Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.24/1770 |
Resumo: | Muscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation. |
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Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expressionAdultAgedAged, 80 and overAmino AcidsBicarbonatesCombined Modality TherapyFemaleHumansLactic AcidMaleMiddle AgedMuscle ProteinsMuscle, SkeletalProto-Oncogene Proteins c-aktRenal Insufficiency, ChronicTripartite Motif ProteinsUbiquitin-Protein LigasesExercise TherapyWalkingMuscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation.Repositório Científico da UMAIAWatson, Emma L.Kosmadakis, George C.Smith, Alice C.Viana, Joao L.Brown, Jeremy R.Molyneux, KarenPawluczyk, Izabella Z. A.Mulheran, MichaelBishop, Nicolette C.Shirreffs, SusanMaughan, Ronald J.Owen, Paul J.John, Stephen G.McIntyre, Christopher W.Feehally, JohnBevington, Alan2021-04-28T14:41:06Z2013-01-01T00:00:00Z2013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://hdl.handle.net/10400.24/1770eng10.1007/s00421-013-2628-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-26T16:01:08Zoai:repositorio.umaia.pt:10400.24/1770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:10:07.805638Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
title |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
spellingShingle |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression Watson, Emma L. Adult Aged Aged, 80 and over Amino Acids Bicarbonates Combined Modality Therapy Female Humans Lactic Acid Male Middle Aged Muscle Proteins Muscle, Skeletal Proto-Oncogene Proteins c-akt Renal Insufficiency, Chronic Tripartite Motif Proteins Ubiquitin-Protein Ligases Exercise Therapy Walking |
title_short |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
title_full |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
title_fullStr |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
title_full_unstemmed |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
title_sort |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression |
author |
Watson, Emma L. |
author_facet |
Watson, Emma L. Kosmadakis, George C. Smith, Alice C. Viana, Joao L. Brown, Jeremy R. Molyneux, Karen Pawluczyk, Izabella Z. A. Mulheran, Michael Bishop, Nicolette C. Shirreffs, Susan Maughan, Ronald J. Owen, Paul J. John, Stephen G. McIntyre, Christopher W. Feehally, John Bevington, Alan |
author_role |
author |
author2 |
Kosmadakis, George C. Smith, Alice C. Viana, Joao L. Brown, Jeremy R. Molyneux, Karen Pawluczyk, Izabella Z. A. Mulheran, Michael Bishop, Nicolette C. Shirreffs, Susan Maughan, Ronald J. Owen, Paul J. John, Stephen G. McIntyre, Christopher W. Feehally, John Bevington, Alan |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico da UMAIA |
dc.contributor.author.fl_str_mv |
Watson, Emma L. Kosmadakis, George C. Smith, Alice C. Viana, Joao L. Brown, Jeremy R. Molyneux, Karen Pawluczyk, Izabella Z. A. Mulheran, Michael Bishop, Nicolette C. Shirreffs, Susan Maughan, Ronald J. Owen, Paul J. John, Stephen G. McIntyre, Christopher W. Feehally, John Bevington, Alan |
dc.subject.por.fl_str_mv |
Adult Aged Aged, 80 and over Amino Acids Bicarbonates Combined Modality Therapy Female Humans Lactic Acid Male Middle Aged Muscle Proteins Muscle, Skeletal Proto-Oncogene Proteins c-akt Renal Insufficiency, Chronic Tripartite Motif Proteins Ubiquitin-Protein Ligases Exercise Therapy Walking |
topic |
Adult Aged Aged, 80 and over Amino Acids Bicarbonates Combined Modality Therapy Female Humans Lactic Acid Male Middle Aged Muscle Proteins Muscle, Skeletal Proto-Oncogene Proteins c-akt Renal Insufficiency, Chronic Tripartite Motif Proteins Ubiquitin-Protein Ligases Exercise Therapy Walking |
description |
Muscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01T00:00:00Z 2013-01-01T00:00:00Z 2021-04-28T14:41:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.24/1770 |
url |
http://hdl.handle.net/10400.24/1770 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1007/s00421-013-2628-5 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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