Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci

Detalhes bibliográficos
Autor(a) principal: Caperta, A.
Data de Publicação: 2002
Outros Autores: Neves, Nuno, Morais-Cecilio, L., Malhó, Rui, Viegas, Wanda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/2844
Resumo: Research Article
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spelling Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA locihomologous gene expressionrDNA topologychromosome structuretelocentric chromosomesryeResearch ArticleThe standard rye cultivar ‘Imperial’ and a structural variant carrying an intact 1R chromosome and two telocentric 1R chromosomes (short and long arms) were used to investigate expression patterns of homologous rDNA loci, and the influence of chromosome structural change on their interphase organisation and relative disposition. Sequential silver staining and in situ hybridization with the rDNA probe pTa71, established a correspondence between the expression and organization patterns of rDNA domains in metaphase and interphase cells. In most cells of the cultivar Imperial, nucleolar organizer region (NOR) silver staining on metaphase chromosomes with equivalent numbers of rDNA genes revealed a size heteromorphism between homologousrDNA loci, resulting from their differential expression. NOR heteromorphism in the structural variant line was significantly reduced. The preferential activity of one NOR over its homologue was found to be random within cells and independent of parental origin. Nucleotypic modifications mediated by changes in the 1R chromosome structure include increased proximity between homologous rDNA loci in interphase, and an increase in the frequency of cells with intra-nucleolar ribosomal condensed chromatin. These results seem to indicate a ‘sequence recognition’ process for the regulation of homologous loci.The Company of BiologistsRepositório da Universidade de LisboaCaperta, A.Neves, NunoMorais-Cecilio, L.Malhó, RuiViegas, Wanda2011-01-24T14:36:44Z20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/2844eng"Journal of Cell Science". 115 (2002) 2839-2846info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-15T01:32:13Zoai:www.repository.utl.pt:10400.5/2844Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:50:49.202837Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
title Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
spellingShingle Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
Caperta, A.
homologous gene expression
rDNA topology
chromosome structure
telocentric chromosomes
rye
title_short Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
title_full Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
title_fullStr Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
title_full_unstemmed Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
title_sort Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci
author Caperta, A.
author_facet Caperta, A.
Neves, Nuno
Morais-Cecilio, L.
Malhó, Rui
Viegas, Wanda
author_role author
author2 Neves, Nuno
Morais-Cecilio, L.
Malhó, Rui
Viegas, Wanda
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Caperta, A.
Neves, Nuno
Morais-Cecilio, L.
Malhó, Rui
Viegas, Wanda
dc.subject.por.fl_str_mv homologous gene expression
rDNA topology
chromosome structure
telocentric chromosomes
rye
topic homologous gene expression
rDNA topology
chromosome structure
telocentric chromosomes
rye
description Research Article
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
2011-01-24T14:36:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/2844
url http://hdl.handle.net/10400.5/2844
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Cell Science". 115 (2002) 2839-2846
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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