Progesterone modulates Candida albicans biofilm formation / expression

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Bruna
Data de Publicação: 2017
Outros Autores: Bernardo, Ruben, Wang, Can, Butler, Geraldine, Azeredo, Joana, Henriques, Mariana, Mira, Nuno, Silva, Sónia Carina
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/47182
Resumo: EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms
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spelling Progesterone modulates Candida albicans biofilm formation / expressionCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialEUROBIOFILMS 2017 - 5th European Congress on Microbial BiofilmsVulvovaginal candidiasis (VVC) caused by Candida albicans is a common disease worldwide. The most important C. albicans virulence factor is its ability to form biofilms, which, in the vaginal environment, may be formed on the epithelium and on intrauterine devices. Despite it has been shown that VVC has a hormonal dependency, the effects of progesterone on biofilm formation by C. albicans are still poorly understood. Thus, this work aimed to deepen the knowledge in that field by studying the effect of progesterone on C. albicans biofilm formation. C. albicans biofilms were formed in the presence and absence of progesterone and evaluated in terms of cell cultivability, total biomass, metabolic activity, structure, matrix composition and also genomic expression using species-specific microarrays. The results showed that progesterone reduced the ability of C. albicans to form biofilms, decreasing their cell cultivability, biomass, structural cohesion, matrix production and matrix carbohydrate content. Accordingly, biofilms formed in the presence of progesterone presented lower expression of several genes involved in C. albicans adhesion and biofilm formation (e.g. TEC1, BRG1, PBR1, AHR1 and HSP104) than biofilms formed in hormone-free medium. Genes involved in metabolism of carbohydrates were also found to have a reduced expression in biofilms formed with progesterone. On the other hand, progesterone presence led to an over-expression of genes involved in external stimulus response, such as those encoding drug-transporters (e.g. CDR1 and CDR2), and also in lipid metabolism (PXP2, POT1, FAT1, ANT1, MLS1, ICL1 and CIT1). Overall, the results of this study show that progesterone has a role in the modulation of C. albicans biofilm formation, which may have implications on C. albicans pathogenicity in the vaginal environment.Portuguese Foundation for Science and Technology (FCT), COMPETE 2020 and BioTecNort operationinfo:eu-repo/semantics/publishedVersionEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID)Universidade do MinhoGonçalves, BrunaBernardo, RubenWang, CanButler, GeraldineAzeredo, JoanaHenriques, MarianaMira, NunoSilva, Sónia Carina2017-09-192017-09-19T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/47182engGonçalves, Bruna; Bernardo, Ruben; Wang, Can; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno; Silva, Sónia Carina, Progesterone modulates Candida albicans biofilm formation / expression. EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms. No. 037, Amesterdam, Netherlands, 19-22 September, 109, 2017.http://www.eurobiofilms.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:43:44Zoai:repositorium.sdum.uminho.pt:1822/47182Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:43:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Progesterone modulates Candida albicans biofilm formation / expression
title Progesterone modulates Candida albicans biofilm formation / expression
spellingShingle Progesterone modulates Candida albicans biofilm formation / expression
Gonçalves, Bruna
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
title_short Progesterone modulates Candida albicans biofilm formation / expression
title_full Progesterone modulates Candida albicans biofilm formation / expression
title_fullStr Progesterone modulates Candida albicans biofilm formation / expression
title_full_unstemmed Progesterone modulates Candida albicans biofilm formation / expression
title_sort Progesterone modulates Candida albicans biofilm formation / expression
author Gonçalves, Bruna
author_facet Gonçalves, Bruna
Bernardo, Ruben
Wang, Can
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno
Silva, Sónia Carina
author_role author
author2 Bernardo, Ruben
Wang, Can
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno
Silva, Sónia Carina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, Bruna
Bernardo, Ruben
Wang, Can
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno
Silva, Sónia Carina
dc.subject.por.fl_str_mv Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
topic Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
description EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms
publishDate 2017
dc.date.none.fl_str_mv 2017-09-19
2017-09-19T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/47182
url http://hdl.handle.net/1822/47182
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gonçalves, Bruna; Bernardo, Ruben; Wang, Can; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno; Silva, Sónia Carina, Progesterone modulates Candida albicans biofilm formation / expression. EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms. No. 037, Amesterdam, Netherlands, 19-22 September, 109, 2017.
http://www.eurobiofilms.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
publisher.none.fl_str_mv European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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