A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/42183 |
Resumo: | "Available online 22 June 2016" |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complexFIESCSecondary metabolitesPhylogenyTRI genesScience & Technology"Available online 22 June 2016"DNA-based phylogenetic analyses have resolved the fungal genus Fusarium into multiple species complexes. The F. incarnatum-equiseti species complex (FIESC) includes fusaria associated with several diseases of agriculturally important crops, including cereals. Although members of FIESC are considered to be only moderately aggressive, they are able to produce a diversity of mycotoxins, including trichothecenes, which can accumulate to harmful levels in cereals. High levels of cryptic speciation have been detected within the FIESC. As a result, it is often necessary to use approaches other than morphological characterization to distinguish species. In the current study, we used a polyphasic approach to characterize a collection of 69 FIESC isolates recovered from cereals in Europe, Turkey, and North America. In a species phylogeny inferred from nucleotide sequences from four housekeeping genes, 65 of the isolates were resolved within the Equiseti clade of the FIESC, and four isolates were resolved within the Incarnatum clade. Seven isolates were resolved as a genealogically exclusive lineage, designated here as FIESC 31. Phylogenies based on nucleotide sequences of trichothecene biosynthetic genes and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) were largely concordant with phylogeny inferred from the housekeeping gene. Finally, Liquid Chromatography (Time-Of-Flight) Mass Spectrometry [LC-(TOF-)MS(/MS)] revealed variability in mycotoxin production profiles among the different phylogenetic species investigated in this study.This work was supported by the EU project EC KBBE-2007-222690-2 MYCORED.ElsevierUniversidade do MinhoVillani, AlessandraMoretti, AntonioDe Saeger, SarahHan, ZhengDi Mavungu, Jose DianaSoares, Célia Maria GonçalvesProctor, Robert H.Venâncio, ArmandoLima, NelsonStea, GaetanoPaciolla, CostantinoLogrieco, Antonio F.Susca, Antonia20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42183engVillani, Alessandra; Moretti, Antonio; De Saeger, Sarah; Han, Zheng; Di Mavungu, Jose Diana; Soares, C.; Proctor, Robert H.; Venâncio, Armando; Lima, Nelson; Stea, Gaetano; Paciolla, Costantino; Logrieco, Antonio F.; Susca, Antonia, A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex. International Journal of Food Microbiology, 234, 24-35, 20160168-16050168-160510.1016/j.ijfoodmicro.2016.06.02327376677http://www.journals.elsevier.com/international-journal-of-food-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:19Zoai:repositorium.sdum.uminho.pt:1822/42183Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:33.471542Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| title |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| spellingShingle |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex Villani, Alessandra FIESC Secondary metabolites Phylogeny TRI genes Science & Technology |
| title_short |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| title_full |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| title_fullStr |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| title_full_unstemmed |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| title_sort |
A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
| author |
Villani, Alessandra |
| author_facet |
Villani, Alessandra Moretti, Antonio De Saeger, Sarah Han, Zheng Di Mavungu, Jose Diana Soares, Célia Maria Gonçalves Proctor, Robert H. Venâncio, Armando Lima, Nelson Stea, Gaetano Paciolla, Costantino Logrieco, Antonio F. Susca, Antonia |
| author_role |
author |
| author2 |
Moretti, Antonio De Saeger, Sarah Han, Zheng Di Mavungu, Jose Diana Soares, Célia Maria Gonçalves Proctor, Robert H. Venâncio, Armando Lima, Nelson Stea, Gaetano Paciolla, Costantino Logrieco, Antonio F. Susca, Antonia |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Villani, Alessandra Moretti, Antonio De Saeger, Sarah Han, Zheng Di Mavungu, Jose Diana Soares, Célia Maria Gonçalves Proctor, Robert H. Venâncio, Armando Lima, Nelson Stea, Gaetano Paciolla, Costantino Logrieco, Antonio F. Susca, Antonia |
| dc.subject.por.fl_str_mv |
FIESC Secondary metabolites Phylogeny TRI genes Science & Technology |
| topic |
FIESC Secondary metabolites Phylogeny TRI genes Science & Technology |
| description |
"Available online 22 June 2016" |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42183 |
| url |
http://hdl.handle.net/1822/42183 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Villani, Alessandra; Moretti, Antonio; De Saeger, Sarah; Han, Zheng; Di Mavungu, Jose Diana; Soares, C.; Proctor, Robert H.; Venâncio, Armando; Lima, Nelson; Stea, Gaetano; Paciolla, Costantino; Logrieco, Antonio F.; Susca, Antonia, A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex. International Journal of Food Microbiology, 234, 24-35, 2016 0168-1605 0168-1605 10.1016/j.ijfoodmicro.2016.06.023 27376677 http://www.journals.elsevier.com/international-journal-of-food-microbiology/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
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RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133034783440896 |