A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/3315 |
Resumo: | It has been postulated that a decrease in Na,KATPase-mediated ion gradients may be a contributing mechanism to insulin secretion. However, the precise role of the Na,K-ATPase in pancreatic β-cell membrane depolarization and insulin secretion signalling have been difficult to evaluate, mostly because data reporting changes in enzymatic activity have been obtained in cell homogenates or membrane preparations, lacking intact intracellular signalling pathways. The aim of this work was to develop a method to characterize Na,K-ATPase activity in intact pancreatic β-cells that will allow the investigation of putative Na,K-ATPase activity regulation by glucose and its possible role in insulin secretion signalling. This work demonstrates for the first time that it is possible to determine Na,K-ATPase activity in intact pancreatic βcells and that this is a suitable method for the study of the mechanisms involved in the Na,K-ATPase regulation and eventually its relevance for insulin secretion signalling. |
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A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cellsNa,K-ATPasePancreatic b-cellIt has been postulated that a decrease in Na,KATPase-mediated ion gradients may be a contributing mechanism to insulin secretion. However, the precise role of the Na,K-ATPase in pancreatic β-cell membrane depolarization and insulin secretion signalling have been difficult to evaluate, mostly because data reporting changes in enzymatic activity have been obtained in cell homogenates or membrane preparations, lacking intact intracellular signalling pathways. The aim of this work was to develop a method to characterize Na,K-ATPase activity in intact pancreatic β-cells that will allow the investigation of putative Na,K-ATPase activity regulation by glucose and its possible role in insulin secretion signalling. This work demonstrates for the first time that it is possible to determine Na,K-ATPase activity in intact pancreatic βcells and that this is a suitable method for the study of the mechanisms involved in the Na,K-ATPase regulation and eventually its relevance for insulin secretion signalling.Springer2012-01-11T14:37:36Z2012-01-112010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/3315http://hdl.handle.net/10174/3315porCosta, Ana R.; Real, Joana; Antunes, Célia M.; Cruz-Morais, Júlio. A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells, In Vitro Cellular & Developmental Biology - Animal, 46, 1, 7-10, 2010.1071-2690acrc@uevora.ptcucareal@gmail.comcmma@uevora.ptcmorais@uevora.pt365Costa, Ana R.Real, JoanaAntunes, Célia M.Cruz-Morais, Júlioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:40:19Zoai:dspace.uevora.pt:10174/3315Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:58:45.338618Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
title |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
spellingShingle |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells Costa, Ana R. Na,K-ATPase Pancreatic b-cell |
title_short |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
title_full |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
title_fullStr |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
title_full_unstemmed |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
title_sort |
A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells |
author |
Costa, Ana R. |
author_facet |
Costa, Ana R. Real, Joana Antunes, Célia M. Cruz-Morais, Júlio |
author_role |
author |
author2 |
Real, Joana Antunes, Célia M. Cruz-Morais, Júlio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Costa, Ana R. Real, Joana Antunes, Célia M. Cruz-Morais, Júlio |
dc.subject.por.fl_str_mv |
Na,K-ATPase Pancreatic b-cell |
topic |
Na,K-ATPase Pancreatic b-cell |
description |
It has been postulated that a decrease in Na,KATPase-mediated ion gradients may be a contributing mechanism to insulin secretion. However, the precise role of the Na,K-ATPase in pancreatic β-cell membrane depolarization and insulin secretion signalling have been difficult to evaluate, mostly because data reporting changes in enzymatic activity have been obtained in cell homogenates or membrane preparations, lacking intact intracellular signalling pathways. The aim of this work was to develop a method to characterize Na,K-ATPase activity in intact pancreatic β-cells that will allow the investigation of putative Na,K-ATPase activity regulation by glucose and its possible role in insulin secretion signalling. This work demonstrates for the first time that it is possible to determine Na,K-ATPase activity in intact pancreatic βcells and that this is a suitable method for the study of the mechanisms involved in the Na,K-ATPase regulation and eventually its relevance for insulin secretion signalling. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01T00:00:00Z 2012-01-11T14:37:36Z 2012-01-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/3315 http://hdl.handle.net/10174/3315 |
url |
http://hdl.handle.net/10174/3315 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Costa, Ana R.; Real, Joana; Antunes, Célia M.; Cruz-Morais, Júlio. A new approach for determination of Na,K-ATPase activity: application to intact pancreatic ß-cells, In Vitro Cellular & Developmental Biology - Animal, 46, 1, 7-10, 2010. 1071-2690 acrc@uevora.pt cucareal@gmail.com cmma@uevora.pt cmorais@uevora.pt 365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136470911418368 |