decreasing their susceptibility to these drugs. In this context, the photodynamic treatment appears as a potentially effective method against microorganisms and, considering its mode of action is not likely to lead to the development of resistance. In this work, two newly zinc(II) phthalocyanines (ZnPcs) bearing pyridinium-pyrazolyl groups (2a and 3a) were synthesized, characterized, and applied in photodynamic inactivation (PDI) of bacteriophage Φ6 (or Phage Phi6) as a RNA-virus model. These quaternized dyes were applied at different concentrations (from 5.0 to 20 μM, and under white light irradiation in the irradiance range between 50 and 150 mW/cm2) to test their efficiency for possible clinical or environmental applications. The results showed that the new cationic ZnPcs 2a and 3a efficiently inactivate the RNA-virus model (bacteriophage Φ6), even at the lowest tested irradiance. These compounds are thus promising photosensitizers to be used in various contexts.Veritati - Repositório Institucional da Universidade Católica PortuguesaGamelas, Sara R. D.Bartolomeu, MariaGomes, Thierry J.Faustino, Maria A.F.Tomé, João P.C.Tomé, Augusto C.Almeida, AdelaideGomes, Ana T. P. C.Lourenço, Leandro M. O.2023-10-04T14:12:44Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42770eng0143-720810.1016/j.dyepig.2023.11166185171156370001082990600001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-31T01:33:39Zoai:repositorio.ucp.pt:10400.14/42770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:34:06.254203Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
title Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
spellingShingle Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
Gamelas, Sara R. D.
Bacteriophage Φ6
Photodynamic inactivation (PDI)
Phthalocyanines
Reactive oxygen species (ROS)
RNA-Viruses
Singlet oxygen
title_short Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
title_full Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
title_fullStr Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
title_full_unstemmed Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
title_sort Photodynamic inactivation of a RNA-virus model using water-soluble β-octa-Substituted pyridinium-pyrazolyl phthalocyanines
author Gamelas, Sara R. D.
author_facet Gamelas, Sara R. D.
Bartolomeu, Maria
Gomes, Thierry J.
Faustino, Maria A.F.
Tomé, João P.C.
Tomé, Augusto C.
Almeida, Adelaide
Gomes, Ana T. P. C.
Lourenço, Leandro M. O.
author_role author
author2 Bartolomeu, Maria
Gomes, Thierry J.
Faustino, Maria A.F.
Tomé, João P.C.
Tomé, Augusto C.
Almeida, Adelaide
Gomes, Ana T. P. C.
Lourenço, Leandro M. O.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Gamelas, Sara R. D.
Bartolomeu, Maria
Gomes, Thierry J.
Faustino, Maria A.F.
Tomé, João P.C.
Tomé, Augusto C.
Almeida, Adelaide
Gomes, Ana T. P. C.
Lourenço, Leandro M. O.
dc.subject.por.fl_str_mv Bacteriophage Φ6
Photodynamic inactivation (PDI)
Phthalocyanines
Reactive oxygen species (ROS)
RNA-Viruses
Singlet oxygen
topic Bacteriophage Φ6
Photodynamic inactivation (PDI)
Phthalocyanines
Reactive oxygen species (ROS)
RNA-Viruses
Singlet oxygen
description Among the various groups of microorganisms, viruses have generally a greater capacity for mutation, especially RNA viruses, as was demonstrated by SARS-CoV2 virus mutations. This high mutation rate promotes the development of their resistance to traditional antivirals and establishes the resistance behaviour in virus populations, decreasing their susceptibility to these drugs. In this context, the photodynamic treatment appears as a potentially effective method against microorganisms and, considering its mode of action is not likely to lead to the development of resistance. In this work, two newly zinc(II) phthalocyanines (ZnPcs) bearing pyridinium-pyrazolyl groups (2a and 3a) were synthesized, characterized, and applied in photodynamic inactivation (PDI) of bacteriophage Φ6 (or Phage Phi6) as a RNA-virus model. These quaternized dyes were applied at different concentrations (from 5.0 to 20 μM, and under white light irradiation in the irradiance range between 50 and 150 mW/cm2) to test their efficiency for possible clinical or environmental applications. The results showed that the new cationic ZnPcs 2a and 3a efficiently inactivate the RNA-virus model (bacteriophage Φ6), even at the lowest tested irradiance. These compounds are thus promising photosensitizers to be used in various contexts.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-04T14:12:44Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/42770
url http://hdl.handle.net/10400.14/42770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0143-7208
10.1016/j.dyepig.2023.111661
85171156370
001082990600001
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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