Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/40437 |
Resumo: | Purpose Congenital diaphragmatic hernia (CDH) is characterized by a developmental defect in the diaphragm, pulmonary hypoplasia and pulmonary hypertension. NPAS3 is a PAS domain transcription factor regulating Drosophila tracheogenesis. NPAS3 null mice develop pulmonary hypoplasia in utero and die after birth due to respiratory failure. We aimed to evaluate NPAS3 expres- sion during normal and abnormal lung development due to CDH. Methods CDH was induced by administering 100 mg/ml nitrofen to time-pregnant dams on embryonic day (E) 9 of gestation. Lungs were isolated on E15, E18 and E21 and NPAS3 localization was determined by immunohisto- chemistry and quantified using Western blotting. Results We found that only E21 hypoplastic CDH lungs have reduced expression of NPAS3 in the terminal sac- cules. Western blotting confirmed the down-regulation of NPAS3 protein in the nitrofen-induced hypoplastic lungs. Conclusions We demonstrate for the first time that ni- trofen-induced hypoplastic CDH lungs have reduced NPAS3 expression in the terminal saccules during the later stages of abnormal lung development. Our findings suggest that NPAS3 is associated with pulmonary hypoplasia in CDH. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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spelling |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic herniaCongenital diaphragmatic herniaPulmonary hypoplasiaLung developmentNitrofenNPAS3Ciências Médicas::Medicina ClínicaPurpose Congenital diaphragmatic hernia (CDH) is characterized by a developmental defect in the diaphragm, pulmonary hypoplasia and pulmonary hypertension. NPAS3 is a PAS domain transcription factor regulating Drosophila tracheogenesis. NPAS3 null mice develop pulmonary hypoplasia in utero and die after birth due to respiratory failure. We aimed to evaluate NPAS3 expres- sion during normal and abnormal lung development due to CDH. Methods CDH was induced by administering 100 mg/ml nitrofen to time-pregnant dams on embryonic day (E) 9 of gestation. Lungs were isolated on E15, E18 and E21 and NPAS3 localization was determined by immunohisto- chemistry and quantified using Western blotting. Results We found that only E21 hypoplastic CDH lungs have reduced expression of NPAS3 in the terminal sac- cules. Western blotting confirmed the down-regulation of NPAS3 protein in the nitrofen-induced hypoplastic lungs. Conclusions We demonstrate for the first time that ni- trofen-induced hypoplastic CDH lungs have reduced NPAS3 expression in the terminal saccules during the later stages of abnormal lung development. Our findings suggest that NPAS3 is associated with pulmonary hypoplasia in CDH.Supported by the Children’s Hospital Research Institute of Manitoba; RK is the recipient of a Career Enhancement Award from the Canadian Child Health Clinician Scientist Program and a New Investigator Salary Award from the Canadian Institutes of Health Research, Manitoba Lung Association and the Children’s Hospital Research Institute.Springer VerlagUniversidade do MinhoTerra, Patrícia Daniela PereiraKholdebarin, RaminHiggins, MeghanIwasiow, Barbara M.Correia-Pinto, JorgeKeijzer, Richard2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40437engPereira-Terra, P., Kholdebarin, R., Higgins, M., et. al. (2015). Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia. Pediatric surgery international, 31(7), 659-663.0179-035810.1007/s00383-015-3703-2http://link.springer.com/article/10.1007/s00383-015-3703-2#/page-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:19Zoai:repositorium.sdum.uminho.pt:1822/40437Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:17.945416Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
title |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
spellingShingle |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia Terra, Patrícia Daniela Pereira Congenital diaphragmatic hernia Pulmonary hypoplasia Lung development Nitrofen NPAS3 Ciências Médicas::Medicina Clínica |
title_short |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
title_full |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
title_fullStr |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
title_full_unstemmed |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
title_sort |
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia |
author |
Terra, Patrícia Daniela Pereira |
author_facet |
Terra, Patrícia Daniela Pereira Kholdebarin, Ramin Higgins, Meghan Iwasiow, Barbara M. Correia-Pinto, Jorge Keijzer, Richard |
author_role |
author |
author2 |
Kholdebarin, Ramin Higgins, Meghan Iwasiow, Barbara M. Correia-Pinto, Jorge Keijzer, Richard |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Terra, Patrícia Daniela Pereira Kholdebarin, Ramin Higgins, Meghan Iwasiow, Barbara M. Correia-Pinto, Jorge Keijzer, Richard |
dc.subject.por.fl_str_mv |
Congenital diaphragmatic hernia Pulmonary hypoplasia Lung development Nitrofen NPAS3 Ciências Médicas::Medicina Clínica |
topic |
Congenital diaphragmatic hernia Pulmonary hypoplasia Lung development Nitrofen NPAS3 Ciências Médicas::Medicina Clínica |
description |
Purpose Congenital diaphragmatic hernia (CDH) is characterized by a developmental defect in the diaphragm, pulmonary hypoplasia and pulmonary hypertension. NPAS3 is a PAS domain transcription factor regulating Drosophila tracheogenesis. NPAS3 null mice develop pulmonary hypoplasia in utero and die after birth due to respiratory failure. We aimed to evaluate NPAS3 expres- sion during normal and abnormal lung development due to CDH. Methods CDH was induced by administering 100 mg/ml nitrofen to time-pregnant dams on embryonic day (E) 9 of gestation. Lungs were isolated on E15, E18 and E21 and NPAS3 localization was determined by immunohisto- chemistry and quantified using Western blotting. Results We found that only E21 hypoplastic CDH lungs have reduced expression of NPAS3 in the terminal sac- cules. Western blotting confirmed the down-regulation of NPAS3 protein in the nitrofen-induced hypoplastic lungs. Conclusions We demonstrate for the first time that ni- trofen-induced hypoplastic CDH lungs have reduced NPAS3 expression in the terminal saccules during the later stages of abnormal lung development. Our findings suggest that NPAS3 is associated with pulmonary hypoplasia in CDH. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 2015-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/40437 |
url |
http://hdl.handle.net/1822/40437 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira-Terra, P., Kholdebarin, R., Higgins, M., et. al. (2015). Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia. Pediatric surgery international, 31(7), 659-663. 0179-0358 10.1007/s00383-015-3703-2 http://link.springer.com/article/10.1007/s00383-015-3703-2#/page-1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133002765172736 |