Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces

Detalhes bibliográficos
Autor(a) principal: Pereira, Ana Margarida Macedo Bernardes
Data de Publicação: 2021
Outros Autores: Gomes, Diana Cristina Serra, da Costa, André, Dias, Simoni Campos, Casal, Margarida, Machado, Raul
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/74226
Resumo: Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in <i>Escherichia coli</i>, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.
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spelling Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfacesAntimicrobial resistanceAntimicrobial surfacesAntimicrobial filmsRecombinant protein polymersSilk-elastin proteinsElastin-like recombinamersAntimicrobial peptidesScience & TechnologyAntibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in <i>Escherichia coli</i>, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.This work was supported by the “Contrato-Programa” UIDB/04050/2020, project FunBioPlas (ERA-IB-2-6/0004/2014) and project FUN2CYT (POCI-01-0145-FEDER-030568) funded by Portugal national funds through the Fundação para a Ciência e a Tecnologia (FCT I.P.). A.M.P. acknowledges the Doctoral Programme in Applied and Environmental Microbiology (DP_AEM) and FCT I.P. for the PD/BD/113811/2015 grant. R.M. acknowledges FCT I.P. for funding in the scope of the Scientific Employment Stimulus instrument (CEECIND/00526/2018).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoPereira, Ana Margarida Macedo BernardesGomes, Diana Cristina Serrada Costa, AndréDias, Simoni CamposCasal, MargaridaMachado, Raul2021-06-092021-06-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/74226engPereira, A.M.; Gomes, D.; da Costa, A.; Dias, S.C.; Casal, M.; Machado, R. Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces. Appl. Sci. 2021, 11, 5352. https://doi.org/10.3390/app111253522076-341710.3390/app11125352https://www.mdpi.com/2076-3417/11/12/5352info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:42:36Zoai:repositorium.sdum.uminho.pt:1822/74226Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:42:36Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
title Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
spellingShingle Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
Pereira, Ana Margarida Macedo Bernardes
Antimicrobial resistance
Antimicrobial surfaces
Antimicrobial films
Recombinant protein polymers
Silk-elastin proteins
Elastin-like recombinamers
Antimicrobial peptides
Science & Technology
title_short Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
title_full Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
title_fullStr Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
title_full_unstemmed Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
title_sort Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces
author Pereira, Ana Margarida Macedo Bernardes
author_facet Pereira, Ana Margarida Macedo Bernardes
Gomes, Diana Cristina Serra
da Costa, André
Dias, Simoni Campos
Casal, Margarida
Machado, Raul
author_role author
author2 Gomes, Diana Cristina Serra
da Costa, André
Dias, Simoni Campos
Casal, Margarida
Machado, Raul
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, Ana Margarida Macedo Bernardes
Gomes, Diana Cristina Serra
da Costa, André
Dias, Simoni Campos
Casal, Margarida
Machado, Raul
dc.subject.por.fl_str_mv Antimicrobial resistance
Antimicrobial surfaces
Antimicrobial films
Recombinant protein polymers
Silk-elastin proteins
Elastin-like recombinamers
Antimicrobial peptides
Science & Technology
topic Antimicrobial resistance
Antimicrobial surfaces
Antimicrobial films
Recombinant protein polymers
Silk-elastin proteins
Elastin-like recombinamers
Antimicrobial peptides
Science & Technology
description Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in <i>Escherichia coli</i>, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-09
2021-06-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/74226
url http://hdl.handle.net/1822/74226
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pereira, A.M.; Gomes, D.; da Costa, A.; Dias, S.C.; Casal, M.; Machado, R. Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces. Appl. Sci. 2021, 11, 5352. https://doi.org/10.3390/app11125352
2076-3417
10.3390/app11125352
https://www.mdpi.com/2076-3417/11/12/5352
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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