First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/17672 https://doi.org/10.1111/efp.12162 |
Resumo: | The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra. |
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First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europepinewood nematode;Bursaphelenchus xylophilusThe pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra.Forest Pathology2016-03-01T14:30:40Z2016-03-012014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/17672http://hdl.handle.net/10174/17672https://doi.org/10.1111/efp.12162porlurdes.inacio@iniav.ptfilomena.nobrega@iniav.ptpvieira@uevora.ptluis.bonifacio@iniav.ptedmundo.sousa@iniav.ptmmota@uevora.pt581Inácio, LurdesNobrega, FilomenaVieira, PauloBonifacio, LuisSousa, EdmundoMota, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:05:16Zoai:dspace.uevora.pt:10174/17672Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:09:43.955684Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
title |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
spellingShingle |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe Inácio, Lurdes pinewood nematode; Bursaphelenchus xylophilus |
title_short |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
title_full |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
title_fullStr |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
title_full_unstemmed |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
title_sort |
First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe |
author |
Inácio, Lurdes |
author_facet |
Inácio, Lurdes Nobrega, Filomena Vieira, Paulo Bonifacio, Luis Sousa, Edmundo Mota, Manuel |
author_role |
author |
author2 |
Nobrega, Filomena Vieira, Paulo Bonifacio, Luis Sousa, Edmundo Mota, Manuel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Inácio, Lurdes Nobrega, Filomena Vieira, Paulo Bonifacio, Luis Sousa, Edmundo Mota, Manuel |
dc.subject.por.fl_str_mv |
pinewood nematode; Bursaphelenchus xylophilus |
topic |
pinewood nematode; Bursaphelenchus xylophilus |
description |
The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01T00:00:00Z 2016-03-01T14:30:40Z 2016-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/17672 http://hdl.handle.net/10174/17672 https://doi.org/10.1111/efp.12162 |
url |
http://hdl.handle.net/10174/17672 https://doi.org/10.1111/efp.12162 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
lurdes.inacio@iniav.pt filomena.nobrega@iniav.pt pvieira@uevora.pt luis.bonifacio@iniav.pt edmundo.sousa@iniav.pt mmota@uevora.pt 581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Forest Pathology |
publisher.none.fl_str_mv |
Forest Pathology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136579480977408 |