First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe

Detalhes bibliográficos
Autor(a) principal: Inácio, Lurdes
Data de Publicação: 2014
Outros Autores: Nobrega, Filomena, Vieira, Paulo, Bonifacio, Luis, Sousa, Edmundo, Mota, Manuel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/17672
https://doi.org/10.1111/efp.12162
Resumo: The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra.
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spelling First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europepinewood nematode;Bursaphelenchus xylophilusThe pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra.Forest Pathology2016-03-01T14:30:40Z2016-03-012014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/17672http://hdl.handle.net/10174/17672https://doi.org/10.1111/efp.12162porlurdes.inacio@iniav.ptfilomena.nobrega@iniav.ptpvieira@uevora.ptluis.bonifacio@iniav.ptedmundo.sousa@iniav.ptmmota@uevora.pt581Inácio, LurdesNobrega, FilomenaVieira, PauloBonifacio, LuisSousa, EdmundoMota, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:05:16Zoai:dspace.uevora.pt:10174/17672Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:09:43.955684Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
title First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
spellingShingle First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
Inácio, Lurdes
pinewood nematode;
Bursaphelenchus xylophilus
title_short First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
title_full First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
title_fullStr First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
title_full_unstemmed First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
title_sort First detection of Bursaphelenchus xylophilus associated with Pinus nigra in Portugal and in Europe
author Inácio, Lurdes
author_facet Inácio, Lurdes
Nobrega, Filomena
Vieira, Paulo
Bonifacio, Luis
Sousa, Edmundo
Mota, Manuel
author_role author
author2 Nobrega, Filomena
Vieira, Paulo
Bonifacio, Luis
Sousa, Edmundo
Mota, Manuel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Inácio, Lurdes
Nobrega, Filomena
Vieira, Paulo
Bonifacio, Luis
Sousa, Edmundo
Mota, Manuel
dc.subject.por.fl_str_mv pinewood nematode;
Bursaphelenchus xylophilus
topic pinewood nematode;
Bursaphelenchus xylophilus
description The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. This species has been reported from the United States of America and Asia associated with important forestry tree species, including pine, spruce, fir, larch and other conifers. However, in Europe, the devastating epidemics centred in Portugal’s mainland, and the most recent detections in Madeira Island and in Spain were restricted to Pinus pinaster alone. During the 2013 national survey, wilted Pinus nigra trees, from the central region of Portugal, were sampled. A Bursaphelenchus species was found in very high numbers, with an average up to 5000 nematodes per 10 g of wood, collected from different pine trees. Morphological identification of those specimens, including the presence of a bursa in the male tail, four lateral incisures, shape of spicules, presence of a vulva flap and female rounded tail, was in agreement with the PWN description. Species identification was confirmed also using species-specific primers targeting the ITS region of the ribosomal DNA to the PWN. Herein, B. xylophilus is reported for the first time associated with declining P. nigra in Portugal, and in Europe. Simultaneously, the insect vector Monochamus galloprovincialis was also detected colonizing the PWN-infected P. nigra.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01T00:00:00Z
2016-03-01T14:30:40Z
2016-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/17672
http://hdl.handle.net/10174/17672
https://doi.org/10.1111/efp.12162
url http://hdl.handle.net/10174/17672
https://doi.org/10.1111/efp.12162
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv lurdes.inacio@iniav.pt
filomena.nobrega@iniav.pt
pvieira@uevora.pt
luis.bonifacio@iniav.pt
edmundo.sousa@iniav.pt
mmota@uevora.pt
581
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Forest Pathology
publisher.none.fl_str_mv Forest Pathology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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