Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.26/14802 |
Resumo: | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| id |
RCAP_4ca38acd4aaea4d9b902be29e2cb54c1 |
|---|---|
| oai_identifier_str |
oai:comum.rcaap.pt:10400.26/14802 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solventsDownstream processingCell disruptionSeparationPurificationPoly(3-hydroxybutyrate) recoveryAnisoleCyclohexanone© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"A technique using safer, non-chlorinated organic solvents for the extraction of poly-3-hydroxybutyrate (P(3HB)) from bacterial cells was developed, aiming to attain high recovery yields and purities. Some solvents were selected from the GlaxoSmithKline guide as sustainable industrial solvents and the solubility of P(3HB) calculated using predictive equations from literature. Based on the calculated solubility values, anisole, cyclohexanone and phenetole were tested as extraction solvents and the relevant process variables (extraction temperature, extraction time and mass of cells/solvent volume ratio) were addressed. Polymer recovery yields of 97% and 93% were obtained with anisole and cyclohexanone, respectively, at 120–130 °C using a cell/solvent ratio of 1.5% (w/v). Maximum polymer purities using these experimental conditions were 98% for both solvents. The recovery yield and the polymer purity attained with chloroform (reference solvent) were 96 and 98%, respectively. Higher cell/solvent ratios of 6.0% (w/v) showed slightly lower recovery yields and purities. The average molecular weight and the thermal properties of the polymers extracted with the alternative solvents were fully comparable to those of the polymers obtained by chloroform extraction, demonstrating that the applied conditions did not significantly alter the properties of the extracted P(3HB)."ElsevierRepositório ComumRosengart, AlessandroCesário, M.TeresaAlmeida, M. Catarina M.D. deRaposo, Rodrigo S.Espert, AnaDíaz de Apodaca, ElenaFonseca, M. Manuela R. da2016-10-31T01:30:11Z2015-11-01T00:00:00Z2015-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.26/14802engBiochemical Engineering Journal Volume 103, 15 November 2015, Pages 39–461369-703X10.1016/j.bej.2015.06.013info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:52:29Zoai:comum.rcaap.pt:10400.26/14802Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:08:21.451229Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| title |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| spellingShingle |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents Rosengart, Alessandro Downstream processing Cell disruption Separation Purification Poly(3-hydroxybutyrate) recovery Anisole Cyclohexanone |
| title_short |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| title_full |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| title_fullStr |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| title_full_unstemmed |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| title_sort |
Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents |
| author |
Rosengart, Alessandro |
| author_facet |
Rosengart, Alessandro Cesário, M.Teresa Almeida, M. Catarina M.D. de Raposo, Rodrigo S. Espert, Ana Díaz de Apodaca, Elena Fonseca, M. Manuela R. da |
| author_role |
author |
| author2 |
Cesário, M.Teresa Almeida, M. Catarina M.D. de Raposo, Rodrigo S. Espert, Ana Díaz de Apodaca, Elena Fonseca, M. Manuela R. da |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Comum |
| dc.contributor.author.fl_str_mv |
Rosengart, Alessandro Cesário, M.Teresa Almeida, M. Catarina M.D. de Raposo, Rodrigo S. Espert, Ana Díaz de Apodaca, Elena Fonseca, M. Manuela R. da |
| dc.subject.por.fl_str_mv |
Downstream processing Cell disruption Separation Purification Poly(3-hydroxybutyrate) recovery Anisole Cyclohexanone |
| topic |
Downstream processing Cell disruption Separation Purification Poly(3-hydroxybutyrate) recovery Anisole Cyclohexanone |
| description |
© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-11-01T00:00:00Z 2015-11-01T00:00:00Z 2016-10-31T01:30:11Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/14802 |
| url |
http://hdl.handle.net/10400.26/14802 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Biochemical Engineering Journal Volume 103, 15 November 2015, Pages 39–46 1369-703X 10.1016/j.bej.2015.06.013 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799129951733022720 |