Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections

Detalhes bibliográficos
Autor(a) principal: Asgarifar, Sanaz
Data de Publicação: 2019
Outros Autores: Mestre, Ana L. G., Félix, Rute, Inacio, Pedro M. C., Lurdes S. Cristiano, M., Medeiros, Maria C. R., Araújo, Inês, Power, Deborah, Gomes, Henrique L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/14164
Resumo: Herein, we describe an electrophysiological based sensor that reproducibly monitors and quantifies in real-time collective migration and the formation of cell-cell junctions by C6 glioma cells seeded on top of electrodes. The signal amplitude and frequency generated by the migrating cells changed over time and these parameters were used to accurately calculate the migration speed. Electrophysiological measurements could also distinguish individual from collective cell migration. The migration of densely packed cells generated strong signals, while dispersed cells showed weak bioelectrical activity. We propose this electrophysiological technique as a cell-based biosensor to gain insight into the mechanisms of cooperative migration of cancer cells. Possible applications include screening for anti-migratory compounds, which may lead to the development of novel strategies for antineoplastic chemotherapy.
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spelling Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connectionsIon channelsOscillationsBiosensorArraysWavesHerein, we describe an electrophysiological based sensor that reproducibly monitors and quantifies in real-time collective migration and the formation of cell-cell junctions by C6 glioma cells seeded on top of electrodes. The signal amplitude and frequency generated by the migrating cells changed over time and these parameters were used to accurately calculate the migration speed. Electrophysiological measurements could also distinguish individual from collective cell migration. The migration of densely packed cells generated strong signals, while dispersed cells showed weak bioelectrical activity. We propose this electrophysiological technique as a cell-based biosensor to gain insight into the mechanisms of cooperative migration of cancer cells. Possible applications include screening for anti-migratory compounds, which may lead to the development of novel strategies for antineoplastic chemotherapy.Portuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [PTDC/EEI-AUT/5442/2014, UID/EEA/50008/2019, UID/BIM/04773/2019, UID/Multi/04326/2019]Universidade do AlgarveElsevier Advanced TechnologySapientiaAsgarifar, SanazMestre, Ana L. G.Félix, RuteInacio, Pedro M. C.Lurdes S. Cristiano, M.Medeiros, Maria C. R.Araújo, InêsPower, DeborahGomes, Henrique L.2021-12-01T01:30:15Z2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14164eng0956-566310.1016/j.bios.2019.111708info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:25Zoai:sapientia.ualg.pt:10400.1/14164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:12.953840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
title Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
spellingShingle Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
Asgarifar, Sanaz
Ion channels
Oscillations
Biosensor
Arrays
Waves
title_short Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
title_full Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
title_fullStr Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
title_full_unstemmed Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
title_sort Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
author Asgarifar, Sanaz
author_facet Asgarifar, Sanaz
Mestre, Ana L. G.
Félix, Rute
Inacio, Pedro M. C.
Lurdes S. Cristiano, M.
Medeiros, Maria C. R.
Araújo, Inês
Power, Deborah
Gomes, Henrique L.
author_role author
author2 Mestre, Ana L. G.
Félix, Rute
Inacio, Pedro M. C.
Lurdes S. Cristiano, M.
Medeiros, Maria C. R.
Araújo, Inês
Power, Deborah
Gomes, Henrique L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Asgarifar, Sanaz
Mestre, Ana L. G.
Félix, Rute
Inacio, Pedro M. C.
Lurdes S. Cristiano, M.
Medeiros, Maria C. R.
Araújo, Inês
Power, Deborah
Gomes, Henrique L.
dc.subject.por.fl_str_mv Ion channels
Oscillations
Biosensor
Arrays
Waves
topic Ion channels
Oscillations
Biosensor
Arrays
Waves
description Herein, we describe an electrophysiological based sensor that reproducibly monitors and quantifies in real-time collective migration and the formation of cell-cell junctions by C6 glioma cells seeded on top of electrodes. The signal amplitude and frequency generated by the migrating cells changed over time and these parameters were used to accurately calculate the migration speed. Electrophysiological measurements could also distinguish individual from collective cell migration. The migration of densely packed cells generated strong signals, while dispersed cells showed weak bioelectrical activity. We propose this electrophysiological technique as a cell-based biosensor to gain insight into the mechanisms of cooperative migration of cancer cells. Possible applications include screening for anti-migratory compounds, which may lead to the development of novel strategies for antineoplastic chemotherapy.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2019-12-01T00:00:00Z
2021-12-01T01:30:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/14164
url http://hdl.handle.net/10400.1/14164
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0956-5663
10.1016/j.bios.2019.111708
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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