The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/37259 |
Resumo: | Endolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of the reproduction cycle of bacteriophages, have great potential to control Gram-pos- itive pathogens. This work describes the characterization of a novel endolysin (PlyPl23) encoded on the genome of Paenibacillus larvae phage phiIBB_Pl23 with high potential to control American foulbrood. This bacterial disease, caused by P. larvae, is widespread in North America and Europe and causes important economic losses in apiculture. The restric- tion to antibiotic residues in honey imposed by the EU legislation hinders its therapeutic use to combat American foulbrood and enforces the development of alternative antimicrobial methods. The new endolysin described herein has an N-acetylmuramoyl-L-alanine ami- dase catalytic domain and exhibits a broad-spectrum activity against common P. larvae genotypes. Moreover, the enzyme displays high antimicrobial activity in a range of pH that matches environmental conditions (pH between 5.0 and 7.0), showing its feasible applica- tion in the field. At pH 7.0, a concentration of 0.2 M of enzyme was enough to lyse 104 CFU.mL-1 of P. larvae in no more than 2 h. The presence of sucrose and of the substances present in the larvae gut content did not affect the enzyme activity. Interestingly, an increase of activity was observed when PlyPl23 was previously incubated in royal jelly. Furthermore, in vivo safety evaluation assays demonstrated that this enzyme is not toxic to the bee lar- vae. The present work describes for the first time an endolysin encoded in a P. larvae phage that presents high potential to integrate a commercial product to control the problematic American foulbrood. |
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The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbroodBacteriophageEndolysinPaenibacillus larvaeCiências Médicas::Biotecnologia MédicaScience & TechnologyEndolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of the reproduction cycle of bacteriophages, have great potential to control Gram-pos- itive pathogens. This work describes the characterization of a novel endolysin (PlyPl23) encoded on the genome of Paenibacillus larvae phage phiIBB_Pl23 with high potential to control American foulbrood. This bacterial disease, caused by P. larvae, is widespread in North America and Europe and causes important economic losses in apiculture. The restric- tion to antibiotic residues in honey imposed by the EU legislation hinders its therapeutic use to combat American foulbrood and enforces the development of alternative antimicrobial methods. The new endolysin described herein has an N-acetylmuramoyl-L-alanine ami- dase catalytic domain and exhibits a broad-spectrum activity against common P. larvae genotypes. Moreover, the enzyme displays high antimicrobial activity in a range of pH that matches environmental conditions (pH between 5.0 and 7.0), showing its feasible applica- tion in the field. At pH 7.0, a concentration of 0.2 M of enzyme was enough to lyse 104 CFU.mL-1 of P. larvae in no more than 2 h. The presence of sucrose and of the substances present in the larvae gut content did not affect the enzyme activity. Interestingly, an increase of activity was observed when PlyPl23 was previously incubated in royal jelly. Furthermore, in vivo safety evaluation assays demonstrated that this enzyme is not toxic to the bee lar- vae. The present work describes for the first time an endolysin encoded in a P. larvae phage that presents high potential to integrate a commercial product to control the problematic American foulbrood.FCT—Foundation for Science and Technology strategic funding of UID/BIO/04469/2013 unit and the European Community fund FEDER, through Program COMPETE, under the scope of the Projects FCOMP-01-0124-FEDER-007025 (PTDC/AMB/68393/2006), PEst-OE/EQB/LA0023/2013, PEST-C/FIS/UI607/2013, RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462).Public Library of ScienceUniversidade do MinhoOliveira, A.Leite, MartaKluskens, LeonSantos, Sílvio Roberto BrancoMelo, Luís D. R.Azeredo, Joana2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37259engOliveira, A.; Leite, M.; Kluskens, Leon; Santos, S.; Melo, L.; Azeredo, Joana, The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood. PLoS One, 10(7), e0136331, 20151932-62031932-620310.1371/journal.pone.013209526167894http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132095info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:08Zoai:repositorium.sdum.uminho.pt:1822/37259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:03.250798Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
title |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
spellingShingle |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood Oliveira, A. Bacteriophage Endolysin Paenibacillus larvae Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
title_full |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
title_fullStr |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
title_full_unstemmed |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
title_sort |
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood |
author |
Oliveira, A. |
author_facet |
Oliveira, A. Leite, Marta Kluskens, Leon Santos, Sílvio Roberto Branco Melo, Luís D. R. Azeredo, Joana |
author_role |
author |
author2 |
Leite, Marta Kluskens, Leon Santos, Sílvio Roberto Branco Melo, Luís D. R. Azeredo, Joana |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, A. Leite, Marta Kluskens, Leon Santos, Sílvio Roberto Branco Melo, Luís D. R. Azeredo, Joana |
dc.subject.por.fl_str_mv |
Bacteriophage Endolysin Paenibacillus larvae Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
Bacteriophage Endolysin Paenibacillus larvae Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Endolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of the reproduction cycle of bacteriophages, have great potential to control Gram-pos- itive pathogens. This work describes the characterization of a novel endolysin (PlyPl23) encoded on the genome of Paenibacillus larvae phage phiIBB_Pl23 with high potential to control American foulbrood. This bacterial disease, caused by P. larvae, is widespread in North America and Europe and causes important economic losses in apiculture. The restric- tion to antibiotic residues in honey imposed by the EU legislation hinders its therapeutic use to combat American foulbrood and enforces the development of alternative antimicrobial methods. The new endolysin described herein has an N-acetylmuramoyl-L-alanine ami- dase catalytic domain and exhibits a broad-spectrum activity against common P. larvae genotypes. Moreover, the enzyme displays high antimicrobial activity in a range of pH that matches environmental conditions (pH between 5.0 and 7.0), showing its feasible applica- tion in the field. At pH 7.0, a concentration of 0.2 M of enzyme was enough to lyse 104 CFU.mL-1 of P. larvae in no more than 2 h. The presence of sucrose and of the substances present in the larvae gut content did not affect the enzyme activity. Interestingly, an increase of activity was observed when PlyPl23 was previously incubated in royal jelly. Furthermore, in vivo safety evaluation assays demonstrated that this enzyme is not toxic to the bee lar- vae. The present work describes for the first time an endolysin encoded in a P. larvae phage that presents high potential to integrate a commercial product to control the problematic American foulbrood. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 2015-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/37259 |
url |
http://hdl.handle.net/1822/37259 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, A.; Leite, M.; Kluskens, Leon; Santos, S.; Melo, L.; Azeredo, Joana, The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control American foulbrood. PLoS One, 10(7), e0136331, 2015 1932-6203 1932-6203 10.1371/journal.pone.0132095 26167894 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132095 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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